| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,061,793 | G→A | 100% | S592N (AGC→AAC) | qedA_2 → | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,061,793 | 0 | G | A | 100.0% | 82.3 / ‑6.8 | 28 | S592N (AGC→AAC) | qedA_2 | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (19/8); minor base C (0/1); total (19/9) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTA > KT2440/3061661‑3061916 | tttCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg < 1:254050/135‑1 (MQ=255)tttCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg < 7:138873/135‑1 (MQ=255) tAGTCTCCGGCTGGGGCGGCGCGGGGCCGCGGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc < 4:1532/135‑1 (MQ=255) tcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa < 3:116672/135‑1 (MQ=255) ggatGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCACACGGGTCAAGGACTTCAACAAGGGCGGCATGCTCTTGACCTTCCAGTTGCACAAGCAGTTGCAGCAAACGGCCCACGTCAAGCCATAAAgc > 8:115202/4‑135 (MQ=255) ggCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGCAGTGGCCAAACGGGTCAAGGCCGTCAACCAGGGCGGCATGCTCTGGACCTTCAACTTGCCCAAGCAGTTGCAGCAAACCGCAAACGTCAAGCCATAACgc > 4:157221/1‑135 (MQ=255) ggCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa < 3:94334/132‑1 (MQ=255) ggCTGGGGCGGCGCGGTGCCGCTGGGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCCAGCCATAACgc > 5:117715/1‑135 (MQ=255) ggcggcGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgcctgcct < 5:337142/135‑1 (MQ=255) cggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTAc > 6:159182/1‑135 (MQ=255) ggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTGAACCAGGGCGGCCTGGGCTGGCCCTTCAAGTTGCCCAAGCAGTTTCAGCCAACGGCAAAAGTCCAGCCATAACGCCTGcctcct > 4:286052/1‑135 (MQ=255) gCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg < 3:233676/135‑1 (MQ=255) gCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGACTGCCTACTGCCGCTtgctg < 2:23792/135‑1 (MQ=255) cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATc > 5:118143/1‑135 (MQ=255) aaGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTg > 6:233271/1‑134 (MQ=255) aGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTgg > 3:37319/1‑134 (MQ=255) ggTCAAGCACTTCACCCCGGGCGGCATGCTCTGGACCTTCATGTTCCCCACGCTGTGGCAGCAAAGGGCAACGGTCACGCAAAAACGCCGGCCTACTGCCGGTGGCTGCCGCTGTTGGGTGCCACGCGGCCctac < 4:226404/135‑1 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACg > 7:1633/1‑133 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACg > 2:5057/1‑133 (MQ=255) cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCg > 2:247822/1‑135 (MQ=255) gCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGTAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCa < 3:150565/135‑1 (MQ=255) aaGTTCCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCAAAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGGGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCg < 4:54856/134‑1 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGCTCGGTGGCAAGCGGCCCTACTACCAAATGCCGAGAGCCCCGCTGCCAACGATGCATTCGcc > 2:19409/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGCGCCCCGCAGCCAACCATGCATTCGcc > 4:226883/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCGACTACCAAATGACGAGAGCCCCGCTGCCAACGAAGCATTCGcc > 8:213627/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCGACTACCAAAAGAAGAGACCCCCGCAGCCACCCAAGCCTTCGcc > 6:12950/1‑135 (MQ=255) aaGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAcgc > 4:85436/1‑135 (MQ=255) cagcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCGACTACCAAATGACGAGAGCCCCGCTGCCAACGCTGCATTCGCCCGGCACGCCGGGcttc > 7:328448/1‑134 (MQ=255) agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGcttct > 7:336101/1‑134 (MQ=255) agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCGGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCACCGATGCATTCGCCCGGCACGCGGGGcttctt > 6:276464/1‑135 (MQ=255) gcaaacggcaaacgTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTa > 8:224369/1‑135 (MQ=255) | TTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTA > KT2440/3061661‑3061916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |