Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,264,985	T→C	11.6%	intergenic (+136/‑6)	PP_RS08755 → / → PP_RS08755	tRNA‑Asp/tRNA‑Asp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,264,985	0	T	C	11.6%	113.6 / 3.1	43	intergenic (+136/‑6)	PP_RS08755/PP_RS08755	tRNA‑Asp/tRNA‑Asp
Reads supporting (aligned to +/- strand):  ref base T (18/19);  new base C (2/3);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTCAAGGTCCCTAGAACACCTTGAAGCAAACACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTGACGCAACGTTAAA  >  KT2440/2264855‑2265110                                                                                                                                   |                                                                                                                              gTTTCAAGGTCCCTAGAACACCTTGAAGCAAACTCTATAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGAt                                                                                                                            <  7:166563/134‑1 (MQ=255) gTTTCAAGGTCCCTAGAACACCTTGAAGCAAACACAAGAGACGCGAGCGCGTTATCGCTTTTTTTGTGCCAGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGAt                                                                                                                            <  3:98600/134‑1 (MQ=255)  tttCAAGGTCCCTAGAACACCTTGAAGCAAACACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCCGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATa                                                                                                                           <  5:244524/134‑1 (MQ=255)  tatCAAGGTCCCTAGAACACCTTGAAGCAAACACAAGAGAAGCGATGGCGTTATCGCTTTTTTTGTGGCTGACCTGAGGCCATCTCGCCGGAAGGGTTGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATa                                                                                                                           <  7:296384/132‑1 (MQ=255)  attCAAGGTCCCTACAACACCTTGAAGCCAACCCAAGTGAAGCGATCTCGTTATCTCTTTTTTGGTGCCGGGCCTGAGGCCATCGCGGCGGAAGGGTAGACCCATGTTTCAATCGGGCGCAAGCCTGAATGATa                                                                                                                           <  3:18597/133‑1 (MQ=255)           cccAAGAACACCTTGAAGCAAACACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTa                                                                                                                 <  4:240657/135‑1 (MQ=255)             cTAGAACCCCTTGAAGCAAACACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCGGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGt                                                                                                               <  1:69052/135‑1 (MQ=255)                 aaCTCCTTGAAGCAAACTGAAGTGAAGCGATCGCGTTATGGCTTTTTTTGTGGCTGCCCTGATGCGTTCGCGCCGGATGGGTAGACCCAGGTTTCAATCGGGCTCAAGCCTGAATGATACGCAGCGGTAGTTCa                                                                                                            <  7:212343/134‑1 (MQ=37)                   ctccTGGAAGCAACCACACTCTATGCGCTCGCGTTATGGCTTTTGTTGGGCCGGCCCTGCGGCCTGCGCGTCGGATGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAAGGATACGCCGCGGTAGTTCAGTc                                                                                                         <  1:113340/133‑1 (MQ=16)                       ttGAAGCAAACACAAGAGAAGCGATATAGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCgg                                                                                                       >  8:218278/1‑133 (MQ=255)                         gAAGCAAACACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGttg                                                                                                    >  2:208651/1‑133 (MQ=255)                         gAAGCAAACACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTa                                                                                                    >  4:249673/1‑134 (MQ=255)                           aGCAAACACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGa                                                                                                  >  5:280099/1‑134 (MQ=255)                               aaCACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATAcc                                                                                             <  6:283053/135‑1 (MQ=255)                                  acaAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCgga                                                                                          >  4:190446/1‑134 (MQ=255)                                   caAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGcc                                                                                         >  1:24941/1‑135 (MQ=255)                                       agaAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTc                                                                                     >  1:28538/1‑135 (MQ=255)                                          aGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACg                                                                                  >  1:204831/1‑135 (MQ=255)                                                  cgTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGGCGGTTAGAATACCGGCCTGTCCCGCCgggggg                                                                          >  1:117530/1‑134 (MQ=255)                                                   gTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGACACGCCGGGGGTc                                                                         <  2:24941/135‑1 (MQ=255)                                                   gTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACAGGCCTGTCACGCCGGGGGTc                                                                         >  1:270830/1‑135 (MQ=255)                                                               tttGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACg                                                                                                                         >  4:103724/1‑75 (MQ=255)                                                               tttGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACg                                                                                                                         <  3:103724/75‑1 (MQ=255)                                                                ttGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTc                                                            >  1:277558/1‑135 (MQ=255)                                                                                gCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTGCGCTgcggcct                                            >  3:2752/1‑131 (MQ=255)                                                                                 ccATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCggg                                                                    <  6:265457/110‑1 (MQ=255)                                                                                 ccATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCggg                                                                    >  5:265457/1‑110 (MQ=255)                                                                                  ctgctcgcCGGTTGGGCAGACCCCGGTTTCAATCGGGCGCAAGCCTGAATGATAGGCAGGGGTCGTTCAGTGGGTTACAATACCGGCCTGGCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCt                                          <  1:25022/130‑1 (MQ=11)                                                                                     cgcgCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCTGGTTCGAGTCCCGACCGCTTCGCCagcata                                        >  5:75545/1‑129 (MQ=17)                                                                                                   tgACCCAGGTTTCATTCGGGCGCAAGCCGGAACGATACGCAGCGGTAGTTCTGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCAt                         <  8:259740/134‑1 (MQ=255)                                                                                                    gagccATGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTATTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCAt                         <  4:251714/131‑1 (MQ=255)                                                                                                        ccagTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCTAGTCCCGTACCCGGCGCCAGCTTCGAGTCACGGGCCCATGAACg                    >  2:102632/4‑135 (MQ=11)                                                                                                        ccagTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGGTTCAAGGGCCCATGAAcc                    >  8:253677/4‑134 (MQ=0)                                                                                                            tttCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCtt                >  3:95759/1‑135 (MQ=255)                                                                                                             ttCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATCCCGGCCGGGCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTg               <  3:249673/135‑1 (MQ=255)                                                                                                             ttCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTg               <  7:218278/135‑1 (MQ=255)                                                                                                             tgcattCGCGCGCAAGCCTGAATGATACGCAGCGTTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTg               <  6:75545/130‑1 (MQ=255)                                                                                                             tccAATCGGGCGCAAGCCTGAACTATAGGCCGCGGTAGTTCAGGCGGTTATAATACCGGCCTGCCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTg               <  6:109084/133‑1 (MQ=14)                                                                                                             ctctctCGGGCGCACGCGTGATCGAGCCGCTGCGGTAGTGCCGTCGGTTAGAATCCCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTg               <  1:252449/130‑1 (MQ=14)                                                                                                              tCAATCGGGCGCAAGCCTGAATGATACGCTGCGGTAGTGCATTCTGTAAGAATACCGGGCTGTCACGCCGGGGGTCTCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTg               <  5:152087/134‑1 (MQ=14)                                                                                                              gaaaccGGCCGCCAGCCTGAATTATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTGa              <  3:218709/130‑1 (MQ=14)                                                                                                                        gcAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCGGCGGCATCTTCGCGTCAAGGCCCCAAGGACGCCGTGACGCAACGtt     >  1:209469/1‑134 (MQ=14)                                                                                                                          aaGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGGCCGCTGCGCCCTCTTCGCTTCAACGCCCCATGAACCCCTTGTCCCAACCTTtaa  >  3:19415/1‑135 (MQ=14)                                                                                                                                   |                                                                                                                              GTTTCAAGGTCCCTAGAACACCTTGAAGCAAACACAAGAGAAGCGATCGCGTTATCGCTTTTTTTGTGCCTGCCCTGAGGCCATCGCGCCGGAAGGGTAGACCCAGGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGCTTCAAGGCCCCATGAACGCCTTGACGCAACGTTAAA  >  KT2440/2264855‑2265110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑