Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,030,329 T→G 22.9% F579V (TTT→GTT)  mcpU_3 → Methyl‑accepting chemotaxis protein McpU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,030,3290TG22.9% 94.5 / 4.3 61F579V (TTT→GTT) mcpU_3Methyl‑accepting chemotaxis protein McpU
Reads supporting (aligned to +/- strand):  ref base T (12/33);  new base G (13/3);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 2.34e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGGCCTGGCGCAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATGGACCACAGCCACC  >  KT2440/4030197‑4030461
                                                                                                                                    |                                                                                                                                    
gAAGGCCTGGCGCAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCttt                                                                                                                                    >  1:9459/1‑135 (MQ=255)
gAAGGCCTGGCGCAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGAGCCGTGCGGGCGCCCAACGGCCCACCATGATGCCGGGCACCGATGCCATCGACCCTCCCCGCGCCGGGCCGCCCGGCCGTGGCttt                                                                                                                                    >  2:64238/1‑135 (MQ=255)
  aGGCCTGGCGCAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGc                                                                                                                                  <  2:208198/135‑1 (MQ=255)
     ccTGGCGCAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGATCGATCGCCGAACAGACCAACCTGCTGGCGCTCAAAGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGt                                                                                                                               <  7:339432/135‑1 (MQ=255)
     ccTGGCGCAGAACAGCGAGAACATCGGCGCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGt                                                                                                                               <  1:128768/135‑1 (MQ=255)
       tGGCGCAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTgg                                                                                                                             <  2:320372/135‑1 (MQ=255)
          cgcAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCg                                                                                                                          <  1:18762/135‑1 (MQ=255)
           gcAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGc                                                                                                                         >  1:238460/1‑135 (MQ=255)
                  aGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGGGTTGGCGGGGGCGCCGATgcg                                                                                                                  >  3:32675/1‑133 (MQ=25)
                  aGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGCTGAg                                                                                                                  >  4:285307/1‑135 (MQ=255)
                  aGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAg                                                                                                                  <  5:204888/135‑1 (MQ=255)
                    cGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGt                                                                                                                <  2:238460/135‑1 (MQ=255)
                    cGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGt                                                                                                                <  3:221472/135‑1 (MQ=255)
                       gaagaTCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATTGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGACGTGGTCGCCGATGAGGTtcg                                                                                                             >  2:241600/1‑135 (MQ=255)
                       gaagaTCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGGGTTGGCCTGGTCGCCCATGACGTgcg                                                                                                             >  2:240960/1‑135 (MQ=25)
                       gaagaTCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCGTTGGCGTGGTCGCCGATGAGGTggg                                                                                                             >  5:133077/1‑133 (MQ=255)
                       gaagaTCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCGTTGGCCTGGTAGCCCAAGAGGTgcg                                                                                                             >  5:118308/1‑135 (MQ=25)
                            tCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGGGTTGGCGTGGTAGCCGATGAGGTgcggca                                                                                                          >  8:153500/1‑130 (MQ=25)
                            tCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAAcc                                                                                                        >  8:243888/1‑135 (MQ=255)
                              ggCTCGGTACGGGAAGTGAGCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTg                                                                                                      <  5:177756/135‑1 (MQ=255)
                                  cGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGccc                                                                                                  <  8:96739/135‑1 (MQ=255)
                                      aCTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGCGGCCGGCCGTGGGTTTGCCGGGGGGGCCGCAGAGGTTGGCCACCCGGccccccg                                                                                              >  2:125389/1‑131 (MQ=21)
                                       cTGGAAGTGAGCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCGGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGGCGCCGATGAGGTGCGCAACCTGGCCCAgcgc                                                                                             <  5:31088/135‑1 (MQ=255)
                                        tGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTTGCGGGGGCGCCGATTAGGTGCGCAACCAGGCCCCgcgcc                                                                                            >  1:213265/1‑134 (MQ=21)
                                        tGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCGTTGCCGTGGTCGCCGCTGAGGGGCGCCACCTGGCCCCgcgc                                                                                             >  2:210541/1‑134 (MQ=25)
                                            aGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGGCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCa                                                                                        <  2:9459/135‑1 (MQ=255)
                                                 gccGCTCGATCGCCGAACAGACCAACGTGCTGGCGCTCAACGCGGCCATCGAAGCTGCCCGCGCCGTAGAGGCAGGCCGTGGGTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGt                                                                                   <  8:187270/134‑1 (MQ=21)
                                                  ccGCTCGATCGCCGAACAGCCCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGCGAGGCCGGCCGTGGGGTTGCCGTGGTGGCCCAGGCGGTGCGCACCCCGGCCCAGCGCCCGCCGGAGTc                                                                                  >  3:282604/1‑135 (MQ=18)
                                                  ccGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGGTTTGCCGTGGGGGGCGCTGAGGTGCGCCACCAGGCCCCGCGCCCGCAGGGGtg                                                                                  >  1:92591/1‑134 (MQ=21)
                                                  ccGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGGGTTGGCGTGGTGGCCGATGTGGTGGGCCACCGGGGCCAGCGCACTCAGGAGtg                                                                                  >  3:277793/1‑134 (MQ=25)
                                                  ccGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGGGTTGCCGGGGTCGCCGCTGAGGGGCGCCACCGGGCCCAGCGCCCGCACGAGTc                                                                                  >  5:149569/1‑135 (MQ=21)
                                                  ccGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTc                                                                                  >  5:120085/1‑135 (MQ=255)
                                                  ccGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGGCGCCGATGTGGTGCGCCACCTGGCCCCGCGCACTCAGGAGTc                                                                                  >  8:337521/1‑135 (MQ=255)
                                                  ccGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGGCCCCCATGAGTTGCTCACCCAGGGCCCCCGCCCCGAGGAGTc                                                                                  >  2:144192/1‑135 (MQ=18)
                                                  ccGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGGGGTCGCCGCTGAGGTGCGCCACCTGGCCCAGCGCCCTCAGGAGTc                                                                                  >  5:135552/1‑135 (MQ=255)
                                                  ccGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCGTTGCCGTGGTCGCCGATGAGGTGCGCCACCTGGCCCCGCGCCCTCAGGAggc                                                                                  >  8:294983/1‑133 (MQ=21)
                                                     cTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGt                                                                               <  4:14206/135‑1 (MQ=255)
                                                     cTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGt                                                                               <  5:122147/135‑1 (MQ=255)
                                                         aTCGCCGAACAGACCATCCTGCTGGCGCTCAACGCGGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCTATGAGGTGCGCAACCTGGCCCAGCTCACGCAGGAGTCGGTGga                                                                            <  2:179354/134‑1 (MQ=18)
                                                                aaCAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCGTTTGCGGGGTCGCCCCTGTGGTGCGCCACCTGGCCCACCGCCCACAGGCGTGGGGGGGAGAAAccc                                                                    >  5:136396/1‑135 (MQ=21)
                                                                  cAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGt                                                                  <  3:214705/135‑1 (MQ=255)
                                                                         aCCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTTGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCa                                                           <  3:230045/135‑1 (MQ=255)
                                                                            tgctgGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCg                                                        <  5:314627/135‑1 (MQ=255)
                                                                               tgGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGaa                                                      <  7:243888/134‑1 (MQ=255)
                                                                               tgGCGCGCAACGCTGCCATCGATGCTGCCCGCGCCGGAGAGTCCGGCCGTGGCTTTGCGGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGaa                                                      <  5:338534/134‑1 (MQ=255)
                                                                                         cGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGaa                                           <  6:120085/135‑1 (MQ=255)
                                                                                         cGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCGTTGGCGTGGTGGCGGATGAGGTGGGCCACCTGGGCCCGCGCACTCAGGGGTCGGTGGGAGCAACCCCGCACGTGAGCGAAGCCCGGccgca                                           >  5:65475/1‑131 (MQ=255)
                                                                                                 tCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCCCCCTGGCCCCGCGCCCGCAGGAGTGGGTGGCAGAAACCCCACACGTCAACGAAGCCCGGCAGACCCGCAccc                                   >  4:230815/1‑135 (MQ=18)
                                                                                                        ggCCCGCGCCGGAGAGGCCGGCCGGGGCTTGGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGGCGGTGGAAGAAACCCGTCAGGTCAGCGAAGCCCTGCAGAACGGCACCCGCGAAGt                            <  6:36257/134‑1 (MQ=255)
                                                                                                          cccGCGCCGGAGCGGCCGGCCGTGGCGTTGCCGTGGGCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTgg                          <  1:155693/135‑1 (MQ=255)
                                                                                                          cccGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTgg                          <  2:150214/135‑1 (MQ=255)
                                                                                                              cgcCGGAGCGGCCGGCCGTGGCGGTGCCGGGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCgg                      <  7:337521/135‑1 (MQ=255)
                                                                                                               gcCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGgc                     <  4:38356/135‑1 (MQ=255)
                                                                                                                ccGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGgcg                    <  6:118308/135‑1 (MQ=255)
                                                                                                                     gagGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATg               <  7:210541/135‑1 (MQ=255)
                                                                                                                           ggccgTGGCGTTGCCGTGGTAGCCGATGAGGTGCGCAACCGGGCCCAGCGCCCGCAGGAGTCGGTGGAAGAAACCCCTGAGGTCATCGACGCCCTGCAGAACGGCCCCCGCGAAGTGGGCGGCGCCATGGACcac         >  8:37946/1‑135 (MQ=37)
                                                                                                                             ccgTGGCGTGGCCGTGGGCGCCGATGAGGGGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGACACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATGGACCACAg       <  1:230974/135‑1 (MQ=255)
                                                                                                                                 ggCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATGGACCACAGCCAc   <  1:105772/135‑1 (MQ=255)
                                                                                                                                 ggCTTTGCCGGGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATTGACCACAGCCAc   <  3:104051/135‑1 (MQ=255)
                                                                                                                                  gCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATGGACCACAGCCAcc  <  1:64238/135‑1 (MQ=255)
                                                                                                                                  gCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATGGACCACAGCCAcc  <  1:103888/135‑1 (MQ=255)
                                                                                                                                  gCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATGGACCACAGCCAcc  <  1:125389/135‑1 (MQ=255)
                                                                                                                                  gCTTTGCCGGGGTAGCCGATGAGGTGCGCAACCTGTCCCAGCGCAATCAGGAGTCGGTGGAAGAAACCCGGGAGGTCATCGACGCCCTGCAGAACGGCACCCGCTAAGTGGGCGGCGCCATGGACCACAGCCAcc  <  8:144425/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GAAGGCCTGGCGCAGAACAGCGAGAAGATCGGCTCGGTACTGGAAGTGATCCGCTCGATCGCCGAACAGACCAACCTGCTGGCGCTCAACGCTGCCATCGAAGCTGCCCGCGCCGGAGAGGCCGGCCGTGGCTTTGCCGTGGTCGCCGATGAGGTGCGCAACCTGGCCCAGCGCACTCAGGAGTCGGTGGAAGAAACCCGTCAGGTCATCGAAGCCCTGCAGAACGGCACCCGCGAAGTGGTCGGCGCCATGGACCACAGCCACC  >  KT2440/4030197‑4030461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: