Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,761,329	A→C	44.3%	E96A (GAG→GCG)	CJ019_03574 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,761,329	0	A	C	44.3%	30.0 / 5.8	42	E96A (GAG→GCG)	CJ019_03574	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/10);  new base C (15/4);  total (27/15)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTG  >  KT2440/3761199‑3761457                                                                                                                                   |                                                                                                                                 ggCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCg                                                                                                                              <  3:39013/135‑1 (MQ=255)  gCATGGTCCAAGTTGAGCAAGTCTGCAGGGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCGGCTGACCCTGCTCGCCGGCGAGCCgg                                                                                                                             <  6:50227/135‑1 (MQ=255)  gCATGGTCCAAGTAGAGCAAGACTTCAGCGATCCGACCCTGGCTGCCGGGGGTGACTGCGTGATCGCAAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCTAGCCgg                                                                                                                             <  6:183182/135‑1 (MQ=255)  gCATGGGCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCgg                                                                                                                             <  3:23852/135‑1 (MQ=255)   cATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTAGGCCTGCTGACCCTGCTCGCCCGCCCGCCggg                                                                                                                            >  1:11384/1‑135 (MQ=255)     tGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCCGCCCGCCGGGCa                                                                                                                          >  7:116078/1‑135 (MQ=255)                  gCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACcca                                                                                                              <  4:23278/134‑1 (MQ=255)                    aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCCGGCCGCCCCGTAAcccacc                                                                                                           >  3:84602/1‑135 (MQ=255)                    aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCCGCCCCGTACcccacc                                                                                                           >  8:188913/1‑135 (MQ=255)                    aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACccaac                                                                                                            >  6:140822/1‑134 (MQ=255)                    aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGAACCTTCTCCCCGGCGAGCCCGGCCGCCCCCTACccacc                                                                                                            >  8:121420/1‑132 (MQ=255)                    aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTCTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACccaacc                                                                                                           >  4:58086/1‑135 (MQ=255)                          tCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCGAGCCGGCGCGCCCGGCCGCCCCCTACCCcacccaccgc                                                                                                     >  1:122487/1‑133 (MQ=255)                              cGATGCGACGCTGGGTGCGGGCGGTGTCTGCGTGAGCGCCAGGGTGTTCAGCCCCAGAGTGCTGGCCGTGTTTTTCGGCCGGGTGACCGGGCGCGCCGGCGTGCCGGGCAGCCCCTTACCCAACCAACCTCCGcc                                                                                                 <  6:221418/135‑1 (MQ=255)                              cGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTTCTGACCCTGCACGCCGGCGCGCCGGGCCTCCCCGTACCCCACCCACCTCCGcc                                                                                                 >  7:159861/1‑135 (MQ=255)                              cGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCCGGCGCCCCCCTACCCCCCCAACCACcccc                                                                                                 >  1:328841/1‑135 (MQ=255)                              cGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGcc                                                                                                 <  2:111020/135‑1 (MQ=255)                                             tGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCGGGCCGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAagca                                                                                  >  2:75348/1‑135 (MQ=255)                                              gCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCGGGGAGCCCCTTACCCAACCCACCTCCGCCACGCCTGCGCAagcag                                                                                 >  2:91411/1‑135 (MQ=255)                                              gCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCGGGCCGCCCCTTACCCAACCCACCTCCGCCCCGCCAGCCCAcgccg                                                                                 >  4:196216/1‑133 (MQ=255)                                              gCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCACGCCGGCGAGCCGGGCCCCCCCGTACCCCACCACCCTCCGCCAACCCTGccccatccg                                                                                 >  8:7140/1‑129 (MQ=255)                                                                     ctgCCTGTGCAGCGCCAGAATGGTGGCGGTGTTTTTCTGCCTGCTGACGGTGGTCGCCGGCTCGCCGGGCAGCCCGTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCcg                                                          <  4:198993/133‑1 (MQ=255)                                                                     cAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCcg                                                          <  8:250649/135‑1 (MQ=255)                                                                       gCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCGGGCAGCCCCTTACCCAACCCACCTCCGCCACGCCAGCCCAAGCAGCGCTGGCCCCTGGTCAATCCCcgcg                                                        >  2:178011/1‑135 (MQ=255)                                                                         cTGTGCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGcc                                                      <  6:108934/135‑1 (MQ=255)                                                                           gTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTTCTCGCCGGCGAGCCGGGCAGCCCCGTACCCAACCAACCCCCGCCACGCCTGCCCAAGCCGCGCTGGCCCCTGGTCAATCCCCCCGCctc                                                    >  5:93194/1‑135 (MQ=255)                                                                               aGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCGCGCCGGCGCGCCGGGCCGCCCCGTACCCACCCACCCACCGCCCCGCCTGCCCCAGCACCCCGGGCCCCAGGGCAAACCCCCCGCCTCGccc                                                >  6:180718/1‑135 (MQ=255)                                                                                gcgcCACCGTGCTGGCCTTCTTTTGGGGCCTGCTCACGGTGGGCGGCGGCGCGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcg                                               <  2:11384/135‑1 (MQ=255)                                                                                  gcCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgct                                             >  4:97211/1‑135 (MQ=255)                                                                                         tgctgGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCGGGCAGCCCCGTACCCAACCAACCTCCGCCACGCCTGCCCCAGCAGCGCTGGGCCCTGGTCCATCCCCGCGCCTCGCCCgcgtcgcttc                                      >  5:66629/1‑135 (MQ=255)                                                                                              gCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCGGGCCGCCCCTTACCCCCCCAACCTCCGCCCCGCCTGCCCAAGCAGCGCTGGCCCCGGGTCAATCCCCGCGCCTCGCCCGCGTCGCTGCTcgcg                                 >  6:144694/1‑135 (MQ=255)                                                                                               ccGTCTTTTTCGGCCTGCTGACCCTGCGCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTcgc                                  <  7:5028/133‑1 (MQ=255)                                                                                                 gTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCCGGCCGCCCCGTTCCCCACCAACCGCCCCCCCGCATGCCCAAGCAGCGCAGGCCACTGGTCCATCCCCCCGCCGCTCCCGCGTCGCTTCTCGCGTTc                              >  8:148069/1‑135 (MQ=255)                                                                                                  tgttttgCGGCGGGCTCACCCTGCGCGCCGGCGCGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCt                             <  5:139365/133‑1 (MQ=255)                                                                                                      tttCGGCCTGCTGACCCTGCTCGCCGGCGCGCCGGGCCGCCCCGTACCCCACCCACCTCCGCCCCGCCTGCCCCAGCCGCGCTGGCCCCTGGTCAATCCCCCCGCCCCTCCCGCTTCGCTTCTCGCGTTCGGACa                         >  6:139138/1‑135 (MQ=255)                                                                                                      tttCCGCCTGCTGACCCTGCTCGCCGGCCGGCCCGGCAGCCCCTTACCCCACCCACCACCCCCCCCCCAGCCCAAGCACCGCGGGCCCCGGGTCAAACCCCCCCCCACACCCGCTTAGCCTCTCGCGTTcgtaca                         >  8:181134/1‑130 (MQ=255)                                                                                                          ggCCTGCTGACCCGGCGCGCCGGCGCGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCg                      <  1:476/134‑1 (MQ=255)                                                                                                                       ggCTCGCCGTCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTgc        <  8:116078/134‑1 (MQ=255)                                                                                                                            gCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCt   >  8:90300/1‑135 (MQ=255)                                                                                                                             ccGGCGAGCCGGGCCGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCCAGCCGCGCTGGCCACTGGTCAATCCCCGCGCCGCGCCCGCTTGGCGTCTCGCGGTCTGCCATCGCCACGGCCCCGTgccgccgg  >  3:214737/1‑133 (MQ=255)                                                                                                                             ccGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCACTCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTg  >  5:146886/1‑135 (MQ=255)                                                                                                                             ccGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTg  >  5:109657/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                 GGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGGCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTG  >  KT2440/3761199‑3761457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑