Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,210,887 A→C 41.5% Q206P (CAG→CCG)  oprJ_2 → Outer membrane protein OprJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,210,8870AC41.5% 11.3 / 3.3 30Q206P (CAG→CCG) oprJ_2Outer membrane protein OprJ
Reads supporting (aligned to +/- strand):  ref base A (7/9);  new base C (2/11);  total (9/21)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGCACGCATCGCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGGTGCTGTTGCTGG  >  KT2440/3210759‑3211021
                                                                                                                                |                                                                                                                                      
tGCCGCACGCATCGCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCCGGCGCGGCCCCGTCAGCCCCTGGCCCGCCGCGAGGCTTCCCTGGCGTTGGTCGGCCCgcggcg                                                                                                                                  >  3:249752/1‑135 (MQ=255)
  ccgcccgcATCGCCCTGCTCGCCGCGGTGCGCCCGGCCTTCCGGGCCTTGGCCGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGgcgcg                                                                                                                                <  7:267283/135‑1 (MQ=255)
              cccTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCCCCGCCCGcacgg                                                                                                                    >  2:4373/1‑135 (MQ=255)
                     cGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGCCCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCccgccccgcccccgc                                                                                                             >  2:106798/1‑130 (MQ=255)
                           ggTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGACGCTGCCCCGTCAGACCCTGGCCAGCCGCGAGGAGTCCCTGGCGTTGGTCGGCACGCGGCGGCCCGCCGGCCCCGCCCCCGCGCgggc                                                                                                       >  2:334870/1‑134 (MQ=255)
                                cccgggCCAACCGGGCCTGGCCCGCCGCCGCGCGGCGCCCGGCGCGCCCCCGCCACACCCGGCCCCGCCGCGAGGATCCCCTGGCGTTGGGCGGCCCGCGGCGCGCCGCCGCCACGGCCACGGCGCTGGaccacc                                                                                                  <  4:160820/131‑1 (MQ=255)
                                            tGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCCGCCCCGCCCCGGCGCGGGGCCCCCAAGAGGCGCggg                                                                                      >  7:27058/1‑135 (MQ=255)
                                                  tGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCCGCCCGGCCCCCGCGCTGGGCCCCCAAGAGGCGCGgggggggg                                                                                >  6:174290/1‑131 (MQ=255)
                                                    gccGGCGCCGCGGGGCGCCAGGCGCTCACCCGTCCCACCCGGCCCAGCCGCTAGGATTCCCTGGGGTGGGGCGGCCAGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTc                                                                              <  3:319038/133‑1 (MQ=255)
                                                    gccGGCGCCGAGCGCCGCCTGGCGCTGACCCGGCAGACCCTGGCCAGCCGGGAGGATTCCCTGGCGTGGGGCGGCCTGCGGCGCGCCGCCGGCAGGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTc                                                                              <  8:49704/133‑1 (MQ=255)
                                                      cGGCGCCGCGCGCCGCCCGGCGCTCACCCCTCCGACCCTGCCCCGCGGCGTGTTTTCCCGGGCGGTGGTCGGCCCGCGGCGCGCCGCGGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGa                                                                            <  5:129933/135‑1 (MQ=255)
                                                               ggggcgCCAGGCGCTGACCCGACAGACCCGGGCCGGCCGCGAGGAGTCCGTGGCGGTGGCCGGCCCGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCg                                                                   <  7:182302/133‑1 (MQ=255)
                                                               gcggcgCCCGGCGCGCACCCGCCAGACCCTGGCCCGCCGCGAGGATCCCCTGGCGTGGGGCGGCCCGCGGCGCGCCGCCGCCCCGCCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCg                                                                   <  7:168048/135‑1 (MQ=255)
                                                                     cccggCGCTGCCCCGTCAGACCCTGGCCCGCCGCGAGGATCCCCTGGCGTGGGGCGGCCAGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGa                                                             <  6:248093/132‑1 (MQ=255)
                                                                       gggCGCGGACCCGGCTGACCCGGGCCAGCGGGGTGGTTCCCCGGGGGTGGGGGGGCCCGCGGGGCCCCGCCGGCCCGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGc                                                           <  2:108147/134‑1 (MQ=255)
                                                                                cccGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCCGCACCGCCCCGGCGCGGGCCCCCCAAGAGGCGCGGGGGCTGGTGGAGCCGGCCCGTGCCGAGCTCGAAGTCa                                                  >  6:136137/1‑135 (MQ=255)
                                                                                        ccccGGGCCAGCCGCGAGGATCCCCTGGCGTGGGGCGCCCCGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTgcgc                                          <  7:13638/134‑1 (MQ=255)
                                                                                         ccgtGGCCCGCCGCGAGGATGGCCTGGCGGTGGTCGGCCAGCGGCTGGCGGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGcc                                         <  7:262409/132‑1 (MQ=255)
                                                                                         cccTGCCCCGCTGCGAGGATCCCCTGGCGGTGGAGGGCCAGGGGCGGGCCGCCGGCAGGCCCACGGCGCTGGACCACCATGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGcc                                         <  7:257992/135‑1 (MQ=255)
                                                                                              gCCAGCCGCGAGGATGCCCTGGCGTGGGGCGGCCCGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAg                                    <  7:73314/135‑1 (MQ=255)
                                                                                              gCCAGCCGCGAGGATCCCCTGGCGTTGGGCGGCCCGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAg                                    <  7:81271/135‑1 (MQ=255)
                                                                                              cccAGCCGCGAGTATACCCGGCGGTTGGCCGGCCCGGGGCGCGCGGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGGCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAg                                    <  2:118629/134‑1 (MQ=255)
                                                                                                cAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGGCACGGCCACGGCGCGGGCCCACCCAGAGGCGCTGGGGCTGGTCGAGCCGTCCCGTGCCGCGCTCGAAGTCACCCTGCGCCAACAgcg                                  >  5:44187/1‑135 (MQ=255)
                                                                                                         ggctgcccTGGCGTGGGGCGGCCCGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTa                         <  7:194103/130‑1 (MQ=255)
                                                                                                         ggATTCCCTGGCGTGGGGCGGCCCGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTa                         <  7:156310/135‑1 (MQ=255)
                                                                                                         ggATTCCCTGGCGTGGGGCGGCCAGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTa                         <  4:273926/135‑1 (MQ=255)
                                                                                                                cTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGGCCCGGCCCCGGCGCGGGACCCCCAAGAGGCGCGGGGGCTGGTCGAGCCGTCCCGTGCCGCGCTCGCAGTCACCCTGCGCCAACAGCGCCCGGCCGACCAcgcg                  >  7:256478/1‑135 (MQ=255)
                                                                                                                              ccAGCGGCGCGCGGCGGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGGTGCTGTTGCt    <  1:297104/135‑1 (MQ=255)
                                                                                                                              ccAGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGGTGCTGTTGCt    <  8:72534/135‑1 (MQ=255)
                                                                                                                              ccAGCGGCGCGCCGCCGGCACGCCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGGTGCTGTTGCt    <  8:67032/135‑1 (MQ=255)
                                                                                                                                aGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGGTGCTGTTGCTgg  <  4:8506/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                                      
TGCCGCACGCATCGCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGGTGCTGTTGCTGG  >  KT2440/3210759‑3211021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑