Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,869,602 T→C 10.6% K277R (AAG→AGG)  CJ019_02729 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,869,6020TC10.6% 95.1 / 3.7 38K277R (AAG→AGG) CJ019_02729hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (16/17);  new base C (2/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

GTTGATCCTGCTTCACGTCGGGTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCTTCAAGGTCGTCACCACCCGCTCATGCGAAGGCGCGTTGCGCGCCGTGACGATGGCAAT  >  KT2440/2869468‑2869735
                                                                                                                                      |                                                                                                                                     
gTTGATCCTGCTTCACGTCGGGTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCt                                                                                                                                       <  4:188880/135‑1 (MQ=255)
      ccTGCTTCACGTCGGGTGCCTGAGGCCAGCCCACTCTGTTCGCAACCCCAAACGGTACATGCACAATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTTCGCAGGTCGTCGAAAAACACATGCTGATACAata                                                                                                                                 >  7:238550/1‑135 (MQ=255)
         gCTTCACGTCGGGTGCCTGAGGCGAGAGCACTCTGTTCGCCACCCCAATCTGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGAGCTGGTCGTCGAAAAACACATGCGGATTCAATATCg                                                                                                                              <  8:238550/135‑1 (MQ=255)
           ttCACGTCGGGTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCg                                                                                                                              <  5:58805/133‑1 (MQ=255)
           ttCACGTCGGGTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGa                                                                                                                             <  3:78935/134‑1 (MQ=255)
                  cGGGTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTTCATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACAccc                                                                                                                     >  4:262785/1‑135 (MQ=255)
                  cGGGTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCCTCAATATCGACAACAccc                                                                                                                     >  2:204070/1‑135 (MQ=255)
                   gggTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCt                                                                                                                    <  2:285071/135‑1 (MQ=255)
                    ggTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTg                                                                                                                   <  2:146248/135‑1 (MQ=255)
                           gAGGCGAGCGCACTCTGTTCGCCACCCCAAACCGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCGTCAATATCGACAACACCCTGGGCTTAt                                                                                                            >  5:157428/1‑135 (MQ=255)
                             ggCGAGCGCACTCTGTTCGCCACCACAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGTCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATcc                                                                                                          <  1:117744/135‑1 (MQ=255)
                                    gcACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATgccgg                                                                                                   >  2:96876/1‑134 (MQ=255)
                                    gcACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATgccgc                                                                                                   >  1:52309/1‑135 (MQ=255)
                                      aCTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATgccgc                                                                                                   >  5:338982/1‑133 (MQ=255)
                                               gCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGaaaaaa                                                                                         <  7:282642/134‑1 (MQ=255)
                                                     ccAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCa                                                                                  <  2:185988/135‑1 (MQ=255)
                                                         aCGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCg                                                                              >  7:226297/1‑135 (MQ=255)
                                                                aTGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATAc                                                                       >  8:109050/1‑135 (MQ=255)
                                                                 tGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCggg                                                                            >  1:117117/1‑129 (MQ=255)
                                                                 tGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCggg                                                                            <  2:117117/129‑1 (MQ=255)
                                                                  gCACCATCGGCAGGTTTCCCGCTGGAGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGc                                                                     <  2:108862/135‑1 (MQ=255)
                                                                    aCCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGcc                                                                    <  3:274378/134‑1 (MQ=255)
                                                                    aCCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCCTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGccc                                                                   <  1:204070/135‑1 (MQ=255)
                                                                        tCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGacaac                                                                                                                         >  4:97026/1‑77 (MQ=255)
                                                                        tCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGacaac                                                                                                                         <  3:97026/77‑1 (MQ=255)
                                                                        tCGGCAGGTTTACCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCact                                                               >  7:257141/1‑133 (MQ=255)
                                                                          ggCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATcc                                                             >  5:86977/1‑135 (MQ=255)
                                                                          ggCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATcc                                                             >  1:172793/1‑135 (MQ=255)
                                                                          ggCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCCTCAATATCGacaac                                                                                                                         <  7:287792/75‑1 (MQ=255)
                                                                          ggCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCCTCAATATCGacaac                                                                                                                         >  8:287792/1‑75 (MQ=255)
                                                                           gCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCt                                                            <  8:103413/135‑1 (MQ=255)
                                                                                   cccGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCTTCAAGgtc                                                    >  2:182917/1‑135 (MQ=255)
                                                                                   cccGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCTTCAAGgt                                                     <  7:109050/134‑1 (MQ=255)
                                                                                                  aGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCTTCAAGGTCGTCACCACCCGCTCa                                     >  6:290077/1‑135 (MQ=255)
                                                                                                            gcTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTCGGCTTATCCATGCCCCCTAGAAAAAAGCACTCATCAGGGGATACGCCCCAATCCTGCAAGGTCGTCACCACCCGCTCATCGGAAGgcg                           >  5:290427/1‑135 (MQ=255)
                                                                                                                   gtcgAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCTTCAAGGTCGTCACCACCCGCTCATGCGAAGGCGCGTTgcg                    >  4:178378/1‑135 (MQ=255)
                                                                                                                                tGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCTTCAATGTCGTCACCACACGCTCATGAGAAGGCGCGTTGCGCGCCGTGACGATg       <  2:214909/135‑1 (MQ=255)
                                                                                                                                     cTTCAATATCGATAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCTTCAAGGTCGTCACCACCCGCTCATGCGAAGGCGCGTTGCGCGCCGTGACGATGGCAAt  <  7:330684/135‑1 (MQ=255)
                                                                                                                                     cTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCTCCAATCCTTCAAGGTCGTCACCACCCGCTCATGCGAAGGCGCGTTGCGCGCCGTGACGATGGCAAt  <  3:324212/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GTTGATCCTGCTTCACGTCGGGTGCCTGAGGCGAGCGCACTCTGTTCGCCACCCCAAACGGTACATGCACCATCGGCAGGTTTCCCGCTGGCGATTGCAGGTGCGTGCGCTGGTCGTCGAAAAACACATGCGGCTTCAATATCGACAACACCCTGGGCTTATCCATGCCGCCTAGAAAAAAGCACTCATCCGGGGATACGCCCCAATCCTTCAAGGTCGTCACCACCCGCTCATGCGAAGGCGCGTTGCGCGCCGTGACGATGGCAAT  >  KT2440/2869468‑2869735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑