| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,744,444 | A→C | 33.6% | N64H (AAC→CAC) | czcC_2 → | Cobalt‑zinc‑cadmium resistance protein CzcC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,744,444 | 0 | A | C | 33.6% | 41.7 / 2.0 | 30 | N64H (AAC→CAC) | czcC_2 | Cobalt‑zinc‑cadmium resistance protein CzcC |
| Reads supporting (aligned to +/- strand): ref base A (8/11); new base C (9/2); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCCCAGGCCAGCCAGGTGCTGAGCCTGCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCGCGGCGCACGTCTCGCCGTGGCGGGTGCCGGCCAG > KT2440/2744310‑2744572 | aCGCCCAGGCCAGCCAGGTGCTGAGCCTGCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCa < 5:128956/135‑1 (MQ=255) ccAGGCCAGCCAGGTGCTGAGCCTGCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAAccc < 4:167309/135‑1 (MQ=255) agccagGTGCTGAGCCTGCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGACCGGCGTCCGGCCGGGCTGCTCCCCCACCCGGAGCTg > 1:248976/1‑135 (MQ=255) tGAGCCTGCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGGAATTGGCATTGCCGCTGGCGCACGGCGTCCGGCCGGGCTGGTCCCCCACCCGGCGCTGGGCTGGGAgg > 3:306989/1‑135 (MQ=255) tGAGCCTGCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCCACCCGGCGCTGGCCTGGGAgg > 4:31035/1‑135 (MQ=255) cTGCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGGAATTGGGATTGGCGATGGCGCACGGCGGCAGGCCGGGCTGCTCCCCCCCCCGGCGCTGGGCGGGGGGGTCgcg > 7:126411/1‑133 (MQ=255) gCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCCACCCGGAGCTGGCCTGGGAGGTCGAGGa > 2:8066/1‑135 (MQ=255) ccAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGCGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGTACCGCGTCAGGCCGGGCTGCTCCCCCACCCGGAGCTGGCCTGGGAGGTCGAgggccc > 4:30385/1‑131 (MQ=255) ccAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGCACGGCGTCAGGCCGGGCTGCTCCCCCACCCGGAGCTGGCCTGGGAGGTCGCGGaccc > 7:15103/1‑133 (MQ=255) ccAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGCACGGCGTCAGGCCGGGCTGCGCCCCCACCCGGCGCTGGCCTGGGAGGGCGCGGacaa > 4:247136/1‑134 (MQ=255) ccAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGacac > 6:77864/1‑135 (MQ=255) gcgcTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACccgc < 8:87054/135‑1 (MQ=255) cTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGGCGATGGCGGACGGCGGCAGGCCGGGCTGCTCCCCAACCCGGCGCTGGGCTGGGGGGGCGCGGACAcccccccc > 1:150120/1‑133 (MQ=255) cTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGCACGGCGTCAGGCCGGGCTGCGCCCCAACCCCGCGCTGGCCGGGGCGGGCGCGGGCCCCccccgc > 1:154672/1‑135 (MQ=255) cTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCcgccgc < 4:28133/135‑1 (MQ=255) aCCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGATATTGGCATTGCCGATGGCGCACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCcgcgcc > 5:169142/1‑133 (MQ=255) ccgccgCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAAATGGCATTGCCGATGGCGCACGGCCTCAGGCCGGGCTGCTCCCCACCCCGGCGCTGGCCTGGGATGGCGACGACCCCCGCCGCGacg > 3:256866/1‑134 (MQ=255) gccgcTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGCACGGCGTCAGGCCGGGCTGCTCCCCCACCCGGAGCTGGCCTGGGCGGGCGAGGCCACCCGCCGCGCCAcc > 8:87479/1‑135 (MQ=255) cgcTTTCGCCCAGAACCCGTAGCTGGCTGCAGCGGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCGGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAg < 2:126852/135‑1 (MQ=255) tttCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGcac < 6:335818/135‑1 (MQ=255) gctggctgCAGCTGGCCGTTAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTcag < 4:4323/135‑1 (MQ=255) cTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTgg < 3:143186/135‑1 (MQ=255) tGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGa > 4:138384/1‑135 (MQ=255) ttcccGAGGGCGAACGGCGGCCGGCCGGGCTGCGCCCCACCCCGGAGCTGGCCTGGGAGGGCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGcgcg < 5:273220/132‑1 (MQ=255) tGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCgcgg < 2:248976/135‑1 (MQ=255) aGGCCGGGCTGCTCCCCCACCCGGAGCGGGCCGGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCGCGGCGCACGTCTCGCCGTGGCgg < 4:123521/135‑1 (MQ=255) aGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCGCGGCGCACGTCTCGCCGTGGCgg < 4:118263/135‑1 (MQ=255) tgctCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCGCGGCGCACGtc < 6:326932/114‑1 (MQ=255) tgctCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCGCGGCGCACGtc > 5:326932/1‑114 (MQ=255) tgctCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCGCGGCGCACGTCTCGCCGTGGCGGGTGCCgg > 2:55162/1‑133 (MQ=255) ctCCCCCACCCGGAGCGGGCCGGGGAGGGCGAGGACACCCGCCGCGACACCAGCACCACCACCGGCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCGCGGCGCACGTCTCGCCGTGGCGGGTGCCggccag < 2:291091/135‑1 (MQ=255) | ACGCCCAGGCCAGCCAGGTGCTGAGCCTGCCCCAGGCGCTTACCGCCGCTTTCGCCCAGAACCCGGAGCTGGCTGCAGCTGGCCGTGAAATTGGCATTGCCGATGGCGAACGGCGTCAGGCCGGGCTGCTCCCCAACCCGGAGCTGGCCTGGGAGGTCGAGGACACCCGCCGCGACACCAGCACCACCACCGTCACCCTCAGCCAGCCGCTGGAGCTGGGCGGGAAGCGCGGCGCACGTCTCGCCGTGGCGGGTGCCGGCCAG > KT2440/2744310‑2744572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |