Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,231,966 T→G 19.0% F14V (TTC→GTC)  lgoT_2 → putative L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,231,9660TG19.0% 62.0 / 2.7 33F14V (TTC→GTC) lgoT_2putative L‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base T (9/17);  new base G (5/2);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAACGGCTCGCTGCGTCAGGGAGGGGCGCAGCATCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCGTCGCAGATGGGCATCCTGTTGTCGGCGTTTTCCATTG  >  KT2440/3231834‑3232095
                                                                                                                                    |                                                                                                                                 
tGAACGGCTCGCTGCGTCAGGGAGGGGCGCAGCATCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGACCCCAATGAAGAAGTACCAGCGCATTACCGTCGTGGTc                                                                                                                                 >  3:213459/1‑135 (MQ=255)
          gCTGCGTCAGGGAGGGGCGCAGCATCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCCCATTACCGTCGTGGtgctgctgcggc                                                                                                                       >  7:181375/1‑132 (MQ=255)
               gttagggagggGCGCAGCATCATCAAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGGTCCTGCTGCTGATCGGc                                                                                                                  >  5:186805/4‑135 (MQ=255)
                tCAGGGAGGGGCGCAGCATCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCa                                                                                                                 <  1:49439/135‑1 (MQ=255)
                         ggCGCAGCATCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAAc                                                                                                         <  5:23942/134‑1 (MQ=255)
                           cgcAGCATCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTAc                                                                                                      <  3:162892/135‑1 (MQ=255)
                            gcagcaTCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCAGGCTTCAGATAGGCCTCGTCAACTAcc                                                                                                     >  8:99528/1‑135 (MQ=255)
                              agcaTCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTg                                                                                                   >  2:177751/1‑135 (MQ=255)
                              agcaTCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGGTGCTGCTGCTGATCGGCATCGTCAACTAccag                                                                                                   >  2:139435/1‑133 (MQ=255)
                              agcaTCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAAACCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTACTTCTGCTGATCGGGATCGTCAACTACCt                                                                                                    >  4:43297/1‑134 (MQ=255)
                                        aGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGt                                                                                          <  1:177751/134‑1 (MQ=255)
                                             tCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCAGCTGCAGAAAGGCATAGTCAACTACCTGGCCCGTACTGCGCTg                                                                                    >  2:17721/1‑135 (MQ=255)
                                             tCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTACTGCATCTGTTCCGGATGGGCAACATCCTCGCCCGGAGTgcggag                                                                                    >  5:86849/1‑132 (MQ=255)
                                               gTCAAGGCGCTGACAGCGAGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTc                                                                                  <  8:108712/135‑1 (MQ=255)
                                                                   gCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCtccatcc                                                              <  8:37917/135‑1 (MQ=255)
                                                                   gCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCtccatcc                                                              <  1:17721/135‑1 (MQ=255)
                                                                      acaaTAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGa                                                           <  1:317234/135‑1 (MQ=255)
                                                                          taataataaAGGTAATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGa                                                       <  8:288614/135‑1 (MQ=255)
                                                                                         tcaCAAGAACCCAATGAAGAAGTACCAGAGCATTACCGTCGTGGTCCTGCTGCTGATCGTCATCGTCAAGTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGcc                                         <  7:84748/134‑1 (MQ=255)
                                                                                          cacaTGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCg                                       <  1:176986/135‑1 (MQ=255)
                                                                                          cacaTGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGGTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCg                                       <  6:186805/135‑1 (MQ=255)
                                                                                           acaTGAACCCAATGAAGAAGTACCATCTCATTAACGTCTTGTTACATATGCTGATCGGCATCGTCAACTACCTTGCCCGTAGTGCGCTGTCGATCGCCAACCCCGCCATCCAGAATCACATGATGATCAGCCCGt                                      >  6:223341/1‑135 (MQ=255)
                                                                                           acaTGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGCCCGTAGTGCGCTGTCGATCGCCCACCCCGCCATCCAGAAAGACATGATGATCAGCCCGt                                      >  8:39926/1‑135 (MQ=255)
                                                                                              tGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCGTCGc                                   <  1:98674/135‑1 (MQ=255)
                                                                                                  cccAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCGTCGCAGAt                               >  7:314994/1‑135 (MQ=255)
                                                                                                      aTGAAGAAGGACCAGCGCATTACCGTCGTGTTCCTGCGGCTGATCGGCATCGTCAACTACCTGGACCGTATGGCGCTGTCGATCGCCAACCCCTCCATCCATAAAGACATGATGATCAGCCCGTCGCAGATGGGc                           <  7:112009/135‑1 (MQ=255)
                                                                                                          agaagTACCAGCGCATTACCGTCGTGGTCCTGCTGCTGATCGGCATCGTCAACTACCTGGCCCGTAGTGCGCTGTCGATCGCCCACACCACCCTCCAGAAAGACAAGATGATCAGCCCGTCGCCGATGGGCATcc                       >  2:259533/1‑135 (MQ=255)
                                                                                                                   aGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCGTCGCAGATGGGCATCCTGTTGTCgg              <  8:314994/135‑1 (MQ=255)
                                                                                                                         ttACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCGTCGCAGATGGGCATCCTGTTGTCGGCGtttt        <  1:259533/135‑1 (MQ=255)
                                                                                                                         ttACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCGTCGCAGATGGGCATCCTGTTGTCGGCGtttt        <  1:154277/135‑1 (MQ=255)
                                                                                                                         ttACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATAATCAGCCCGTCGCAGATGGGCATCCTGTTGTCGGCGtttt        <  1:62243/135‑1 (MQ=255)
                                                                                                                             cgtcgtGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCGTCGCAGATGGGCATCCTGTTGTCGGCGTTTTCCa     <  3:43297/134‑1 (MQ=255)
                                                                                                                                cgtGTTACTGGTGCTGATAGGCATCGTCAAATACCTGGACCGTAGTGGGCTGTGGATAGCCAACACCGCCAAACAGAAAGACATGATTATCAGCCCGTCGCAGATGGGCCTACTGTTGTTGGGGTTTTTCATTg  >  2:62243/1‑134 (MQ=255)
                                                                                                                                    |                                                                                                                                 
TGAACGGCTCGCTGCGTCAGGGAGGGGCGCAGCATCATCGAGGGATCGTCAAGGCGCTGACAGCGCGGCAACAATAATAATAAAGGTCATCACATGAACCCAATGAAGAAGTACCAGCGCATTACCGTCGTGTTCCTGCTGCTGATCGGCATCGTCAACTACCTGGACCGTAGTGCGCTGTCGATCGCCAACACCTCCATCCAGAAAGACATGATGATCAGCCCGTCGCAGATGGGCATCCTGTTGTCGGCGTTTTCCATTG  >  KT2440/3231834‑3232095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑