| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 169,792 | G→A | 8.9% | intergenic (+756/+2931) | PP_RS08750 → / ← fhuA_1 | tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 169,792 | 0 | G | A | 8.9% | 254.7 / 13.7 | 90 | intergenic (+756/+2931) | PP_RS08750/fhuA_1 | tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor |
| Reads supporting (aligned to +/- strand): ref base G (39/43); new base A (6/2); total (45/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01 | |||||||||||
ACTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAGGG > KT2440/169661‑169919 | acgttGTCTGACTATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt < 7:66755/132‑1 (MQ=17)aCTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGt < 7:335810/135‑1 (MQ=32) tttGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTAc < 1:75235/135‑1 (MQ=32) ttGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTa > 2:75235/1‑133 (MQ=32) ttGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTa > 1:278498/1‑133 (MQ=32) gAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGt > 4:204537/1‑135 (MQ=255) atatGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTg < 2:140206/134‑1 (MQ=32) tatGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTggg < 2:185835/135‑1 (MQ=32) ggggggACCATCCTCCAAGGCTAAATACTACTGACTGACAGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAg < 4:256985/134‑1 (MQ=17) cATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGCAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTg > 7:210805/1‑135 (MQ=17) gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc < 4:165767/117‑1 (MQ=32) gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc > 3:165767/1‑117 (MQ=32) gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc > 5:307193/1‑117 (MQ=32) gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc < 6:302172/117‑1 (MQ=32) gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc < 6:307193/117‑1 (MQ=32) gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc > 5:302172/1‑117 (MQ=32) gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg < 2:210359/135‑1 (MQ=32) tAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGc > 5:134104/1‑134 (MQ=32) tAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCg < 6:154298/135‑1 (MQ=32) atcctactGCCTGACCGATAGTGAACCAGTACCGCGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc < 6:280749/132‑1 (MQ=12) aTACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc < 4:207951/135‑1 (MQ=32) tactacTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg > 6:28054/1‑129 (MQ=32) tactacTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg < 5:28054/129‑1 (MQ=32) actcctGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCt > 3:238571/1‑135 (MQ=11) actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTAc > 4:298047/1‑96 (MQ=33) actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTAc < 3:298047/96‑1 (MQ=33) actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc > 2:56054/1‑133 (MQ=32) actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc < 1:56054/133‑1 (MQ=32) actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCt > 8:321142/1‑135 (MQ=32) tactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCtt < 8:210805/134‑1 (MQ=32) tgactgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGt > 8:190482/1‑135 (MQ=11) actgacCGATAGTGAACCAGTACCGTGAGTGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGAGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta > 2:67658/1‑134 (MQ=17) actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAg > 2:191299/1‑101 (MQ=32) actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAg < 1:191299/101‑1 (MQ=32) actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta > 5:72275/1‑134 (MQ=32) actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta > 1:218448/1‑134 (MQ=32) actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta > 7:248535/1‑134 (MQ=32) ctgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta < 6:313036/133‑1 (MQ=32) gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAAt > 5:224474/1‑135 (MQ=32) gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATggg > 4:283804/1‑134 (MQ=11) gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGc > 2:254478/1‑78 (MQ=34) gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGc < 1:254478/78‑1 (MQ=34) aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAACGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGATGACTGCGTACCTTTTGTATAATGGGTc < 7:130028/135‑1 (MQ=17) aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc < 4:159365/135‑1 (MQ=32) gAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGa < 7:48781/135‑1 (MQ=32) cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACtt > 4:31860/1‑134 (MQ=32) cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACt > 3:69643/1‑133 (MQ=32) cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACt < 4:69643/133‑1 (MQ=32) tACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat < 5:34944/135‑1 (MQ=32) ccGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTGAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTc > 2:77657/1‑135 (MQ=11) ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAGCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat > 2:291796/1‑133 (MQ=17) ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTggg < 1:129079/77‑1 (MQ=34) ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTggg > 2:129079/1‑77 (MQ=34) ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat > 8:132899/1‑133 (MQ=32) tGAGGGAAAGGCTAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGt < 3:204537/135‑1 (MQ=11) tGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGt < 7:190482/135‑1 (MQ=11) gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTgg > 8:218060/1‑134 (MQ=32) gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc > 4:312296/1‑135 (MQ=32) gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc < 2:54530/135‑1 (MQ=32) gAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGcaa > 2:191542/1‑133 (MQ=11) aaaGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAg < 7:132899/135‑1 (MQ=32) aaaGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAg < 7:328657/135‑1 (MQ=32) aGGCGATAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTAGAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt < 2:143552/135‑1 (MQ=17) cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt < 7:35371/84‑1 (MQ=33) cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt > 8:35371/1‑84 (MQ=33) cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCtttt > 3:195903/1‑95 (MQ=33) cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCtttt < 4:195903/95‑1 (MQ=33) cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt < 3:145577/120‑1 (MQ=32) cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt > 4:145577/1‑120 (MQ=32) cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGa > 4:16208/1‑135 (MQ=32) aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGTGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa < 1:139020/135‑1 (MQ=17) aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt > 4:103198/1‑110 (MQ=32) aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt < 3:103198/110‑1 (MQ=32) aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGGTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa < 6:158476/135‑1 (MQ=17) aGTGAAATAGAACCTGAAACCGTGGGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa < 1:298720/135‑1 (MQ=17) tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa < 5:270899/135‑1 (MQ=32) tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa < 8:338131/135‑1 (MQ=32) tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa < 3:55082/135‑1 (MQ=32) tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa < 2:218448/135‑1 (MQ=32) gAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAg > 8:102017/1‑135 (MQ=32) aaTAGAACCTGAAACCGTGTGCGAACAAGCAGTGGGAGCCTACTTTGTTTGGTGACTGCGTAGCGTTAGTATAATGCGTCAGCGACTGATAATCAGTGGAGAGATTAACAGAATAGGGGAGGCGTAGCGAAAg > 2:143877/1‑133 (MQ=11) aaCCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTc > 6:247808/1‑134 (MQ=32) ccTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTa < 3:31860/135‑1 (MQ=32) cTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTa > 8:236368/1‑134 (MQ=32) cTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTa > 4:225902/1‑134 (MQ=32) cTGAAACCGTGTGCGTACAAGCACTGGTAGCCTACTTTGTTTGGTGAATGCGTAGCTTATGTATAATCCCTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAATCGAGACTTa > 1:75715/1‑134 (MQ=11) gAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATa > 1:231196/1‑135 (MQ=32) aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc < 7:73870/67‑1 (MQ=35) aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc > 8:73870/1‑67 (MQ=35) aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAggg > 1:86623/1‑135 (MQ=32) | ACTTTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAGGG > KT2440/169661‑169919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |