| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,285,727 | A→C | 52.4% | T652P (ACT→CCT) | tamB → | Translocation and assembly module subunit TamB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,285,727 | 0 | A | C | 52.4% | 6.6 / 15.1 | 20 | T652P (ACT→CCT) | tamB | Translocation and assembly module subunit TamB |
| Reads supporting (aligned to +/- strand): ref base A (2/7); new base C (9/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-02 | |||||||||||
GCAGGCCAACGGCAAGGGCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCAGGAGTGGCAGTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTGG > KT2440/3285600‑3285854 | gCAGGCCAACGGCAAGGGCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAATCTCGACCTTGTCCTCGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGacggtccacaggcc > 1:53156/1‑134 (MQ=255)gCAGGCCAACGGCAAGGGCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGacgggccactggcc > 1:260606/1‑134 (MQ=255)gCAGGCCAACGGCAAGGGCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGacgggccacaggcc > 2:2514/1‑134 (MQ=255)gCAGGCCAACGGCAAGGGCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGGCCCGGGCCg > 4:333307/1‑135 (MQ=255) aCGGCAAGGGCGACATGCGCCAACCGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGGGACGGGCGCGGCCGACTGGCCACTGGCCGAATCCAgg < 5:91013/135‑1 (MQ=255) aCGGCAAGGGCGACATGCGCCAACAGGCCTTGACCCGGGCGCGGGACGGCCCGCAGCGCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAgg < 2:177927/135‑1 (MQ=255) cAAGGGCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCCCGGGCCGCATACACGCCgg > 6:172311/1‑135 (MQ=255) cAAGGGCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGCTGAACAAAGGTGACTGGCGCGGCCGACGGGGCCCTGGGCGCATCCCGGCCgc > 4:272944/1‑134 (MQ=255) aaGGGCGACATTCGCCAACAGGCCTTGACCCTGGGGCTGGACGGCCCGCAGCTCACGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGt < 8:332703/135‑1 (MQ=255) acggCGACCTTCGCCAACAGGCCTTGACCGTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGCCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTg < 3:134195/133‑1 (MQ=255) gCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGGCCCTGGCCGCATCCACGCCGgggggc > 2:162832/1‑133 (MQ=255) gCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGCCCCTGGCCCAATCCCCGCCGGTgg > 6:336025/1‑133 (MQ=255) aTTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCAGGAg < 3:138305/135‑1 (MQ=255) gCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCCCAGGCCGAAACCCGCCCCGGGGGCACGCGTGGCAGTTTCAg > 4:67210/1‑135 (MQ=255) gCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGGCCCAGGGCGCCTCCAGCCCGGGGGGGCCGACTGGCAGTTGGAg > 6:294771/1‑135 (MQ=255) cgcTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGGCACTGGCCGCATACACGCCGGGGGGCCGCAGTTGGCGTTGGAAGcgcctgcccgcc > 8:236930/1‑135 (MQ=255) cgcTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGCCCCAGGCCGCAAACAGGCCCGTGGCCAGGAGTGGCAGTTGCAGGcgccggcccccc > 2:98277/1‑130 (MQ=255) cGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGGCACAGGCCGCCTCCAGGCCGGTGGCCAGGCGTGGGGGTTTCGGGCGCCAGCCCCCCGGCAGCGGCggg > 5:320034/1‑135 (MQ=255) tCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGAGTGGGCCCGGGGCGAATACAGGCCGGTGGCGCGGCGTGGCAGTTGCAGGCCCCGGCACGCCGGCAGCGGCTggcccccg > 5:87857/1‑131 (MQ=255) ctgcgtGGCAATTTGAACAAGGGTCCCGGGCGCGGCCGACTGGCCAGTGGCCGAAGCCCGGCGGGTGGCCTGGAGTGGCAGTTGCAGGCGCCGGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCggg < 1:174795/130‑1 (MQ=255) tGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCTGGAGTGGCAGTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGgc < 6:326243/135‑1 (MQ=255) caacaacGGTGAGGGGCGCGGCCGAGTGGCACCTGGCCGAATCCCGGCGGGGGGCCAGGAGTGGCAGTTGCTGGCGCCGGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGgc < 6:299273/133‑1 (MQ=255) gggCGCGGCCGACTGCCACCTGGCCGAATCCCGGCGGGTGGCCTGGAGGGGCAGTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCa < 4:323509/134‑1 (MQ=255) actggcaagtggccGAATCCTGGCGGGTGGCCAGGAGGGGCTGTTGCTGGGGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTgg < 2:53156/135‑1 (MQ=255) | GCAGGCCAACGGCAAGGGCGACATTCGCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCAGGAGTGGCAGTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTGG > KT2440/3285600‑3285854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |