| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,839,289 | C→T | 100% | intergenic (‑117/‑88) | CJ019_02692 ← / → CJ019_02693 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,839,289 | 0 | C | T | 100.0% | 143.7 / NA | 39 | intergenic (‑117/‑88) | CJ019_02692/CJ019_02693 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/12); total (27/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACTGCAATGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCCGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGGCAGCAGTCAGCATTTCTG > KT2440/2839160‑2839416 | aCTGCAATGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGtt > 4:173805/1‑135 (MQ=255) aaTGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCt < 5:328466/134‑1 (MQ=255) ggCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGTCTTAAAGCTCATc > 6:27153/1‑135 (MQ=255) ggCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATc > 2:146354/1‑135 (MQ=255) ggCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATc > 4:174109/1‑135 (MQ=255) ggCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATc > 2:181261/1‑135 (MQ=255) ctcctTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGAc > 7:53256/1‑135 (MQ=255) ctcctTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGAc > 3:66148/1‑135 (MQ=255) cctTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACgg > 1:277757/1‑135 (MQ=255) gTGCTGATGTCATGGGAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCATTTATAATATAATAATCTGTCTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGAt < 3:192716/133‑1 (MQ=255) tGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATACGGGGACGGGATCTTTGa > 6:316624/1‑135 (MQ=255) aTGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATc > 6:114353/1‑135 (MQ=255) aTGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTATGAt > 3:104656/1‑134 (MQ=255) tGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGAGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATcc > 7:42483/1‑135 (MQ=255) tCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTTTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGAt > 6:52501/1‑131 (MQ=255) tCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGAt > 4:178921/1‑131 (MQ=255) tgtgtgGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTc > 4:251313/1‑97 (MQ=255) tgtgtgGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTc < 3:251313/97‑1 (MQ=255) tgtgtgGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGCTTCGTCCTGCa > 2:137543/1‑135 (MQ=255) tgtgtgGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGATTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCa > 4:269391/1‑135 (MQ=255) gtgGCTGAGATTTTTTCGAATCGACTGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAAc < 7:305969/134‑1 (MQ=255) ggCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCt > 1:206370/1‑135 (MQ=255) ggCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCt > 2:263850/1‑135 (MQ=255) cTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCg > 5:59990/1‑135 (MQ=255) cTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCATGCAACCCTCg > 6:297681/1‑135 (MQ=255) aTTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCaa < 3:173805/135‑1 (MQ=255) gaatcgaatGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGc < 8:51912/134‑1 (MQ=255) aatcgaatGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCaa < 2:158896/135‑1 (MQ=255) ttATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGaaa < 6:12277/135‑1 (MQ=255) tataAAATAAAAATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAAc < 6:237143/135‑1 (MQ=255) taaaataaaaATCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATACAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACAt > 5:132297/1‑135 (MQ=255) aaaTCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTtctc > 4:60693/1‑133 (MQ=255) aTCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCTAAACATCTTCTCTgcg > 6:19115/1‑133 (MQ=255) aTCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAg > 2:39873/1‑135 (MQ=255) aTCTGACTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAg > 3:240202/1‑135 (MQ=255) cTTATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGgca < 5:176733/132‑1 (MQ=255) tATCTCTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGGcagcag > 8:64018/1‑134 (MQ=255) tctcTATTTGAAAGTCTGTGTTGGCTTCAAGCTCATCCTGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGGCAGCAGt < 4:104656/133‑1 (MQ=255) aaaGTCTGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGGCAGCAGTCAGCATTTCTg < 3:178921/134‑1 (MQ=255) | ACTGCAATGACATGGGGCGAACCTCCTTGGTGCTGATGTCATGGCAGTAATGTGTGGCTGAGATTTTTTCGAATCGAATGCCGGGCCCAGTTATAAAATAAAAATCTGACTTATCTCTATTTGAAAGTCCGTGTTGGCTTCAAGCTCATCCGGGGACGGGATCTTTGATCCAGATTCGTCCTGCAACCCTCGGGCAATAGGGGCAAAGAGGTTTTCTATGCGAAACATCTTCTCTGAGGCAGCAGTCAGCATTTCTG > KT2440/2839160‑2839416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |