Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,029,463 A→C 26.5% D290A (GAC→GCC)  mcpU_3 → Methyl‑accepting chemotaxis protein McpU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,029,4630AC26.5% 95.5 / 5.2 75D290A (GAC→GCC) mcpU_3Methyl‑accepting chemotaxis protein McpU
Reads supporting (aligned to +/- strand):  ref base A (26/26);  new base C (11/10);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTGGGGCG  >  KT2440/4029329‑4029588
                                                                                                                                      |                                                                                                                             
gCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATTCCAGCAAGCTGGCCCAGCGCTTCGCCCCGGTAGa                                                                                                                               >  8:183264/1‑135 (MQ=255)
  aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCGCCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGAcc                                                                                                                             >  7:338931/1‑135 (MQ=255)
  aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGAcc                                                                                                                             >  4:221923/1‑135 (MQ=255)
  aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCGTCGACCAGGGAGAcc                                                                                                                             >  3:25832/1‑135 (MQ=255)
     cACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCTTCGCCCAGGTAGCCCCGa                                                                                                                          >  4:327880/1‑135 (MQ=255)
      agggAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGAc                                                                                                                         <  8:120154/132‑1 (MQ=255)
      aCTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCCCGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGAc                                                                                                                         <  8:278838/135‑1 (MQ=255)
         gAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGccc                                                                                                                        >  3:99717/1‑133 (MQ=255)
            ccAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGTCTGGCTACCGCGCCGATGCCCGCAATCTGGGCCACCCCGTCGCCCCCGGAGGCACCACCAAAgg                                                                                                                   >  4:174715/1‑135 (MQ=255)
             cAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGCCCCGACCAAAGgc                                                                                                                  >  4:120964/1‑135 (MQ=255)
              cggaggCCAGCCGCCGCCTCTACCACGGCCGCACCCCGGGCGGCATTCTCCCCCCGGGCGGTGTGTGGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCCGGTAGACCCGACCAAAcgcg                                                                                                                 <  5:10349/134‑1 (MQ=255)
              aggaggCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGgcg                                                                                                                 <  8:32245/135‑1 (MQ=255)
                  ggCCAGCCGCAGCCTCTACGACGGCCGCACCACGGGCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGa                                                                                                             <  7:173275/135‑1 (MQ=255)
                   gCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAg                                                                                                            >  1:95335/1‑135 (MQ=255)
                   gCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGGCTGTTGGCTGGCTACAGCGCCGCTTGCAGCCAGCTGGCCCCGCGCAGCGCCCACGTTGCCCCGCCCAACCGCGccccg                                                                                                            >  5:109262/1‑132 (MQ=255)
                        ccGCAGCCTCTACGACGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGt                                                                                                       <  6:177093/135‑1 (MQ=255)
                        ccGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGCCCCGCGCTTCGCCCCGGTTGCCCCGCCCCAAGGCGCCGGGCGGGt                                                                                                       >  7:50733/1‑135 (MQ=255)
                         cGCAGCCTCTACGAAGGCCGCACCACGGTCGGGATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCCGGCAGCTGGCCCAGGGCTTCGGCCCGGTTGCCCCGACCCAAGGCGCCGAGCGGGTg                                                                                                      >  5:194430/1‑135 (MQ=255)
                         cGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCAGCCAGCTGGCCCAGCGGTTCGGCCAGGGAGACCCCACCCAAGGCGCCGAGCTGGTg                                                                                                      >  1:263647/1‑135 (MQ=255)
                                   aCGAAGGCCGCACCACGGCCGGCATTCTCACCCCGGGCGGTCTGTTGGCCGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCt                                                                                              <  5:112711/133‑1 (MQ=255)
                                     gAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCGGTTGGCTGGCTACAGCGCCGATGCCCGCCAGCTGGCCCCGCGCGTGGACCAGGGTGCCCCGCCCCAAGGCGCCGCGCGGGTGGGCACGCGGGcc                                                                                          >  1:129768/1‑135 (MQ=255)
                                     gAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGGCTGTTGGCTGGCTACAGCGCCGCTGCCAGCCAGCTGGCCCCGCGCGTCGACCCGGTGGACCCGCCCCAAGGCGCCGCGCGGGTGCGCCAGCTGGcc                                                                                          >  1:125562/1‑135 (MQ=255)
                                           cGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCAGCGCGTCGCCCAGGGAGCCCCGACCCAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGCTgg                                                                                     >  7:282726/1‑134 (MQ=255)
                                            gCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGACAGCAAGCTGGCCCAGCGCTTCCACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCCATTGCa                                                                                   >  7:37677/1‑135 (MQ=255)
                                             caccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCAGCGCTTCGACCCGGTAGCCCCGCCCAAAGGCGCCGCGCTGGTGCGCAAGCTGGCCGCTGGCaa                                                                                  >  8:14708/1‑135 (MQ=255)
                                               ccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCAGCAAGCTGGCCCAGCGCGTCGACCCGGTAGACCCGCCCAAACGCGCCCAGCGGGTGCGCAAGCAGGCCGAAGGCAAGc                                                                                >  2:198969/1‑135 (MQ=255)
                                               ccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATTCCAGCAAGCTGGCCCCCCGCTTCGCCCAGGTTGACCCGCCCCAACGCGCCGAGCTGGTGCGCAAGCTGGCCGATgggcagc                                                                                >  6:183230/1‑130 (MQ=255)
                                               ccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATTCCAGCAAGCTGGCCCAGCGCGTCGACCAGGTAGACCCGACCAAAGGCGCCGCGCAGGTGCGCAAGCTGGCCGATGGCaa                                                                                  >  4:199773/1‑133 (MQ=255)
                                                cacGGTCGGCATTCTCACCCCGGGCGGGCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCt                                                                               <  6:107017/135‑1 (MQ=255)
                                                  gggTCGGAATTCTCACCCGGGTCGGGCTGTTGGCGGGCTACAGCGCCGATGCCAGCATGCTGGCCCTGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGa                                                                             <  2:185609/134‑1 (MQ=255)
                                                      cGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGaccac                                                                         >  8:275250/1‑135 (MQ=255)
                                                          aTTCTCCCCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATTCCAACCAGCAGGCCCAGCCCGTCGACCACGTAGACCCCACCCAAGGCCCCGACCTGGTGCGCAAGCTGGCCGCTTGCAAGCAGACCAcccac                                                                     >  3:187021/1‑133 (MQ=255)
                                                           ttCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCTTCGCCCAGGGAGACCCGAACAAAGGCGCCGCGCTGGTGCGCAAGCGGGCCGGAGGCCAGCTGCCCCCCCAcc                                                                    >  8:171523/1‑135 (MQ=255)
                                                                aCCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCATCGCCCAGGTAGACCCGACCAAAGGCGCCGAGCAGGTGCGCAAGCTGGCCGCAGGCAAGCTGACCACCCTCCACGAc                                                               >  4:19124/1‑135 (MQ=255)
                                                                  cccGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCg                                                             <  7:208173/135‑1 (MQ=255)
                                                                  cccGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCg                                                             >  7:160519/1‑135 (MQ=255)
                                                                  cccGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCg                                                             <  8:338931/135‑1 (MQ=255)
                                                                  cccGGTCGGTAGTTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCg                                                             <  8:248267/135‑1 (MQ=255)
                                                                  cccGGGCGGGCTGGTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCg                                                             <  4:188857/135‑1 (MQ=255)
                                                                   ccGGTCGGGCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCgg                                                            <  6:49375/135‑1 (MQ=255)
                                                                       tcggtcTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCGTGGCCCAGGGAGCCCCGCCCAAAGGCGCCGCGCTGGTGCGCCAGCAGGCCGAGGGCCAGCGGCCCCCCCTCCCCGcccccgcgc                                                        >  5:51414/1‑129 (MQ=255)
                                                                         ggtcTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGCCCAGCGCGTCGCCCAGGTAGCCCCGCCCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCCAGCGGACCACCCGCCACGACCGGCCgcgc                                                      >  1:294072/1‑135 (MQ=255)
                                                                         ggtcTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGCCCGGCAgcgc                                                      >  7:132846/1‑135 (MQ=255)
                                                                         ggtcTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGCCCCCCCTCCACGACCGGCAgcgc                                                      >  1:205569/1‑135 (MQ=255)
                                                                          gtcTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCt                                                     >  6:61095/1‑135 (MQ=255)
                                                                            gggtgggcgggccACCGCGCCGATGCCCGCAAGCGGGCCCAGCGCTTCGACCAGGTACACCCCACCAACGGCGCCGAGCGGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTg                                                   <  5:18315/130‑1 (MQ=255)
                                                                                tggctggcTACAGCGCCGATGCCATCACGCTGCCCCAGCGGTTCGACCTGGTAGACCCGACCAAAGGCGCCGAGGTGTTGGGCAAGCTGGCGGATGGCAAGCTGACCTCCCTCCACGACCGGCAGCGCTTGAAgg                                               <  6:142657/135‑1 (MQ=255)
                                                                                     ggctACCGCGCCGTGGCCAGCACGGTGCCCCAGCGCTTCGCCCAGGAAGACCCGACCAAAGGCGCCGAGCTGGTTCGAAAGCGGGCCGATGGCAAGCTGACAACCCTCCACGACCGGCAGCGCTTGAAGGTGCTg                                          <  6:43847/135‑1 (MQ=255)
                                                                                     ggctACCGCGCCGATGCCACCAAGCGGCCCCAGCGCTTCCACATGGTACACCCCACACACGGCGCCGAGCGGGTGCGCAAGCGGGCCGTTGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTg                                          <  8:306032/135‑1 (MQ=255)
                                                                                        tACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGACCCGCCCAAAGGCGCCGCGCTGGTGCGCAAGCAGGCCGATGGCAAGCTGCCCACCCACCCCGCCCGGCAGCGGGTGAAGGTGCTggcg                                       >  6:278546/1‑135 (MQ=255)
                                                                                        tACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGCGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTggcg                                       >  2:216169/1‑135 (MQ=255)
                                                                                          ccgcgcCGAGCCCCGCACGGGGGCCCCGCGCTGCCACCAGGACCCCCCCCCCAACGGCGCCGCGCGGGGGCGCAAGCTGGCCGAGGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTggcggc                                     <  5:67222/133‑1 (MQ=255)
                                                                                                cGATGCCCGCAAGCTGGGCCCGCGGGTCGACCAGGGAGGCCCGACCAAAGGCGCCGCGCTGGTGCGCAAGCGGGCCGATGGCCAGCTGACCCCCCTCCCCCACCCGCCGCGGGTGAAGGTTCTGGCGGCATTCCg                               >  6:27890/1‑135 (MQ=255)
                                                                                                cGATGCCAGCAAGCTGGCCCCGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCCAGCTGACCACCCTCCACGCCCGGCCGCGCTTGCAGGTGCTGGCGGCATTCCg                               >  3:51866/1‑135 (MQ=255)
                                                                                                     ccAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCaa                          >  8:108505/1‑135 (MQ=255)
                                                                                                        gaaaGCTGGCCCAGGGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGTGCTGGGGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATcc                       <  8:37677/133‑1 (MQ=255)
                                                                                                        gCATGCTGGCCCAGCGCTTCGACCAGGTACACCCAACAAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCACCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATcc                       <  4:311227/135‑1 (MQ=255)
                                                                                                          acgcgggCCCAGCGCTTCGCCCAGTTAGCCCCGACCAAAGGCGCCGCGCTGGGGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATcc                       <  2:46180/128‑1 (MQ=255)
                                                                                                            gCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCCAGCTGCCCACCCTCCACGCCCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCggc                   >  3:106071/1‑134 (MQ=255)
                                                                                                             cgggCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  5:117923/133‑1 (MQ=255)
                                                                                                             cgggCCCAGCGCTACCACCCGGTACCCCCGACAAACGGCGCCGCGCTGGGGCGCAAGCTGGCCGATGGAAAGCTGACCCCCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  5:183230/133‑1 (MQ=255)
                                                                                                             cggcccccGCGCTTCGACCAGGTACCCCCGACAAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCCCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  3:174956/131‑1 (MQ=255)
                                                                                                             cggccccAGCGCTTCGCCCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  7:201995/131‑1 (MQ=255)
                                                                                                             cTGGCCCCGCGCTTCCACCCGGTAGCCCCGACCAACGGCGCCGAGCGGGGGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  1:129462/135‑1 (MQ=255)
                                                                                                             cTGGCCCAGCGCTTCGACCCGGTCCCCCCGACCAACGGCGCCGAGCTGGGGCGCAAGCTGGCCGATGGAAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  4:323254/135‑1 (MQ=255)
                                                                                                             cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  4:275326/135‑1 (MQ=255)
                                                                                                             cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  3:221923/135‑1 (MQ=255)
                                                                                                              tgccccGGCGCTCCGCCCAGGTCTCCCCGCCCAACGGCGCGGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  3:19124/131‑1 (MQ=255)
                                                                                                              tGGCCCTGCGCTTCGACCAGGAATCCCCGACCAACGGCGCCGTGGTGGTGCGCATGCTGGCCTATGCCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc                  <  1:198969/134‑1 (MQ=255)
                                                                                                               ggCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGc                <  2:95335/135‑1 (MQ=255)
                                                                                                                       gcTTCGACCTGGTCGCCCCTCCCAAAGGCGCCGAGCTGGTGCGCATGCTGGCCGATGGCAAGCTTACCCCCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCt        <  2:272897/135‑1 (MQ=255)
                                                                                                                         ttCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTgg      >  4:92280/1‑135 (MQ=255)
                                                                                                                          tCGACCAGGTCGACCCGACCAACGGCGCCGAGCGGGTGCGCAAGCGGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTggg     <  3:115179/135‑1 (MQ=255)
                                                                                                                            gccccggTAGCCCCGACCAACGGCGCCGCGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTggg     <  7:91574/128‑1 (MQ=255)
                                                                                                                             aCCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTGGGGCg  >  7:334175/1‑135 (MQ=255)
                                                                                                                             aCCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCAGGGGCg  >  7:276659/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                             
GCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTGGGGCG  >  KT2440/4029329‑4029588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: