Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,179,891 C→T 9.2% A150A (GCC→GCT)  actP_2 → Cation/acetate symporter ActP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,179,8910CT 9.2% 151.1 / 5.4 56A150A (GCC→GCT) actP_2Cation/acetate symporter ActP
Reads supporting (aligned to +/- strand):  ref base C (23/27);  new base T (2/3);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

TCATCGCTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCTGATGATGCTGTA  >  KT2440/3179758‑3180022
                                                                                                                                     |                                                                                                                                   
tCATCGCTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCCCCGCGACCAAGGCAAGGTGAGCCCCATGGCCGCCa                                                                                                                                    >  8:128712/1‑135 (MQ=255)
  aTCGCTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATc                                                                                                                                  >  6:96408/1‑135 (MQ=255)
  aTCGCTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACATCGTATCGCCTCTACCAAGGCAAGGTGCGCACCATGTCCGCCATc                                                                                                                                  >  2:53839/1‑135 (MQ=255)
   tCGCTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCg                                                                                                                                 >  1:274829/1‑135 (MQ=255)
     gCTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGc                                                                                                                               >  2:283345/1‑135 (MQ=255)
     gCTTTCTTCGCGGGCTGGCCGGTGATGCTGCTCTTGATGACCGAGCGGCTGCGCAACCTGGCCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCCACCAACGCAACGTGCGCCACATGGCCGCCATCGGc                                                                                                                               >  2:47249/1‑135 (MQ=255)
      cTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCCCCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCTCCATGGCCGCCATCGGCt                                                                                                                              >  1:5146/1‑135 (MQ=255)
      cTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCt                                                                                                                              >  7:33646/1‑135 (MQ=255)
           ttcGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCAACTCGTATCGCCTCGACCAAGGCAAGGTTCGCACCATTGCCGCCATCGGCTCGcgg                                                                                                                         >  1:7626/1‑133 (MQ=255)
           ttcGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCAACTCGTATCGCCTCGACCAAGGCAACGTGAGCACCATGGCCGCCATCGGCTCGcag                                                                                                                         >  1:1860/1‑133 (MQ=255)
              gcgGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTg                                                                                                                         <  4:104181/132‑1 (MQ=255)
              gcgGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTg                                                                                                                         <  1:283345/132‑1 (MQ=255)
              gcgGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGa                                                                                                                        <  6:62684/133‑1 (MQ=255)
                gggCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCgt                                                                                                                    <  3:43567/135‑1 (MQ=255)
                gggCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCgt                                                                                                                    <  2:714/135‑1 (MQ=255)
                gggCTGGCCGGTGTTGCTGCTGGTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCgt                                                                                                                    <  8:158705/135‑1 (MQ=255)
                                 tgctgTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCtgg                                                                                                   <  3:14181/135‑1 (MQ=255)
                                   ctgTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCtggtg                                                                                                 >  1:45154/1‑135 (MQ=255)
                                        gatgaCCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCTATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGgc                                                                                               >  2:187197/1‑132 (MQ=255)
                                        gatgaCCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCTATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGgc                                                                                               >  2:192589/1‑132 (MQ=255)
                                          tgaCCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGGGGTGTGTTTCTAACTGGTGGCGCAgc                                                                                          >  4:78443/1‑134 (MQ=255)
                                                   ggCTGCGCAACCTGGGCCGCTTCCCCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCGCGCGGACCGTGGTGTGTTTCTAACTGGTGGCGCAGAGGGTCgggg                                                                                 >  7:250221/1‑135 (MQ=255)
                                                   ggCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTc                                                                                                                             <  7:21428/91‑1 (MQ=255)
                                                   ggCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTc                                                                                                                             >  8:21428/1‑91 (MQ=255)
                                                   ggCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCgggg                                                                                 >  7:196818/1‑135 (MQ=255)
                                                                          aCCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCgt                                                                                                                    <  2:22476/77‑1 (MQ=255)
                                                                          aCCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCgt                                                                                                                    >  1:22476/1‑77 (MQ=255)
                                                                          aCCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCt                                                           <  2:249283/134‑1 (MQ=255)
                                                                          aCCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCt                                                           <  5:96408/134‑1 (MQ=255)
                                                                          aCCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGGGGCGCAGATGGGCGGGGCCGGGCAGTTGATCAGGTTGc                                                            <  3:300026/133‑1 (MQ=255)
                                                                                gCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCg                                                      <  1:4288/133‑1 (MQ=255)
                                                                                 cagATATCACCTCGTATCGCCGCGCCCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCTGGCAGTTGATCAGGTTGCTGTTCGGc                                                    <  1:288692/132‑1 (MQ=255)
                                                                                  cGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGGCGGGGCCGGGCAGTTGATGAGGTTGCTGTTCGGCCt                                                  >  5:12665/1‑135 (MQ=255)
                                                                                   gATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTg                                                 <  3:123957/135‑1 (MQ=255)
                                                                                   gATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTg                                                 <  8:196818/135‑1 (MQ=255)
                                                                                   gATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCAGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTGCGGCCTg                                                 >  6:324608/1‑135 (MQ=255)
                                                                                     tagccccTCGTCTCGCCTCCACCACGGATAGGGGCGCACCATGGCCGCGATCGGCTCGCGGTCCGGGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGGTGCTGTTCGGCCTGGa                                               <  2:170735/130‑1 (MQ=255)
                                                                                         aCCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTAc                                           >  3:167329/1‑135 (MQ=255)
                                                                                               tATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATc                                     <  4:185162/135‑1 (MQ=255)
                                                                                                   gCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACAGCGCgg                                 >  7:134759/1‑135 (MQ=255)
                                                                                                    ccTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCggt                                <  1:15249/135‑1 (MQ=255)
                                                                                                        gACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTCGTGTGTTTCTACCTGGTGGCCCAGATGGTCCGGGCCCGGCACTTGCTCAGCTTGCTTATCGGCCTGGACTACCCCATCGCGGTGGTc                            >  6:255100/1‑135 (MQ=255)
                                                                                                            caggcacggTGCGCACCATGGCAGCCCGCGCCGCGCTGACCGTGCGGTGTGTCAACCTGGTGGCGCAGCTGGGCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCAtcg                        <  6:212079/134‑1 (MQ=255)
                                                                                                            aaggcaaggAGCGCAACATCGCCGCCATCGGATAGCTGACCGTGGTTTGTTTCTACCTTCTGGCGCATATGGTCTGGGCCGGGCAGTTGATCAGGTTGCTTTTCGGACTGGACTACCACATCTCGGTGGTCAtcg                        <  6:207999/135‑1 (MQ=255)
                                                                                                                caaggTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCgg                    <  3:13241/135‑1 (MQ=255)
                                                                                                                  aggTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTg                  <  1:47249/135‑1 (MQ=255)
                                                                                                                      gcgcACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCAGATCGCGGTGGTCATCGTCGGTGCGCt              <  8:13953/135‑1 (MQ=255)
                                                                                                                       cgcACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCt              <  2:300824/134‑1 (MQ=255)
                                                                                                                       cgcACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCt              >  1:300824/1‑134 (MQ=255)
                                                                                                                        gcACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCtga            <  2:5146/135‑1 (MQ=255)
                                                                                                                          aCCATGGCCGCTATCGGCTCGCTGACCGTGGTGTGTTTCTAGCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCtgatg          <  1:187197/135‑1 (MQ=255)
                                                                                                                          aCCATGGCCGCTATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCtgatg          <  1:192589/135‑1 (MQ=255)
                                                                                                                          aCCATGGCCGCCATCGGCTCGCTGTCCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCtgatg          <  5:255100/135‑1 (MQ=255)
                                                                                                                          aCCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGGCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGACTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCtgatg          <  8:862/135‑1 (MQ=255)
                                                                                                                          aCCATGGCCGCCATCGGCTCGCTGACAGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGGTGATCACGTTGCTGTTCGGCCTGCACTACCACATCCCGGTGGTCATCGTCGGTGAGCtgatg          >  8:146521/1‑135 (MQ=255)
                                                                                                                             aTGGCCGCTATCGTCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGGGCAGATGGTTGTGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCTGatgatg       <  5:194067/135‑1 (MQ=39)
                                                                                                                                 ccgccATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCTGATGATGCTGt   >  6:127067/1‑135 (MQ=255)
                                                                                                                                  cgccATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCTGATGATGCTGTa  >  4:100142/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                   
TCATCGCTTTCTTCGCGGGCTGGCCGGTGTTGCTGCTGTTGATGACCGAGCGGCTGCGCAACCTGGGCCGCTTCACCTTTGCCGATATCACCTCGTATCGCCTCGACCAAGGCAAGGTGCGCACCATGGCCGCCATCGGCTCGCTGACCGTGGTGTGTTTCTACCTGGTGGCGCAGATGGTCGGGGCCGGGCAGTTGATCAGGTTGCTGTTCGGCCTGGACTACCACATCGCGGTGGTCATCGTCGGTGCGCTGATGATGCTGTA  >  KT2440/3179758‑3180022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑