Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,912,321 T→G 20.4% S820A (TCG→GCG)  mexB_2 → Multidrug resistance protein MexB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,912,3210TG20.4% 127.8 / 4.6 88S820A (TCG→GCG) mexB_2Multidrug resistance protein MexB
Reads supporting (aligned to +/- strand):  ref base T (36/30);  new base G (11/9);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCGAAGACTTGCTGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGT  >  KT2440/3912191‑3912450
                                                                                                                                  |                                                                                                                                 
ggTCGAAGACTTGCTGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGCCCCGCTACAACGGTTACCCGGCGGGGTCGag                                                                                                                               >  3:65003/1‑134 (MQ=255)
ggTCGAAGACTTGCTGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGAt                                                                                                                               >  1:211343/1‑135 (MQ=255)
    gAAGACTTGCTGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCa                                                                                                                           >  7:241130/1‑135 (MQ=255)
     aaGACTTGCTGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAg                                                                                                                          >  3:98463/1‑135 (MQ=255)
     aaGACTTGCTGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAg                                                                                                                          >  6:316770/1‑135 (MQ=255)
     aaGACTTGCTGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGGGCTCTCAg                                                                                                                          >  8:263454/1‑135 (MQ=255)
         cTTGCTGAACATTCACGGGCGCAACGACAGCGGCAAGATGGGGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGa                                                                                                                      <  7:263454/135‑1 (MQ=255)
              tGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACACCGGTTACCCGGCGGTGGGGCTCGCAGGCGCGccgg                                                                                                                 >  7:305410/1‑133 (MQ=255)
              tGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTg                                                                                                                 <  4:44812/135‑1 (MQ=255)
               gAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTgc                                                                                                                >  2:188918/1‑135 (MQ=255)
               gAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTgc                                                                                                                >  2:194381/1‑135 (MQ=255)
               gAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTgc                                                                                                                >  5:112828/1‑135 (MQ=255)
                 aCATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTTGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTgc                                                                                                                <  3:247164/133‑1 (MQ=255)
                 aCATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTgc                                                                                                                <  3:166055/133‑1 (MQ=255)
                     tCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCgg                                                                                                          <  4:300318/135‑1 (MQ=255)
                     tCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGGCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCgg                                                                                                          <  8:38476/135‑1 (MQ=255)
                       aCGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGCTCTCAGGCGAGCCTGCCGCCCGCt                                                                                                        >  3:188969/1‑135 (MQ=255)
                       aCGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGTCGAGCCTGCCGCCGGCt                                                                                                        >  4:99744/1‑135 (MQ=255)
                          tGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACa                                                                                                     <  8:91122/135‑1 (MQ=255)
                             gcAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGCGCTCTCAGGCGAGCCTGCCGCCGGCGACACCt                                                                                                  >  6:207063/1‑135 (MQ=255)
                               aaCGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCACCTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCGGCCGCCGGCGACaccgcg                                                                                                >  7:130514/1‑130 (MQ=255)
                               aaCGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGCTCTCAGGCGCGCCTGCCGCCGGCTACAGCTCg                                                                                                >  3:291334/1‑135 (MQ=255)
                               aaCGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGCTATCCGGCGCGCCAGCCCCCGGCGACCGcgcc                                                                                                >  6:193171/1‑132 (MQ=255)
                                    cAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGa                                                                                           >  8:150901/1‑135 (MQ=255)
                                     aGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAg                                                                                          <  8:61110/135‑1 (MQ=255)
                                     aGCGGCAAGATGGTGCCACTGGGCGCGTGCGTGCAGGCCAGGTGGGGCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAg                                                                                          <  1:127219/135‑1 (MQ=255)
                                      gCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGCGCTCTCAGGCGCGCCTGCCGCCGGCGTCAGCTCGGGCGCgg                                                                                         >  5:286226/1‑135 (MQ=255)
                                          cAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCAGCCGCCGGCTACAGCTCGGGCGAGGCCAt                                                                                     >  3:127578/1‑135 (MQ=255)
                                          cAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGGGGACACAGGCGCGCCTGCCCCCGGCGACCGGACCGGCCAGGccct                                                                                     >  8:287494/1‑133 (MQ=255)
                                            aGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATgg                                                                                   <  2:211343/135‑1 (MQ=255)
                                                    ccccTGGGCGGGTGCGGGCCGGCCTGGTGGGCCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTc                                                                           <  5:259172/132‑1 (MQ=255)
                                                        tGGGCGCGGTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCGGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGc                                                                       <  4:252915/135‑1 (MQ=255)
                                                          ggCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGCTCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCgg                                                                     >  6:120634/1‑135 (MQ=255)
                                                              cgTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCtgg                                                                 <  1:188918/135‑1 (MQ=255)
                                                              cgTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCtgg                                                                 <  1:194381/135‑1 (MQ=255)
                                                               gTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGCGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCCTGGCCGCAGTCGCGCGGCtggt                                                                >  6:133985/1‑135 (MQ=255)
                                                               gTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCtggt                                                                >  7:65417/1‑135 (MQ=255)
                                                               gTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCGACAGCTCGGGCGCGGCCCTGGCCGAAGTCGAGCGGCtggt                                                                >  6:61468/1‑135 (MQ=255)
                                                                ttCGTGCAGGCCAGGTGGGTCAGCGGCCCGTTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCtggtg                                                               <  4:260845/135‑1 (MQ=255)
                                                                  cGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGgc                                                             >  8:172003/1‑135 (MQ=255)
                                                                   gTGCAGGCCAGGTGGGTCAGCGGCCCGGGGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTAGCTCTCAGGCGAGCCAGCCGCCGGCTACAGCTCCGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGgcg                                                            >  3:169027/1‑135 (MQ=255)
                                                                      ccggCCCGGTGGGGCCGCGGCCCGGTGCAACTGACCCGCTACACCGGTTCCCCGGCGGTGTCGATCTCCGGCGAGCCGGCCGCCGGCTACCGCGCGGGCGAGGCCAGGGCCGAAGGCGAGCGGCTGGTGGCGCAg                                                         <  4:291334/133‑1 (MQ=255)
                                                                      cAGGCCCGGGGGGGCCGCGGCCCGGTGCAACGGACCCGCTCCACCGGTTACCCGGCGGGGGCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCGCGGGCGCGGCCATGGCCGAAGGCGAGCGGCTGGTGGCGCAg                                                         <  8:305410/135‑1 (MQ=255)
                                                                      cAGGCCAGGTGGGTCAGCGGCCCGGTGCAAGTGACCCGGTACTACGGTTCCCGGGCGGGGTCGATCTCAGGCGAGCCGGCCGCCGGCTACAGCGCGGGCGAGGCCATGGCGGAAGTCGAGCGGCTGGTGGCGCAg                                                         <  4:77274/135‑1 (MQ=255)
                                                                      cAGGCCAGGTGGGGCCGCGGCCCGGTGCAACTGACCCGCTACACCGCTTACCCGGCGGGGCCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGCCGAGCGGCTGGTGGCGCAg                                                         <  7:242494/135‑1 (MQ=255)
                                                                      cAGGCCAGGTGGGGCCGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAg                                                         <  6:160308/135‑1 (MQ=255)
                                                                       aGGCCAGGGGGGGCCGCGGCCCGGTGCAACTGACCCGCTACAACGGATCCCCGGCGGTGTCGATCTCAGGCGAGCGTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAg                                                         <  8:16291/134‑1 (MQ=255)
                                                                           cAGGGGGGTCACCGGCCCGGGGCAACTGACCCGCTACAACGGTTACCCGGCGGGGGCGATCTCCGGCGAGCCTGCCTCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTAcc                                                    <  8:264401/135‑1 (MQ=255)
                                                                            aGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGc                                                                                                                    >  1:264684/1‑70 (MQ=255)
                                                                            aGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGc                                                                                                                    <  2:264684/70‑1 (MQ=255)
                                                                            aGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTAccg                                                   >  4:208886/1‑135 (MQ=255)
                                                                            aGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGCGATCTCACGCGAGCCGGCCGCCGGCTACCGCTCGGGCGCGGCCCTGGCCGCAGTCGAGCGGGTGGTGGCGCCGCTAccc                                                   >  6:208644/1‑134 (MQ=255)
                                                                              gTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGCGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCCGGGCCGCAGTCGAGCGGCTGGGGGGGCAGCTTccggc                                                 >  1:23939/1‑135 (MQ=255)
                                                                              gTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGCGCGGCTGGTGGCGCCGCTAccggc                                                 >  1:220901/1‑135 (MQ=255)
                                                                                ggtacAGCGGCCCGTTGCAACTCCCCCGCTACACCGGTTACCCGGCGGTGTCTATCGCTGGCGAGCCGCCCGCCGGCTACAGCTCGGGCGAGGCCAAGCCCGCAGTGTCGCGGCTGGGGGCGCAGCTAccggccg                                               <  6:309046/131‑1 (MQ=255)
                                                                                 gggcggCGGCCCGGGGCAACTGACCCGCTACAACGGTTCCCCGGGGGTGGCGAGCTCAGGCGCGCGGGCCGCCGGCTACAGCTCGGGCGTGGCCATGCCGGAAGTCGAGGGGCTGGTGGCGCAGCTAccggccgg                                              <  7:63346/130‑1 (MQ=255)
                                                                                 ggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTGGAGCGCAGGCGCGCCTGCCGCCGGCGACAGCGCGGGCGAGGGCCTGGGCGGAGTCGCGGGGCTGGGGGCGCAGCGAcccggcgg                                              >  6:208017/1‑135 (MQ=255)
                                                                                 ggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGGGCGGCCATGGCCGCAGTCGCGCGGCTGGTGGCGCCGCTAccggccg                                               >  4:172959/1‑134 (MQ=255)
                                                                                 ggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTAccggccgg                                              <  4:188969/135‑1 (MQ=255)
                                                                                 ggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGGGAGCTCCGGCGGGCCTGCCGCCGGCTACCGCGCGGGGCAGGGCCTGGCCGGACGCGCGCGGCGGGTGGCGCCGCTAccggcccg                                              >  6:212099/1‑133 (MQ=255)
                                                                                 ggTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGCGCTCTCAGGCGCGCCGGCCGCCGGCGACCGCTCGGGCGCGGCCAGGGCCGCAGGAGAGCGGCGGGGGGCGCCGCGTccggcccg                                              >  7:300386/1‑133 (MQ=255)
                                                                                   tCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCCGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCTAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTa                                            <  7:115472/135‑1 (MQ=255)
                                                                                     aGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACGCGGCGGTGGCGATCTCCGGCGCGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCAGGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTAcc                                          <  7:323274/135‑1 (MQ=255)
                                                                                      gCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGGTCTCAGGCGCGCCTGCCGCCGGCGACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGGGGCGCAGCTACCGGCCGGTTCCg                                         >  7:170403/1‑135 (MQ=255)
                                                                                      gCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCg                                         >  4:278549/1‑135 (MQ=255)
                                                                                      gCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCg                                         >  4:278554/1‑135 (MQ=255)
                                                                                      gCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGGGCTCTCAGGCGCGCCTGCCGCCGGCGACAGCTCGGGCGCGGCCATGGCCGCAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGGACCg                                         >  6:6141/1‑135 (MQ=255)
                                                                                      gCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGGGGCTCGCAGGCGAGCCGGCCCCCGGCGACACCGCGGGCGCGGCCCGGGCCGACGTCGAGCGGGGGGTGGCGCCGCTAccgcccggggccg                                         >  7:149275/1‑130 (MQ=255)
                                                                                        ggCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGAGGCTGGTGGCGCAGCTACCGGCCGGTACCGGt                                       >  7:57809/1‑135 (MQ=255)
                                                                                        ggCCCGGTGCAACTGACCCGCTACAACGGTCACCCGGCGGGGTCGATCGCAGGCGAGCCGGCCGCCGGCTACAGCGCGGGCGCGGCCATGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGt                                       <  2:308495/135‑1 (MQ=255)
                                                                                            cGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGCTCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGCGCGGCGGGTGGCGCAGCTACCGGCCGGTACCGGTCTg                                    >  6:136532/1‑134 (MQ=255)
                                                                                               tGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTAAGGCGAGCCTGCCGCCGGCGACAGCTCGGGCGAGGCCATGGCCGAAGTGCAGCGGGTGGGGGCGCAGCTACCGGCCGGTACCGGTCTGGCAt                                >  2:98975/1‑135 (MQ=255)
                                                                                                gCAAGTGACCCGATACAAGGGTTCCCCGGCGGTGTCGTAGAGAGGCGAGCCTGCGGCCGGCTACAGCTCGGGCGAGCCCTTGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATg                               <  5:67449/135‑1 (MQ=255)
                                                                                                gCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTGTGGAATg                               >  8:50971/1‑135 (MQ=255)
                                                                                                gCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATg                               <  8:270383/135‑1 (MQ=255)
                                                                                                ccAACGGCCCCGCAACAACGGTCACCCGGCGGTGGCGAGCTCAGGCGAGCCTGCCGCCGGCAACCGCTCGGGCGAGGCCAGGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATg                               <  7:38883/134‑1 (MQ=255)
                                                                                                  aaCTGACCCGCAACAAGGTTTACCCGGGTGTGTCTATGTCAGGAGTGCCTGCCGCCGTTTACAGCTGGGGCGTTGCCATGGCGGATTTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATTGa                             <  4:127578/135‑1 (MQ=255)
                                                                                                    cggACCCGCTACACCGGTCCCCGGGCGGGGGCGATCTCCGGCGCGCGTGCGGCCGGCTACCGCGCGGGCGCGGCCAGGGCCGAGGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGAcc                           <  5:183849/133‑1 (MQ=255)
                                                                                                    cTGACCCGCTACAACGGTAACCCGGCGGGGTCGAGCTCAGGCGCGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCTTGGCCGAAGGCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGAcc                           <  2:293992/135‑1 (MQ=255)
                                                                                                     ttaCCCGCGACAACGGTCACCCGGGGGTGTCTCTCGCTGCCGCGCCGGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCTAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCg                          <  6:22096/133‑1 (MQ=255)
                                                                                                      gACCCGCTACAACGGTTACCCGGGGGTGTCGATCTCCGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGCGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCgg                         <  1:26216/135‑1 (MQ=255)
                                                                                                      gACCCGCTACAACGGTTACCCGGCGGTGGCGATCTAACGCGCGCCTGCCGCCCGCGACCGCTGCGGCGCGGCCATGGCCGAAGTCGCGCGGCGGGTGGCGCAGCTACCCGCCGGGACCCGGCGGGCATGGCCCgg                         >  7:252752/1‑135 (MQ=255)
                                                                                                          cGCTACAACGGTTACCCGGCGGTGTAGATCTAAGGCCAGCCTGCAGCCGGCTACAGCTCGGGCGACGCCATGGCCGAAGTCCAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGAGCGGCCTg                     >  6:316869/1‑135 (MQ=255)
                                                                                                              aCAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCAc                 <  1:34449/135‑1 (MQ=255)
                                                                                                                      tccccGGCGGTGCCGAGCCCCGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCAGGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAg         <  2:136166/133‑1 (MQ=255)
                                                                                                                       ccgcgggggTGGCGAGCTCGGGCGCGCCGGCCGCCGGCTACCGGGCGGGCGAGGCCCGGCCCGATGGCGCGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAg         <  5:193171/131‑1 (MQ=255)
                                                                                                                          cggcggTGTCGCTATCACGCGAGCCTGCCGCCGGCTACCGCGCGGGCGAGGCCCTGGCCGAAGTCGCGCGGCTGGTGGCGCAGCTAACGGCCCGTACCGGTCTGGAATGGCCCGGCCTGTCACTGCAGGAGCGGc     >  4:112429/1‑135 (MQ=255)
                                                                                                                             cggGGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCGCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGt  <  4:15890/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
GGTCGAAGACTTGCTGAACATTCACGTGCGCAACGACAGCGGCAAGATGGTGCCACTGGGCGCGTTCGTGCAGGCCAGGTGGGTCAGCGGCCCGGTGCAACTGACCCGCTACAACGGTTACCCGGCGGTGTCGATCTCAGGCGAGCCTGCCGCCGGCTACAGCTCGGGCGAGGCCATGGCCGAAGTCGAGCGGCTGGTGGCGCAGCTACCGGCCGGTACCGGTCTGGAATGGACCGGCCTGTCACTGCAGGAGCGGCTGT  >  KT2440/3912191‑3912450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: