Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,850,110 A→C 16.3% F117V (TTC→GTC)  eamB_1 ← Cysteine/O‑acetylserine efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,850,1100AC16.3% 154.6 / 2.2 82F117V (TTC→GTC) eamB_1Cysteine/O‑acetylserine efflux protein
Reads supporting (aligned to +/- strand):  ref base A (37/29);  new base C (2/14);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 1.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCGCAGCACCGT  >  KT2440/3849981‑3850236
                                                                                                                                 |                                                                                                                              
cGCTGGGCAATTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAAcgccg                                                                                                                           <  7:77147/135‑1 (MQ=255)
cGCTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGTGGTTGACCCACTGGAAcgccg                                                                                                                           <  6:154934/135‑1 (MQ=255)
cGCTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAAcgccg                                                                                                                           <  5:220979/135‑1 (MQ=255)
 gCTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAAcgccgc                                                                                                                          >  1:252520/1‑135 (MQ=255)
  cTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGACcgccgcc                                                                                                                         >  3:96073/1‑135 (MQ=255)
  cTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAAcgccgcc                                                                                                                         >  8:95981/1‑135 (MQ=255)
  cTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAAcgccgcc                                                                                                                         >  5:299268/1‑135 (MQ=255)
  cTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAAcgccgcc                                                                                                                         >  5:130442/1‑135 (MQ=255)
     ggCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCa                                                                                                                      >  6:122593/1‑135 (MQ=255)
      gCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCAc                                                                                                                     >  8:337962/1‑135 (MQ=255)
        aaGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGGTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCAccc                                                                                                                   <  6:269326/135‑1 (MQ=255)
        aaGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCAccc                                                                                                                   >  6:35692/1‑135 (MQ=255)
        aaGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCAcc                                                                                                                    >  7:205954/1‑134 (MQ=255)
        aaGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCAcc                                                                                                                    >  8:172414/1‑134 (MQ=255)
        aaGTTGACCAAAGCCAACAAGGCCGCAATGACGAGCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCAccc                                                                                                                   <  5:46337/135‑1 (MQ=255)
          gTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCAcc                                                                                                                    >  2:254514/1‑132 (MQ=255)
                 acaGCGAACCAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCCAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGccc                                                                                                          <  7:273465/133‑1 (MQ=255)
                  aaGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGACGCCCa                                                                                                         >  7:76586/1‑135 (MQ=255)
                       aaCAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGt                                                                                                    <  5:122593/135‑1 (MQ=255)
                             gCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGGTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCgg                                                                                              <  2:287159/135‑1 (MQ=255)
                             gCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCgg                                                                                              <  5:35692/135‑1 (MQ=255)
                                       ccaTCCCGTGGGTTACATCGCTTTGCGGTGGGGTTTAGGGGGTAAGCGCCCCTACGGCAATCACCCACGCCTTGGGGTTCACCCACTGGACCGCCGCCGCACCCCAGACGCCCAGCGGTTTGCGGGCACTGGAcg                                                                                    <  6:57566/133‑1 (MQ=255)
                                         aTCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCCTCACCCAAGCCTTGGGGTTGACCCCCTGTAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGAcgcg                                                                                  >  2:194263/1‑135 (MQ=255)
                                                     aCATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGACCGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGCCGCGGCCGTGCCGccc                                                                      >  6:327524/1‑135 (MQ=255)
                                                     aCATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCCCCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGAcc                                                                      >  8:221734/1‑135 (MQ=255)
                                                           ccTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGGTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCg                                                                >  3:186798/1‑135 (MQ=255)
                                                                   tGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCCCGGGACGCGGCCGGGCCGACCGGCCCGGGAGTggg                                                        >  5:128141/1‑134 (MQ=255)
                                                                   tGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGc                                                        >  5:83168/1‑135 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCCTCAACCAAGCCTTGGGGGTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCCGCGGTTTGCGGGCACAGGACGCGGCCGGGCCGACCGGCCCGGAAggggagttga                                                   >  3:7050/1‑129 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGCCCCACTGGAACGCCGCCGCACCCCAGAAGCCCCGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGCAGTgggggggg                                                   >  8:134817/1‑129 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGCACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGCCGCGGCCGTGCCGCCCGGCCCGGCAGTggggctcg                                                   >  5:161799/1‑129 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAAcgccg                                                                                                                           <  7:243930/63‑1 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAAcgccg                                                                                                                           >  8:243930/1‑63 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGGGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTgg                                                         <  7:29820/129‑1 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCCCTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGctct                                                   >  7:41628/1‑135 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGCAGGGGCggacg                                                   >  6:206434/1‑131 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTgg                                                         >  8:29820/1‑129 (MQ=255)
                                                                        tgtAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATcg                                                   >  3:301242/1‑134 (MQ=255)
                                                                        tcaaGGTGGTAATCGCCCCTACCGCAAAAACCAAATCATATGGCTTGACACACTGGAACGCCGCCGCACCCCACAAGCCCAGCGCTTTGCGGGCACTGGACGCGGCCGTCCCGACCGGCCCGCAAGTGGCGATca                                                   >  4:105933/4‑134 (MQ=255)
                                                                           cgcgtgtAAGCCCCCCCCCCGCCATCCCCCCACCCTTGGGGTTCACCCACTGGACCGCCCCCGCCCCCCCGAACCCCCGCGGTTGGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTcc                                                <  1:73734/130‑1 (MQ=255)
                                                                             gtggtAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGTAGTGGCGATCATccag                                              >  8:258162/1‑135 (MQ=255)
                                                                                  aaTCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCTCTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTccaggccag                                         >  1:15139/1‑135 (MQ=255)
                                                                                  aaTCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGcc                                                                          >  7:219487/1‑102 (MQ=255)
                                                                                  aaTCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGcc                                                                          <  8:219487/102‑1 (MQ=255)
                                                                                  aaTCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTccaggccag                                         >  3:80425/1‑135 (MQ=255)
                                                                                  aaTCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAACTGGCGCTCGTacaggccag                                         >  2:168433/1‑135 (MQ=255)
                                                                                   agcGCCCCTCCCGCCACCACCCACGCCTGGGGGTGCACCCACGGCCCCGCCGCCCCCCCCCAGACCCCCCGCGGTTGGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTccaggccagg                                        <  4:186798/133‑1 (MQ=255)
                                                                                   accGCCCCTCCCGACATCACCCAAGCCTTGGGTTTCCCCCACTGGAACGCCGCCGCCCCCCACAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTccaggccag                                         <  1:254514/132‑1 (MQ=255)
                                                                                    gcGCCCCTCCCGCCATCACCCAAGCCTTGGGGTTGACCCACTGCAACGCCGCCGCACCCCAGAAGCCCCGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGt                                       <  1:112690/134‑1 (MQ=255)
                                                                                          cTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTaaa                                    >  3:140924/1‑132 (MQ=255)
                                                                                              cGCCATCACCCACGCCTTGGGGTTGCCACCCTGCCCGGCCGCCGCACCCCCGACGCCCCGCGGTTTGCGGGACCTGGACGCGGCCGTGCCGACCGGCCCGGAAGGGGCTATCTTCCAGGCCAGGTAAAGCAGATa                             <  4:28149/135‑1 (MQ=255)
                                                                                              cGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATa                             <  4:140924/135‑1 (MQ=255)
                                                                                                caaTCACCAAAGCCTTGGGGTTTACCCACTGCACCGCCGCCGCACCCCAGACCCCCAGCGGTTGGGGGCCACGGGCCGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAgg                           <  3:105933/133‑1 (MQ=255)
                                                                                                   tCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAggcgg                        <  7:266501/135‑1 (MQ=255)
                                                                                                   acACCCACGCCTTGGGGTTGCCCCACTGGACCGCCGCCGCACCCCAGAAGCCCAGGGGTTTGCGGGCAGTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAggcgg                        <  7:337962/134‑1 (MQ=255)
                                                                                                     aCCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGgcggcg                      >  6:15215/1‑135 (MQ=255)
                                                                                                      cccaccccTTGGGGTGGACCCACTGCAACGCCGCCGCCCCCCAGAAGCCCAGCGGTTTGCGGGCACGGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGgcgc                     <  4:301242/129‑1 (MQ=255)
                                                                                                        cacgcgttGGGGTTCACCCACGGCACCCCCGCCCCACCCCAGACGCCCCGCGGTTGGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCa                   <  1:677/129‑1 (MQ=255)
                                                                                                        cacgCCTTGGGGTTGACCCACTGCAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCa                   <  4:163536/132‑1 (MQ=255)
                                                                                                        cacgCCTTGGGGAGGACCCAGTGCAACGCCCCCGCACCCCAGACGCCCAGCGGTTTGCGGGCACTGCACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCa                   <  6:89905/132‑1 (MQ=255)
                                                                                                        cacgCCTGGGGGTTGACCCACTGGACCGCCGCCGCCCCCCCCACGCCCCGCGGTTTGCGGGCACGGGCCGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCa                   <  8:67217/132‑1 (MQ=255)
                                                                                                        caccccTTGGGGTTCACCCACGGGACCGCCGCCGCACCCCAGAAGCCCAGCGGTTGGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCa                   <  1:75111/131‑1 (MQ=255)
                                                                                                        cAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCa                   <  6:83168/135‑1 (MQ=255)
                                                                                                        cAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGGAAAGCAGATAGGCGGCGCCa                   >  4:271841/1‑135 (MQ=255)
                                                                                                        cAAGCCTGGGGGTTGACCCACTGGACCGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCa                   <  8:329018/135‑1 (MQ=255)
                                                                                                        aacgCCTTGGGGTTGACCCACTGCAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCa                   <  7:45626/132‑1 (MQ=255)
                                                                                                             cTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTa              >  2:116055/1‑135 (MQ=255)
                                                                                                              ttGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAATCAGATAAGCCGCGCCACCGTAg             >  4:322547/1‑135 (MQ=255)
                                                                                                               gggggTTGACCCACTGTACCGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGCCGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAgc            <  6:161799/134‑1 (MQ=255)
                                                                                                                  ggttcccccACTGTAACGCCGCCGCACCCCAGACGCCCCGCGGTTGGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgca         <  7:95981/129‑1 (MQ=255)
                                                                                                                  ggtggccccACTGCAACGCCGCCGCACCCCAGACGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgca         <  6:130442/129‑1 (MQ=255)
                                                                                                                  ggtgccccccCTGCACCGCCGCCCCACCCCCCCCGCCCCGCGGTTGGCGGGCACTGCCCGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgca         <  7:134817/129‑1 (MQ=255)
                                                                                                                  ggTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCCGGGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgca         <  4:96073/135‑1 (MQ=255)
                                                                                                                  ggTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCCGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgca         <  4:80425/135‑1 (MQ=255)
                                                                                                                  ggTTGACCCACTGCAACGCCGCCGCACCCCAGACGCCCAGGGGTTTGCGGGCACGGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgca         <  6:134190/135‑1 (MQ=255)
                                                                                                                  ggTTGACCCACGGGACCGCCGCCGCACCCCAGAAGCCCAGCGGTTGGCGGGCACGGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgca         <  8:72944/135‑1 (MQ=255)
                                                                                                                  ggTTCACCCACTGCAACGCCGCCGCCCCCCTGACGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgca         <  6:299268/135‑1 (MQ=255)
                                                                                                                    ttcaCCCACTGGACCGCCGCCGCACCCCAGAAGCCCCGCGGTTTGGGGGCACTGGCCGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgcagc       <  2:15139/132‑1 (MQ=255)
                                                                                                                    ttGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCgcagc       <  8:205954/135‑1 (MQ=255)
                                                                                                                         ccACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCGCAGCACCgt  >  3:81562/1‑135 (MQ=255)
                                                                                                                         ccACTGGAACGCCGCCGCACCCCACACGCCCCGCGGTTTGCGGGCACGGGCCGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCGCAGCACCgt  <  2:106624/135‑1 (MQ=255)
                                                                                                                          cACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCGCAGCACCgt  >  2:12442/1‑134 (MQ=255)
                                                                                                                                 |                                                                                                                              
CGCTGGGCAAGTTGACCAAAGCGAACAAGGCCGCAATGACGATCACGTTGGTTACATAGCCTTGCGCTGGCGTGTAGGTGGTAATCGCCCCTACCGCCATCACCCAAGCCTTGGGGTTGACCCACTGGAACGCCGCCGCACCCCAGAAGCCCAGCGGTTTGCGGGCACTGGACGCGGCCGTGCCGACCGGCCCGGAAGTGGCGATCTTCCAGGCCAGGTAAAGCAGATAGGCGGCGCCAGCGTAGCGCAGCACCGT  >  KT2440/3849981‑3850236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: