| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,449,038 | G→C | 9.3% | intergenic (‑91/+2) | acsA_3 ← / ← eco | Acetyl‑coenzyme A synthetase/Ecotin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,449,038 | 0 | G | C | 9.3% | 137.2 / 4.2 | 43 | intergenic (‑91/+2) | acsA_3/eco | Acetyl‑coenzyme A synthetase/Ecotin |
| Reads supporting (aligned to +/- strand): ref base G (18/21); new base C (2/2); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
TCGATCTGCGTTGTGGACGGTCGCCAGAGCACATCGTTCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGCTGTTGTAGCGCAGCAGGAAGCC > KT2440/3448907‑3449159 | tCGATCTGCGTTGTGGACGGTCGCCAGAGCACATCGTTCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCct < 3:134484/135‑1 (MQ=255)tCGATCTGCGTTGTGGACGGTCGCCAGAGCACATCGATCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGCCCCTGCAGAGAAAGCGGTCCTGCct > 2:263331/1‑135 (MQ=255)tCGATATGCGTTGTGGACGGTCGCCAGAGCACATCGTTCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCct < 3:167990/135‑1 (MQ=255) cGATCTGCGTTGTGGACGGTCGCCAGAGCACATCGTTCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCcta > 7:222315/1‑135 (MQ=255) tgtgGACGGTCGCCAGAGCACATCGTTCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTa < 6:146268/135‑1 (MQ=255) agagCACATCGTTCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCCGCCGCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATc < 6:128861/135‑1 (MQ=255) gagCACATCGTTCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATcc > 6:233469/1‑135 (MQ=255) ttCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTACGATGCCg < 2:131800/135‑1 (MQ=255) tCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCct < 1:331071/98‑1 (MQ=255) tCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCct > 2:331071/1‑98 (MQ=255) tCATGACGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTAGTCTACTTTAGCCTTCTGCACATCCTGCGATGCAGa > 7:43515/1‑135 (MQ=255) aTGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGAc < 7:187460/134‑1 (MQ=255) gCCTAAGCTCCTTTCCCGGAACAAAACCACGCCGAGGGCAGGATCCGCCTCGTGTTGCGAAGCGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACcac < 5:336742/135‑1 (MQ=255) ccGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCATGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACcaca > 5:262388/1‑135 (MQ=255) tACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGACTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGCCCACACGCGGTAGCGCa > 5:279725/1‑135 (MQ=255) cgcaaCAAACGCCGGCGCAGGGCAGGATCAGCCGCGTGTTGCGACGAGGCTGACCCTGCAGAGAAAGCGGGCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACtt < 5:47072/132‑1 (MQ=255) ggAACAAAAGCCGGCTCAGGGCAGGATCAGCCTCGTGGTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTc < 7:286984/135‑1 (MQ=255) taaCAAAAGCAGGCGCAGGGCAGGATCAGCCTGGTGTTGCTAAGAGGCTGACCCTGCTGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCTACCACACGCGTTAGCGCACTTCg < 1:263331/134‑1 (MQ=255) aaacGCCGGCTCCGGGCAGGCTCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACAt < 3:1047/135‑1 (MQ=255) aaaGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCct < 5:14503/71‑1 (MQ=255) aaaGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCct > 6:14503/1‑71 (MQ=255) aaaGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACttt < 7:28154/81‑1 (MQ=255) aaaGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACttt > 8:28154/1‑81 (MQ=255) gCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGATAAAGCGGGCCTGCCTACTCTACTTTCGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCtt < 2:568/135‑1 (MQ=255) tgggCAGGATCAGCCTCGTGTTGCTAAGAGGCTGACCCTGCAGAGAAAGCTGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACAt < 5:111822/134‑1 (MQ=255) cAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATAcaccaccac > 7:78907/1‑135 (MQ=255) cAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATAcaccaccac > 1:50422/1‑135 (MQ=255) tCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTa < 7:162442/94‑1 (MQ=255) tCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTa > 8:162442/1‑94 (MQ=255) tCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTCCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGAcc < 1:169484/84‑1 (MQ=255) tCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTCCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGAcc > 2:169484/1‑84 (MQ=255) tCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTCCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGAcc > 2:165483/1‑84 (MQ=255) tCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTCCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGAcc < 1:165483/84‑1 (MQ=255) ttGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTg > 3:116084/1‑135 (MQ=255) tGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGc > 3:52209/1‑135 (MQ=255) gAAGAGGCTGACCCTGCAGAGAAACCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCGGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGCtg < 8:222315/134‑1 (MQ=255) agagGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGCtgtt > 3:2739/1‑134 (MQ=255) ggCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCGTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGCTGTTGTa < 5:233469/134‑1 (MQ=255) gACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGCTGTTGTAGCgca > 7:87484/1‑135 (MQ=255) aCCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGCTGTTGTAGCgcag < 3:18893/135‑1 (MQ=255) agaAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGc > 6:182363/1‑51 (MQ=255) agaAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGc < 5:182363/51‑1 (MQ=255) agaAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGCTGTTGTAGCGCAGCAGGAAGcc > 8:271443/1‑135 (MQ=255) | TCGATCTGCGTTGTGGACGGTCGCCAGAGCACATCGTTCATGCCGAATCTCCTTTACCGGAACAAAAGCAGGCTCAGGGCAGGATCAGCCTCGTGTTGCGAAGAGGCTGACCCTGCAGAGAAAGCGGTCCTGCCTACTCTACTTTAGCCTTCTGCACATCCTGCGATGCCGACCACACGCGGTAGCGCACTTCGACATCCTTGGGCACATACACCACCACCGGCAGCTTGCTGTTGTAGCGCAGCAGGAAGCC > KT2440/3448907‑3449159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |