Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,308,725	A→C	33.7%	L164R (CTG→CGG)	CJ019_03110 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,308,725	0	A	C	33.7%	31.7 / 6.1	47	L164R (CTG→CGG)	CJ019_03110	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/11);  new base C (3/14);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAGGGAAGGT  >  KT2440/3308606‑3308852                                                                                                                        |                                                                                                                                ccTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCACCGCCCCCGGCCGCGGCCGAAAc                                                                                                                  >  4:184138/1‑135 (MQ=255)       ggCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACa                                                                                                            >  1:9248/1‑135 (MQ=255)             ggggttgtgCCACCCCGGCGCCGCGCGCGGCGCCCACCAACTTGCCGGCCAAAGCCACCACCTCCACCCGCCCGCCGTGGCGCCGCGCGCCCCGCACCCACCCCCCCCGGCCGCGGCCGAAACGCGACAGGgcgc                                                                                                      <  4:60652/132‑1 (MQ=255)             ggTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGgcgc                                                                                                      <  8:97921/135‑1 (MQ=255)             ggTGTTGTCGCAACCAGGCGCCGCGCGCGGCGCCCAGCAACATGCCGCCCCAAGCCACCAACTCCACCCGCCCCCGGTTGCGCCGCGCGCCCAGCAGCCAACGCCCCTGGCGGCGGCCGAAACGCGACAGGgcgc                                                                                                      <  3:328266/135‑1 (MQ=255)                  tgtcgCAACCCGGCGCCGCGCGCGGCGCCCACAAACATGCCGGCCCACGCCACAAACTGCCCCCCCCAGCCGTGGCGCCGCGCGCCCACCACCCAACGCCCCAGGCCGCGGCCTAAACGCGACAGGGCGCCCGtt                                                                                                 <  5:246847/135‑1 (MQ=255)                     tgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTAc                                                                                               >  3:144661/1‑134 (MQ=255)                     tgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCACCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGtttc                                                                                               >  1:226709/1‑132 (MQ=255)                      gCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGCAACGCGACAGGGCGCCCGTTACg                                                                                              >  7:208040/1‑134 (MQ=255)                       cAACCAGGCGCCGCGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGt                                                                                             <  7:34924/134‑1 (MQ=255)                        aacctcgcggcgTGCGGGGGGCCCTGCATCTTGCCGGCCCAAGGCACCAACTGCACCCGCCTGCCGTTGCGCCGCGCGCCCCGCCGCCAACGCCCCAGGCCGGGGCCGTAACGCGACAGGGCGCCCGTTACGTa                                                                                            <  7:263712/128‑1 (MQ=255)                         aCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAg                                                                                          <  7:150104/135‑1 (MQ=255)                         aCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAg                                                                                          <  3:148703/135‑1 (MQ=255)                           cAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAg                                                                                          >  3:36908/1‑133 (MQ=255)                                    cgcgcGGCGCCCACCCACATGCCGGCCCCAGGCACCACCGCCACCCCCCAGCCGGGGCGCCGCGCGCCCACCCCCCAACGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATg                                                                               <  1:157294/134‑1 (MQ=255)                                    agggcggcgCCCAGCAACATGCCGGCCCAAGGCACCAACTGCCCCCGCCCGCGGTTGCGCCGCTCGCCCAGCAGCCACCGCCCCCGCCCGCGGCCCAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATg                                                                               <  8:186533/132‑1 (MQ=255)                                    aGCGCGGCGCCCAGCAACATGCCGGCCAATGGCACCAACTGCCCCCGCCCGCGGTTGCGCCGCGCGCCCACCAGCCACCGCCCCCGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATg                                                                               <  8:208040/135‑1 (MQ=255)                                     gcggGGCGCCCAGCAACAGGCCGCCCCAAGCCACCAACAGCCCCCGCCAGCCGTTGCGCCGCGCGCCCAGCAGCCACCGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATgg                                                                              <  7:328621/135‑1 (MQ=255)                                       gcggcgCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATgg                                                                              >  7:224729/1‑133 (MQ=255)                                          gcgcCCCCCAACATGCCGGCCCAAGGCACCAACTGCCCCCGCCAGCCGTGCCCCCGCGCCCCCAGCCGCCACCGCCCCTGGCCGCGGCCTAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAgg                                                                         <  8:176954/135‑1 (MQ=255)                                          gcgcCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCACCGCCCCCGGCCGCGGCAGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAgg                                                                         <  7:57330/135‑1 (MQ=255)                                          gcgcCCACCAACATGCCGGCCCAAGCCACCAACTCCACCCGCCCGCCGTGGCGCCGCTCCCCCACCACCCACCCCCCCCGCCCGCGCCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAgg                                                                         <  6:317838/135‑1 (MQ=255)                                          gcgcCCACCAACAGGCCGGCCCACGCCACCAACTCCCCCCGCCAGCCGTTCCGCCGCGCCCCCAGCAGCCACCGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAgg                                                                         <  6:279563/135‑1 (MQ=255)                                            ccccAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCCTAGCGCCGCTCGCCCAGCAGCCACCGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGcc                                                                       >  2:75424/2‑135 (MQ=255)                                              ccAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCAt                                                                     >  4:28833/1‑135 (MQ=255)                                                aGCAACATGCCGGCCCACGGCACCACCTCCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTc                                                                   <  8:81423/135‑1 (MQ=255)                                                aGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTc                                                                   <  4:270182/135‑1 (MQ=255)                                                aGCAAAATGCCGGCCCATGGCACCACCTCCCCCCCCCAGCCGTTCCGCCGCGCGCCCAGCTCCCAACCCCCCCGCCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTc                                                                   <  2:98042/135‑1 (MQ=255)                                                   aaCATGCCGGCCCCAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCCGGCCGCGGCCGAAACGCGACCGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGCCCATTcc                                                                  >  5:211337/1‑132 (MQ=255)                                                          cgccccACGGCACCACCTCCACCCGCCAGCCGTTGCGCCGCCCCCCCAGCACCAAACGCCCCAGGCCGCGGCCTAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCa                                                         <  1:69269/132‑1 (MQ=255)                                                          cGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCa                                                         >  8:229061/1‑135 (MQ=255)                                                           ggCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCTCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGCACCCAGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGACATTCACCTGTTcccc                                                        >  4:20657/1‑133 (MQ=255)                                                           ggCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCCGCCAACGCCCCCGGCCGCGGCCGAACCGCGCCTGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCAc                                                        >  5:249702/1‑135 (MQ=255)                                                           gccccACGCCACCACCTGCCCCCGCCCGCGGTGGGGGCGCGCGCCCCCCAGCCAACGCCCCCGGGCGCGCCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCAc                                                        <  6:86396/133‑1 (MQ=255)                                                               cAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGAcc                                                    >  1:300074/1‑135 (MQ=255)                                                               cAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGAcc                                                    >  3:310186/1‑135 (MQ=255)                                                               aaaGCCACCAAGGGCACCCCCCCGCGGTGGCGCCGCCCCCCACGCACCCACCCCCCCCGCCGGCGGCCCAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGAcc                                                    <  2:289584/134‑1 (MQ=255)                                                                          ctccACCCGCCCGGCGTGGCGCCGGTCCCCAAGCCCCAAACGCCCCTGGCCGCGGCCGAACCGCGACCGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCGCCTGTTCCACGACCGCATTGATCAt                                         <  2:248431/132‑1 (MQ=255)                                                                                     ggCCGGTGCGCCGCGCGCCCAGCCGCCACCGCCCCCGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCa                              <  8:104298/134‑1 (MQ=255)                                                                                     aGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCa                              >  4:90043/1‑135 (MQ=255)                                                                                     aGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCa                              >  4:209217/1‑135 (MQ=255)                                                                                          ttGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTg                         >  3:170729/1‑135 (MQ=255)                                                                                          tggcgcCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTg                         <  4:170664/133‑1 (MQ=255)                                                                                               ccgcccgcCCCCCAGCCACCGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGgccgc                    <  1:221014/135‑1 (MQ=255)                                                                                                  cgcGCCCCCCAGCCCCCGCCCCCGGCCGCGCCCGAACCGCGACAGGGCGCCCGGTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCaa                 <  1:322169/133‑1 (MQ=255)                                                                                                             gcCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAgg        >  1:193873/1‑133 (MQ=255)                                                                                                                 ccGCCCCCGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAGGGAAGGt  <  4:261706/134‑1 (MQ=255)                                                                                                                        |                                                                                                                                CCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAGGGAAGGT  >  KT2440/3308606‑3308852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑