Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,079,259 G→A 5.9% A105T (GCC→ACC)  dadA → D‑amino acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,079,2590GA 5.9% 210.7 / 2.1 73A105T (GCC→ACC) dadAD‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (28/38);  new base A (2/2);  total (33/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GGCAACTGAGCGCACTGCTGCGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTC  >  KT2440/4079125‑4079390
                                                                                                                                      |                                                                                                                                   
ggCAACTGAGCGCACTGCTGCGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGgcg                                                                                                                                     <  3:304403/135‑1 (MQ=255)
ggCAACTGAGCGCACTGCTGCGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGGGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGgcg                                                                                                                                     <  2:286200/135‑1 (MQ=255)
 gCAACTGAGCGCACTGCTGCGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGgcgc                                                                                                                                    <  7:210141/135‑1 (MQ=255)
  cAACTGAGCGCACTGCTGCGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCCCCCGGGCGCCTGGCCGGcgcc                                                                                                                                   >  5:283802/1‑135 (MQ=255)
  cAACTGAGCGCACTGCTGCGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGcgcc                                                                                                                                   >  1:94750/1‑135 (MQ=255)
         gcgcACTGCTGCGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGGCGGCACCCGGGCGCCTGGCCGGCgccgccgcc                                                                                                                             <  8:298318/134‑1 (MQ=255)
                  tgcGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCACGGCCGCACCGTGGCGGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGc                                                                                                                   <  6:257204/135‑1 (MQ=255)
                  tgcGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGc                                                                                                                   <  4:117841/135‑1 (MQ=255)
                  tgcGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGc                                                                                                                   <  7:105800/135‑1 (MQ=255)
                  ggcgcTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGc                                                                                                                   <  7:1392/134‑1 (MQ=255)
                       tACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGACCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGCTATGTTGCCGCTg                                                                                                              >  5:55452/1‑135 (MQ=255)
                       tACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCGGCCGAAGCCGGCACCGTGGCTGGGGCGTTACTGGCGCCAGTGGGCACCCGGGCGCCTGGCGGGCGCCGCCGCCGATATGTTGCCGCTg                                                                                                              <  3:78203/135‑1 (MQ=255)
                            ccTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGGTATGTTGCCGCGGGTGCa                                                                                                         >  1:227562/1‑135 (MQ=255)
                            ccTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGCGATGTTGCCGCTGGTGCa                                                                                                         >  7:299510/1‑135 (MQ=255)
                            ccTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCa                                                                                                         >  8:20962/1‑135 (MQ=255)
                            ccTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCGGGCCGGCCCCCCCGCCGAGAAGTTTGCCCTGGTggc                                                                                                         >  5:23584/1‑133 (MQ=255)
                              ttacccgcccgcCCGAGGGGCGCTACCGCCGGGCGCACGGGCCCAAGGCCGCACCGTGGCGGGGGCGTTACTGGCGCCAGGCGGCACCCGGGCGCCGGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAgc                                                                                                       <  6:185965/131‑1 (MQ=255)
                              tGACCCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAgc                                                                                                       <  3:267559/135‑1 (MQ=255)
                              tGAACCGCCAGCCCGTGGGGCGCTACAGCCTGGCGCACCTGCCCACGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGGCGGCACCCGGGCGCGTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAgc                                                                                                       <  4:192099/135‑1 (MQ=255)
                              tGAACCGCCAGCCCGATGTGCGCTACCGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCCCCCGGGCGCCTGGCCCGCGCCGCCCCCGCTATGTTGCCGCTGGTGCCgc                                                                                                       >  7:182481/1‑135 (MQ=255)
                              tGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGGCGCCGCCGCTTTGTTGCCGCTGGTGCAgc                                                                                                       >  2:105530/1‑135 (MQ=255)
                              tGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGCTATGTTGCCGCTGGTGCAgc                                                                                                       >  2:100909/1‑135 (MQ=255)
                              tGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAgc                                                                                                       >  7:111990/1‑135 (MQ=255)
                                     ccAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTgtgt                                                                                                <  8:237186/135‑1 (MQ=255)
                                        gCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCCCCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCGGGCCGGCGCCGCCGCCGATATGTTTGCGCTGGTGCAGCGCGGTGGcga                                                                                             >  8:9258/1‑135 (MQ=255)
                                        gCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGatat                                                                                                                        >  4:189194/1‑108 (MQ=255)
                                        gCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGatat                                                                                                                        <  3:189194/108‑1 (MQ=255)
                                        gCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTcga                                                                                             >  4:299168/1‑135 (MQ=255)
                                        gCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCGGTGTcga                                                                                             >  6:60174/1‑135 (MQ=255)
                                                  cgcTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTCCTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGAc                                                                                   <  5:75730/135‑1 (MQ=255)
                                                        aGCCTGGCGCACCTGACCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCt                                                                              <  6:55452/134‑1 (MQ=255)
                                                          ccTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCCCCCGGGCGCCTGGGCGGCGCCGCCGCCGATATGTTTCCGCTGGGGCAGCGCGGTGTCGGCGCGCAGGACgcgcggct                                                                           >  1:41720/1‑131 (MQ=255)
                                                                gcACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTgc                                                                     <  7:334042/135‑1 (MQ=255)
                                                                  aCCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCGGGGCGGCGCCGCCGCCGCGATGTTTCCGCGGGGGGAGCGCGGGGTGGCCGCGGCTGACGCGCGGGTCGcgggcg                                                                   >  1:100448/1‑130 (MQ=255)
                                                                    cTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTgccgcc                                                                 <  3:8043/135‑1 (MQ=255)
                                                                      gCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCgg                                                               >  6:316867/1‑135 (MQ=255)
                                                                       cccAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGCTATGTTGCCGCTGGTGGAGCGCTGTGGGGGCGCGCCTGGCGCGCTGATCGCTGCCGCCGGc                                                              >  3:233865/1‑135 (MQ=255)
                                                                        ccAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGcc                                                             <  1:10568/135‑1 (MQ=255)
                                                                        ccAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGcc                                                             <  5:91012/135‑1 (MQ=255)
                                                                              ccGCCCCGGGGCGGGGGCGTTACGGGCGCCCGTCGGCACCGGGGCGCCGGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAg                                                       <  5:34591/135‑1 (MQ=255)
                                                                              ccGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAg                                                       <  8:196868/135‑1 (MQ=255)
                                                                              ccGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAg                                                       <  4:240370/135‑1 (MQ=255)
                                                                              ccGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCGGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAg                                                       <  3:30892/135‑1 (MQ=255)
                                                                              ccGCACCGTGGCTGTGGCGTTACTGGCGCCAGGCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAg                                                       <  5:109419/135‑1 (MQ=255)
                                                                              ccGCACCGGGGCGGGGGCGGTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCGGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAg                                                       <  6:19392/135‑1 (MQ=255)
                                                                               cGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGCCGCGCATGACGCGCTGATCGCTGCCGCCGGCCGGGAAgg                                                      >  7:333155/1‑135 (MQ=255)
                                                                                    cggggCTGTGGCGTTACTGGCGCCAGTCGGCACCGGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTgg                                                 <  4:286450/132‑1 (MQ=255)
                                                                                    cggggCGGTGGCGTTACTGGCGCCCGGCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTgg                                                 <  6:183961/132‑1 (MQ=255)
                                                                                    cGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGCTATGTTGCCGCTGGTGCAGCGCTGTGTAGCCGAGCATGACGCGCGGATCGCTGCCGCCGGCCGGGAAGGCCTgg                                                 >  6:91012/1‑135 (MQ=255)
                                                                                    cGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCGGGAAGGCCTgg                                                 >  7:184205/1‑135 (MQ=255)
                                                                                    cGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCGGGCCGGCGCCGCCGCCCCTATGTTTCCGCTGGTGCAGCGCGGGGTCGCCGAGCCTGACGCGCTGCTCGCTGCCGCCCGCCGGGGAGGCCggg                                                 >  6:235604/1‑135 (MQ=255)
                                                                                         cTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCCGCGCGGGGTCGCCGCGCCTGACGCGCTGATCGCTGCCGCCGGCCGGGATGGCCTGGGGCaa                                            >  7:115536/1‑135 (MQ=255)
                                                                                         cTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCaa                                            <  3:52369/135‑1 (MQ=255)
                                                                                         cTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCCCCGCCGCCGATATGTTGCCGCGGGTGCAGCGCTGGGTAGACGCGCAAGACCCCCAGATCGCTGCCGCCGGCCGGGAAGGCCTGGTGCaa                                            >  7:326385/1‑135 (MQ=255)
                                                                                          tgtgGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAg                                           <  2:94750/135‑1 (MQ=255)
                                                                                              gCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCaa                                       <  8:288173/135‑1 (MQ=255)
                                                                                               cGTTACTGGCGCCAGTCGGCCCCCGGGCGCCGGGCCGGCGGCGCCGCCGCGATGTTGCCCCGGGGGCAGCGCGGTGTCGCCGCGCAGGCCGCGCTGCTAGCGGCCGCCCGCCGGGCCTGCCGGGTGCAAggccaa                                      >  6:34794/1‑130 (MQ=255)
                                                                                               cGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGCTATGTTGCCGCTGGTGCAGCGCGGTGTCGACGCCCATGACGCGCTGATCGCTGCCGCCCGCCTGGCAGGCCGGGTGCAAGCCaaa                                      >  4:15403/1‑135 (MQ=255)
                                                                                               cGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCaaa                                      >  1:7874/1‑135 (MQ=255)
                                                                                               cGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCCCCCCTATTTTTCCGCTTGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGCTAGCTGCCGCCCGCCGGGTAGGCCAGGGGCAAGCCaaa                                      >  5:298334/1‑135 (MQ=255)
                                                                                                gTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAg                                     <  2:100448/135‑1 (MQ=255)
                                                                                                 ttACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCGGTGTCGCCGAGCATGACGCGCTGATCGCTGCCGCCGGCCGGGAAGGCCTGGTGCAAGCCAAAgg                                    >  3:130376/1‑135 (MQ=255)
                                                                                                    cTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCt                                  <  2:7874/134‑1 (MQ=255)
                                                                                                      ggCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGTCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAg                                     >  7:112595/1‑129 (MQ=255)
                                                                                                      ggCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGTCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAg                                     <  8:112595/129‑1 (MQ=255)
                                                                                                      ggCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGa                               <  8:29595/135‑1 (MQ=255)
                                                                                                            aGTCGGCACCCGGGCGCCTGGCCCGCCCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGCCCAGCCAGACGCGCGGATCGCTGCCGCCGGCCGGGCAGGCCTGGTGCAAGCCAAAGGCTGGATCgcac                         >  2:184854/1‑132 (MQ=255)
                                                                                                                ggCACCCGGGCGCGTGGCGGGCGCCGCCGCCGTTATGTGGCCGATGGTGCAGCGGTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGtt                     <  6:69518/135‑1 (MQ=255)
                                                                                                                ggCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGtt                     <  7:144045/135‑1 (MQ=255)
                                                                                                                ggCACCCGGGCGCCGGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGGGTCGACGAGCATGACGCGCTGATCGCGGCCGCCGGCCTGGACGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGtt                     <  4:103286/135‑1 (MQ=255)
                                                                                                                   aCCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCCCTGTGTCGACGAGCAGGCCGCGCGGCTCGCTGCCGCCGGCCGGGGAGGCCGGGGGCCACGCAAATGCGGGAGCGGAGTCTTCcg                  >  3:302399/1‑135 (MQ=255)
                                                                                                                          cgcCTGGCCGGCACCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGAcccc           >  8:255603/1‑135 (MQ=255)
                                                                                                                          cgcCGGGCCGGCGCGGCCGCCGATATGTTGCCGCTGGTGCAGCGCGGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGAcccc           <  1:74763/135‑1 (MQ=255)
                                                                                                                          cgcCAGGCCGGCACCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGAcccc           >  8:251206/1‑135 (MQ=255)
                                                                                                                                   ggCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTc  <  7:20962/135‑1 (MQ=255)
                                                                                                                                   ggCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTc  <  3:15403/135‑1 (MQ=255)
                                                                                                                                   ggCGCCGCCGCCGATATGTTGCCGCGGGTGCAGCGCGGTGTCGACGAGCATGACGCGCGGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAACGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTc  <  5:253750/135‑1 (MQ=255)
                                                                                                                                   ggCACCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTc  <  7:255603/135‑1 (MQ=255)
                                                                                                                                   ggCACCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTc  <  7:251206/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
GGCAACTGAGCGCACTGCTGCGCTACGGCCTGAACCGCCAGCCCGATGTGCGCTACAGCCTGGCGCACCTGCCCAAGGCCGCACCGTGGCTGTGGCGTTACTGGCGCCAGTCGGCACCCGGGCGCCTGGCCGGCGCCGCCGCCGATATGTTGCCGCTGGTGCAGCGCTGTGTCGACGAGCATGACGCGCTGATCGCTGCCGCCGGCCTGGAAGGCCTGGTGCAAGCCAAAGGCTGGATCGAAGTGTTCCGCGACCCCGCGCTGTTC  >  KT2440/4079125‑4079390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: