Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,653,349 A→C 19.7% V148G (GTG→GGG)  mtnB_2 ← Methylthioribulose‑1‑phosphate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,653,3490AC19.7% 64.1 / 3.0 53V148G (GTG→GGG) mtnB_2Methylthioribulose‑1‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (14/27);  new base C (4/8);  total (18/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TCCACGCTGACACCTCCGGTCAGCAGCCCGTGGTTGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTTGCTCGTCCAGGTCCAGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGTGCGCGGTGGATGGCGCT  >  KT2440/3653220‑3653482
                                                                                                                                 |                                                                                                                                     
tCCCCGCTGACACCTCCGGTCAGCAGCCCGTGGTTGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTTGCGCGTGCGGGTCCCGCGCGACGGCTTCGTAGTCGTGGTGAAacacccct                                                                                                                                  >  6:21462/1‑131 (MQ=255)
  cccgcgGACCCCTCCGGTCAGCAGCCCGTGGTTGCGCAAAATACGAATGTGGCTGTCGCCCATATGGGCCACCAGCGGTGCTTGCTCGTCCCGGCCCAGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCt                                                                                                                                <  4:62838/133‑1 (MQ=255)
  cccGCTGACACCTCCGGGCAGCAGCCCGTGGTGGCGCAAAATACGAATGTTGCGGGCGCCCAGATGGGCCACCAGCCGGGCTTGCGCGGCCCGGCCCCGGGCGACGCCTTCGTAGTCGTGGAAACCCACGCGGCt                                                                                                                                <  4:67467/133‑1 (MQ=255)
  cACGCTGACCCCTCCGGCCAGCCGCCCGTGGTTGCGCAAAATAAGATTGTGGCGGGCGCCCAGTGTGGCCCCCAGCGGTGCTTGCGCGGCCCGGCCCCGGGCGACGCCTGCGTAGGCGGGGTAAACCCCGCGGCt                                                                                                                                <  6:293994/135‑1 (MQ=255)
   aCGCTGACACCTCCGGTCAGCAGCCCGTGGTTGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTTGCTCGTCCAGGTCCAGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTg                                                                                                                               <  8:15588/135‑1 (MQ=255)
       tGACACCTCCGGTCAGCAGCCCGTGGTTGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTTGCTGGTCCAGGTCCAGGGCGACGCCGTCGTAGTCCTGGTTAAACAAGCGGCTggggt                                                                                                                           >  1:180121/1‑131 (MQ=255)
         acacCTCCGGTCAGCAGCCCGTGGTTGCTCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCGTGCTCGGCCAGGTCCAGGGCGCCGCCTTCGTAGTAGTGGTAAACAACGCGGCGGttgtag                                                                                                                         >  4:20740/1‑135 (MQ=255)
           cccTCGGGTCCGCTGCCGGTGTTGGCGCAAAATAATATTGTTGGTGGCGCCCAGTTGGGGCACCAGCCGTGCTTGCTCGTCCTGTTCCCGGGCGACGCGTTCGTAGGGGTGGTAACCCACGCGGCTGTAGTAGGc                                                                                                                       <  8:322990/134‑1 (MQ=255)
                      gcagcCCGTGGTTGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTGGCTCGTCCAGGTCCAGGGCGACGCCGTCGTACTCGTGGTAAACCCCCCCGCTGTAGTACGCCAAGGCCTGCt                                                                                                            >  3:197124/1‑135 (MQ=255)
                        agcCCGTGGTTGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGGTTGCTCGTCCAGGTCCAGGGCGCCGCCGGCGTATTCGGGGGAAACCCCGCGGCGGGTGTATGCCAAGGCggggggg                                                                                                          >  3:142/1‑129 (MQ=255)
                        agcCCGTGGTTGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTTGCTCGTCCAGGTCCAGGGCGCCGCCGTCGTAGTCGTGGTAAACCACGCGGCGGTAGTAGGCCAAGGCGTGCTgg                                                                                                          >  4:316400/1‑135 (MQ=255)
                                  tGCGCAAAATAATAAGGTTGCGGGCGCCCACTTTGGCCCCCCGCCTTGCTTGCTCGGCCCGGGCCCGGGCGACGCCTTCGAAGTCGTGGAAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGgca                                                                                                <  4:12275/135‑1 (MQ=255)
                                  tGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGGTTGCTCGTCCAGGTCCACGGCGCCGCCTTCGTAGTCGTGGTAAACCACCGCGCGGGTGTAGGCCAAGGCGTGCGGGGAGATCGgca                                                                                                >  5:236854/1‑135 (MQ=255)
                                  tGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTTGCTCGGCCAGGGCCAGGGCGACGCCGTCGTAGTCGTGGGAAACCACCCGGCGGGAGGAGGCCAAGGCGTGGTGGGAGGTCGgcc                                                                                                >  5:241217/1‑134 (MQ=255)
                                            aaGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTTGCTCGTGCAGGTCCAGGGCGCCGCCTTCGTAGTAGTGGTAAACCCCGCGGCGGTAGGAGGCCCAGGCGTGCGGGGGGCTCGGCAGCACCCCCTc                                                                                      >  6:219845/1‑135 (MQ=255)
                                                        gTCGCCCAGATTGGCCACCAGCCGTGCTTGCTCGTCCAGGTCCAGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGGTGGGAGCTCGGCAGCAACCCGTCGCGCGGGGCggg                                                                          >  1:245015/1‑134 (MQ=255)
                                                                     gCCACCAGCCGTGCTTGCTCGTCCAGGGCCAGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGcc                                                              <  7:7530/134‑1 (MQ=255)
                                                                     gCCACCAGCCGTGCTTGCGCGCCCAGGGCCAGGGCGACGCCTTCGTCGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGcc                                                              <  3:171962/134‑1 (MQ=255)
                                                                     gCCACCAGCCGTGCTGGCTCGCCCCGGGCCCGGGCGCCGCCTTCGTAGGCGTGGTAAACCCCGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGcc                                                              <  3:43837/134‑1 (MQ=255)
                                                                     gCCACCAGCCGGGCTTGGTCGCCCAGGTCCTGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGcc                                                              <  4:197124/134‑1 (MQ=255)
                                                                     gCCACCAGCCGGGCTTGCTCGTCCAGGGCCCGGGCGACGCCTTCGTCGTCGTGTTAACCCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGcc                                                              <  3:232313/134‑1 (MQ=255)
                                                                        accaGCCGTGCTTGCTCGTCCAGGTCCAGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGGGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACCGCCGCGCCGTcg                                                          >  4:293892/1‑135 (MQ=255)
                                                                          caGCCGTGCTTGCTCGACCCGGTCCCGGCCGACGCCTTCGTAGTCGTGGAAAACCCCGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTcgcg                                                        <  6:747/135‑1 (MQ=255)
                                                                           aGCGGTGATTTCTCGTCCAGCTCGAGGGATACGCGTTTGAAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCTAGGAGTGCTGGGAGATCTGCATCAACCCTTCGCGCTTGGCGGACACAGCCGCGCCGTCGCGa                                                       <  4:45629/135‑1 (MQ=255)
                                                                              ggttcttgctGGTCCAGGGCCAGGGCGAGGCCTTCGTTGTGGTGGTAAACAACGCGGCTGTAGTAGGACAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTa                                                    <  8:298481/131‑1 (MQ=255)
                                                                              ggtgcgtgcTCTCCCCGGTCCCGGGCGACGCGTTCGAAGGCGTGTAAACCCCCGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTa                                                    <  5:219845/134‑1 (MQ=255)
                                                                              ggggctggcgCGTCCCGGGCCCGGGCGCCGCCTTGTTCGTGGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTa                                                    <  8:120624/132‑1 (MQ=255)
                                                                              cGTGCTTGCTCGTCCAGGTCCAGGGCGACGCCGTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCCAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGTCACAGCCGCGCCGGCGCGAGTa                                                    >  6:107263/1‑135 (MQ=255)
                                                                                   ttgctCGTCCAGGCCCAGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGgt                                               <  2:27640/135‑1 (MQ=255)
                                                                                   tgggcggtcccggccccgGGCGAGGCCTTGGTCGGGGTGGTAAACCCCGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGgt                                               <  5:308102/129‑1 (MQ=255)
                                                                                        cgcccagttcctggGCGACGACTTCTTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTgca                                          <  7:205761/132‑1 (MQ=255)
                                                                                          tccaggtccaggGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTgca                                          <  4:169683/133‑1 (MQ=255)
                                                                                          tccaggtccaggGCGACGCCTGCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTgcagc                                        <  2:70490/135‑1 (MQ=255)
                                                                                          gccaggtccaggGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTgcagc                                        <  4:218135/134‑1 (MQ=255)
                                                                                             aggtccaggGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGCCCCAGCCGCGCCGTCGCGAGGATGGGTGTGCAGcaca                                     >  7:241922/1‑135 (MQ=255)
                                                                                                tccaggGCGACGCCTTCGTCGGCGGGGTAACCCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAg                                    <  1:207996/133‑1 (MQ=255)
                                                                                                      gCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTgag                             >  2:19747/1‑134 (MQ=255)
                                                                                                        gACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTgaga                            >  3:132804/1‑133 (MQ=255)
                                                                                                            ccTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTcgc                                                         <  4:238412/100‑1 (MQ=255)
                                                                                                            ccTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTcgc                                                         >  3:238412/1‑100 (MQ=255)
                                                                                                             cTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTTTGGGTGTGCAGCACCGCCTTGAGATCAGGcc                     >  8:329798/1‑135 (MQ=255)
                                                                                                               tCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGt                   <  2:106717/135‑1 (MQ=255)
                                                                                                                 gttgtggTGGTAAACCCCGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGt                   <  5:333487/127‑1 (MQ=255)
                                                                                                                 gtggTCGGGGCAACCCCCGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGTGTGTGCAGCACAGCCTTGAGATCAGGCCGTgc                 <  5:196962/132‑1 (MQ=255)
                                                                                                                      cggggTAACCCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGTGCGCGGt            <  6:241217/132‑1 (MQ=255)
                                                                                                                      cGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGTGCGCgg             <  6:96948/134‑1 (MQ=255)
                                                                                                                      cGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGTGCGCGGt            <  6:236854/135‑1 (MQ=255)
                                                                                                                      cGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGTGCGCGGt            <  5:161656/135‑1 (MQ=255)
                                                                                                                          gTAAACCCCGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTc                                                                                      >  6:42799/1‑57 (MQ=255)
                                                                                                                          gTAAACCCCGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTc                                                                                      <  5:42799/57‑1 (MQ=255)
                                                                                                                           tAAACCACGCGGCTGTAGTAGGCGAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACATCCTTGAGATCAGGCCGTGCGCGGTGGATg       <  5:140755/135‑1 (MQ=255)
                                                                                                                           tAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGTGCGCGGTGGATg       <  8:318562/135‑1 (MQ=255)
                                                                                                                             aaCCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCCGCACAGCCTTGAGATCAGGCCGTGCGCGGTGGATGgc     >  5:268758/1‑135 (MQ=255)
                                                                                                                                cACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGTGCGCGGTGGATGGCGCt  <  1:178297/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                     
TCCACGCTGACACCTCCGGTCAGCAGCCCGTGGTTGCGCAAAATAAGAATGTTGCTGTCGCCCAGATTGGCCACCAGCCGTGCTTGCTCGTCCAGGTCCAGGGCGACGCCTTCGTAGTCGTGGTAAACCACGCGGCTGTAGTAGGCCAAGGCGTGCTGGGAGATCGGCAGCAACCCGTCGCGCTGGGCGGACACAGCCGCGCCGTCGCGAGTATGGGTGTGCAGCACAGCCTTGAGATCAGGCCGTGCGCGGTGGATGGCGCT  >  KT2440/3653220‑3653482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑