| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,858,207 | A→G | 19.9% | F78S (TTC→TCC) | nicP_5 ← | Porin‑like protein NicP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,858,207 | 0 | A | G | 19.9% | 40.2 / 3.5 | 20 | F78S (TTC→TCC) | nicP_5 | Porin‑like protein NicP |
| Reads supporting (aligned to +/- strand): ref base A (8/8); new base G (2/2); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGCCAGCGCCGTGCGGCCAAAGTCGTCCGCCGGGCGCCCGTCGTCGTGAACAGGCAGCAATTGCGTGCCGGCGGTGCCCTTGCCGCCATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCAACGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGG > KT2440/2858078‑2858335 | cccGCCAGCGCGGGGCGGCAAATGTCGTCCGCCGGGCGCCCGTCGGCGTGAACTGGCAGAAATTGCGTGCGGGCGGTGCCCTTGCCGCCTTCGAGTTTGATGCTTTAAAGGCCCAGCACGCAAACGCCTAAAcc < 4:134213/134‑1 (MQ=255)cccGCCAGCGCCGTGCGGCCAAAGTCGTCCGCCGGGCGCCCGTCGTCGTGAACAGGCAGCAATTGCGTGCCGGCGGTGCCCTTGCCGCCATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCAACGCCGAAAcc < 8:218331/134‑1 (MQ=255) cgtcCGCCGGGCGCCGGGCGTCGTGAACAGGCAGCAATTGCGGGCCGGCGGTGCCCTTGCCGCCATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCACCGCCGAAACCGACCACGCCGGGGGTGTAGCCGGAc < 8:265596/134‑1 (MQ=255) cAGGCAGCAATTGCGTGCCGGCGGTGCCCTTGCCGCCATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCAACGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTc > 8:140264/1‑134 (MQ=255) ttGCCGCCATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCAACGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTg > 7:247886/1‑135 (MQ=255) gccATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCAACGCCGGAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCgt > 1:52456/1‑135 (MQ=255) gccATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCAACGCCGGAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCgt > 1:57599/1‑135 (MQ=255) gccATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCAACGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCgt > 4:100113/1‑135 (MQ=255) gTTTGATGCTGAAAAGGCCCCGCACGGCCACGCCTAACCCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTcgc < 4:249021/135‑1 (MQ=255) gTTTGATGCTGAAAAGGCCCAGCACGTCACCGCCGAACCCCACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTcgc < 4:153622/135‑1 (MQ=255) gCTGTAAAGGCCCAGCACGTCAACGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCAt > 4:312251/1‑135 (MQ=255) ccccGCACGTCACCGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTa < 6:335421/135‑1 (MQ=255) cccacaaCGTCAAGCCCAAACCCCCCCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTa < 4:332333/129‑1 (MQ=255) cACGTCACCGCCCAAACCCACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCaa < 1:10184/135‑1 (MQ=255) cGTCAACGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACg > 8:289961/1‑135 (MQ=255) cGTCAACGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACg > 8:240599/1‑135 (MQ=255) cAACGCCGGAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCa < 2:57599/135‑1 (MQ=255) cAACGCCGGAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCa < 2:52456/135‑1 (MQ=255) aCGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGCAGTTACGCAACGTCAgg > 1:145940/1‑135 (MQ=255) aCGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAgg > 8:218039/1‑135 (MQ=255) | CCCGCCAGCGCCGTGCGGCCAAAGTCGTCCGCCGGGCGCCCGTCGTCGTGAACAGGCAGCAATTGCGTGCCGGCGGTGCCCTTGCCGCCATCGAGTTTGATGCTGTAAAGGCCCAGCACGTCAACGCCGAAACCGACCACGCCGGGGGTGTAGCCGGACTTGAAGTCGAAGATGAAACTTTGCGTCCATTCCTCGGCTTTGCCTTGGCTTGCCGTGCCCGTGTAGTCGCGGTTCATGTAGAAGTTACGCAACGTCAGG > KT2440/2858078‑2858335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |