Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,219,961 A→T 8.3% F166L (TTT→TTA)  CJ019_02103 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,219,9610AT 8.3% 143.3 / 4.4 48F166L (TTT→TTA) CJ019_02103hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/17);  new base T (2/2);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

CCAGAATGCCGCTTCGTTTTCATATCTGATATCCTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGCTTGCA  >  KT2440/2219835‑2220091
                                                                                                                              |                                                                                                                                  
ccAGAATGCCGCTTCGTTTTCATATCTGATATCCTTTATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTcg                                                                                                                            >  3:286698/1‑135 (MQ=255)
ccAGAATGCCGCTTCGTTTTCATATCTGATATCCTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTcg                                                                                                                            >  4:253228/1‑135 (MQ=255)
ccAGAATGCCGCTTCGTTTTCATATCTGATATCCTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTcg                                                                                                                            >  6:170249/1‑135 (MQ=255)
ccAGAATGCCGCTTCGTTTTCAAATCTGATATGCTTAATAATGAAGATGCTTTTACCAGCGGGATTTGGTAGTGGGGGAGTAAGGGTTGATATTTACCGCTTGCTTCTAGTGCGGACGTAAGAGGGAAAGTGTcg                                                                                                                            >  6:12520/1‑135 (MQ=255)
ccAGAATGCCGCTTCGGTTTGATATCTGATATCCTTAATAATGAAGATGCTTTTACCGGCGGCATTTGTTAGGGGGCGAGTAGGGGGTGATATTGCACGCGTGCTTCTAGTGCTGACGGAAGAGGGCTAGTCTcg                                                                                                                            >  7:317436/1‑135 (MQ=255)
         cGCTTCGTTTTCATATCTGATATCCTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGGGGGCGAGTAGGGGTGGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATa                                                                                                                   <  8:106681/135‑1 (MQ=255)
            ttCGTTTTCATATCTGATATCCTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCg                                                                                                                >  5:208956/1‑135 (MQ=255)
                         cTGATATCCTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGc                                                                                                    <  1:207649/134‑1 (MQ=255)
                             tatCCTTAATAATGAAGATGCTTTTACCAGCGGCGTTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGaa                                                                                                 <  4:265596/133‑1 (MQ=255)
                             tatCCTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGaa                                                                                                 <  5:230568/133‑1 (MQ=255)
                             catCCTTAAACATGAAGAGGCTTTTACACGCGGCTTTTGTTAGTGGCCGAGTAGGGGTTGATATTGCACGCTGGCTTCTCGTGCTGACGTACGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGaa                                                                                                 <  5:244264/132‑1 (MQ=255)
                             aatCCTTAATAATGAAGATGCTTTTCCCAGCGGCATTTGTTAGGGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTACGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGaa                                                                                                 <  1:275218/132‑1 (MQ=255)
                                ccTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGaa                                                                                                 <  7:227254/130‑1 (MQ=255)
                                ccTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGaa                                                                                                 >  8:227254/1‑130 (MQ=255)
                                  ttAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTcc                                                                                          >  5:145783/1‑135 (MQ=255)
                                    aataatGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCtt                                                                                        >  3:9994/1‑135 (MQ=255)
                                        atGAAGATGCTTTTACCCGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTggg                                                                                    <  7:190614/135‑1 (MQ=255)
                                         tGAAGTTGCTTTTACCCGGGGCATTTTTTCGTGGGCGTGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTggg                                                                                    <  6:208956/134‑1 (MQ=255)
                                                 cTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGTAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGtt                                                                            >  5:297270/1‑134 (MQ=255)
                                                 cTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGTAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGtt                                                                            >  5:292176/1‑134 (MQ=255)
                                                    ttACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCa                                                                         >  2:8271/1‑134 (MQ=255)
                                                     tACGAGCGGCATTTGTTTGTGGGCGAGTAGGGGTTGATTTTGCCCGCTTGCTTCTAGTGCTGACGTATGAGGGTAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAg                                                                        <  6:292176/134‑1 (MQ=255)
                                                     tACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGTAAGGCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAg                                                                        <  6:297270/134‑1 (MQ=255)
                                                     tACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAg                                                                        <  6:124342/134‑1 (MQ=255)
                                                        cAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATg                                                                    >  6:31768/1‑135 (MQ=255)
                                                              cATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCct                                                              >  3:320427/1‑135 (MQ=255)
                                                               aTTTGTTAGTGGCCGAGTCGGGGTTGATTTTGCACGCTTGCTTCTTGTGCTGAGGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCATCAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCctc                                                             <  8:273473/135‑1 (MQ=255)
                                                                    ttAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCAAGTGCTGAGTTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGcc                                                         <  3:296770/134‑1 (MQ=255)
                                                                                            ttGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCATTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGttt                                >  2:154994/1‑135 (MQ=255)
                                                                                                aCGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATgg                                                                   <  1:240045/96‑1 (MQ=255)
                                                                                                aCGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATgg                                                                   >  2:240045/1‑96 (MQ=255)
                                                                                                aCGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGc                            >  8:250455/1‑135 (MQ=255)
                                                                                                aCGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGc                            >  4:315582/1‑135 (MQ=255)
                                                                                                  gcttgcttCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAAtt                                                                                                      <  3:100578/59‑1 (MQ=255)
                                                                                                  gcttgcttCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAAtt                                                                                                      >  4:100578/1‑59 (MQ=255)
                                                                                                  gcttgcttCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCt                           >  3:122409/1‑134 (MQ=255)
                                                                                                       cttCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAg                                                                        >  7:71896/1‑84 (MQ=255)
                                                                                                       cttCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAg                                                                        <  8:71896/84‑1 (MQ=255)
                                                                                                       cttCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAg                       <  6:92544/133‑1 (MQ=255)
                                                                                                        ttCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTa                    >  6:336549/1‑135 (MQ=255)
                                                                                                            aGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATcc                >  5:74119/1‑135 (MQ=255)
                                                                                                                 tGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCa             >  5:176979/1‑133 (MQ=255)
                                                                                                                     gTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGc       >  2:211836/1‑135 (MQ=255)
                                                                                                                     gTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGc       >  5:81209/1‑135 (MQ=255)
                                                                                                                     gTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGc       >  1:179106/1‑135 (MQ=255)
                                                                                                                     gTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGc       >  4:47061/1‑135 (MQ=255)
                                                                                                                        agagGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGCtt     >  8:288836/1‑134 (MQ=255)
                                                                                                                         gcggcaaaGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGCTTGc   <  6:33883/130‑1 (MQ=255)
                                                                                                                          agGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGCTTGCa  <  7:250455/135‑1 (MQ=255)
                                                                                                                              |                                                                                                                                  
CCAGAATGCCGCTTCGTTTTCATATCTGATATCCTTAATAATGAAGATGCTTTTACCAGCGGCATTTGTTAGTGGGCGAGTAGGGGTTGATATTGCACGCTTGCTTCTAGTGCTGACGTAAGAGGGAAAGTCTCGCGACAGATAGCGATCAACAATTGCGAACATCTCCTTTGGGGTGATGTTCCAGAATGGGTCCTCTGCCGCTCGGAGATCATCTTGCCAAGTTTGGGCTTCAGTTAATCCCCAGAAGGCTTGCA  >  KT2440/2219835‑2220091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑