Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,092,059	A→C	19.4%	G92G (GGT→GGG)	CJ019_03853 ←	2‑haloacrylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,092,059	0	A	C	19.4%	128.9 / 4.9	77	G92G (GGT→GGG)	CJ019_03853	2‑haloacrylate reductase
Reads supporting (aligned to +/- strand):  ref base A (31/28);  new base C (9/9);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCC  >  KT2440/4091925‑4092186                                                                                                                                       |                                                                                                                                aCGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGCACAGCCGGCCACTGGCATAGGCa                                                                                                                                 >  6:85186/1‑135 (MQ=255)   ggCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCacc                                                                                                                               <  1:193900/135‑1 (MQ=255)   ggCATAGGTGGGTTTCAACTCATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCacc                                                                                                                               <  6:204986/135‑1 (MQ=255)     cATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGACCAGCCGGCCCCTGGCATAGGCcccgc                                                                                                                             >  5:212363/1‑134 (MQ=255)      aTAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCaccgac                                                                                                                            <  8:237134/135‑1 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGc                                                                                                                        >  8:164052/1‑135 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGc                                                                                                                        >  1:200022/1‑135 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGc                                                                                                                        >  3:333169/1‑135 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCgg                                                                                                                         <  3:183723/134‑1 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGc                                                                                                                        <  8:112659/135‑1 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGc                                                                                                                        >  2:240098/1‑135 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGc                                                                                                                        >  6:80251/1‑135 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCCGc                                                                                                                        >  2:117373/1‑135 (MQ=255)          gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTACAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGACCGGc                                                                                                                        >  2:102833/1‑135 (MQ=255)             gtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCAGGAACAGCCGGCCACTGGCATAGGCACCGACCGGcccc                                                                                                                     >  6:305066/1‑135 (MQ=255)             gtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCGCGTCCTCCAACAGCCGCGGCAGCGGCACCCGCCGCTCGGCCGGGCTCACCCGGCCACAGGCCTAGGCCACgccccgcccc                                                                                                                     >  4:191689/1‑135 (MQ=255)                  cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTTTGCCCGGAACCGCCGGCCACAGGCATAGGCCCCCACCCGCCCCAAcgc                                                                                                                >  4:332987/1‑135 (MQ=255)                  cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGCCCCATcgc                                                                                                                >  4:301967/1‑135 (MQ=255)                  cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACGGGCATAGGCACCGACCGGcccccgcgc                                                                                                                >  5:280716/1‑130 (MQ=255)                  cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCATCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATcgc                                                                                                                >  8:11228/1‑135 (MQ=255)                           ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGACCAGCCGGCCACTGGCATAGGCACCGAACGGCCCCCTCGCCTAACCGAc                                                                                                       >  1:67461/1‑135 (MQ=255)                           ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCaccga                                                                                                                             <  4:218941/113‑1 (MQ=255)                           ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCaccga                                                                                                                             >  3:218941/1‑113 (MQ=255)                           ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGCCCCATCGCGTAAGCGAc                                                                                                       >  5:246320/1‑135 (MQ=255)                               gcCGTGATTCCCTTGAACCGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCgg                                                                                                   <  1:67300/135‑1 (MQ=255)                               gcCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGACCCCGCCGCTCGGCCGGGCACCGCCCGCCACAGGCAAAGGCACCGCCCCGCCCCCTCGCGTAACCGcccccg                                                                                                   >  2:164490/1‑133 (MQ=255)                               gcCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCgg                                                                                                   <  7:16781/135‑1 (MQ=255)                                ccGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGCACCGCCGGCCACTGGCATAGGCCCCGCCCGGCCCCCTCGCGTAAGCGCCCCGGt                                                                                                  >  3:194802/1‑135 (MQ=255)                                 cggcaTTCCCTTGAACAGCCCCGCGGCGGCGGCCTCGGCCTCCAACTCCGGCGGCAGCGCACCCAGCCGCGCGGCCGGCAACAGCCGGCCACTGGCATTGGCACCGACCGGCCCCATCGCGTACGCGACCCGGTc                                                                                                 <  8:91579/131‑1 (MQ=255)                                 cGTGATTCCCTTGACCAGCCCCGCGGCGGCGGCCGCGACCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGTACCCGCGGGCCACTGGCATTGCCACCGACCGGCCCCAGCGCGTAAGCGACCCGGTc                                                                                                 <  2:268288/135‑1 (MQ=255)                                 cGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTc                                                                                                 <  1:240098/135‑1 (MQ=255)                                   tGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCCGCGGCAGCGGAACCAGCCGCTCGGCCGGGACCAGCCCGCCACGGGCATATGCACCGCCACGCCCCATCGCGTAACCGACCCGCTCGc                                                                                               >  3:203041/1‑135 (MQ=255)                                      ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGGACAGCCGGCCCCTGGCATAGGCCCCGCCCGGCCCCAACGCGTAAGCGACCCCGGCGcccc                                                                                            >  3:34692/1‑134 (MQ=255)                                      ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACCGCCGGCCACTGGCATAGGCCCCGACCGGCCCCAACCCGTAAGCGCCCCCGTCGCCCa                                                                                            >  2:119724/1‑135 (MQ=255)                                      ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCa                                                                                            >  4:137919/1‑135 (MQ=255)                                      ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCa                                                                                            >  5:313331/1‑135 (MQ=255)                                      ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCCGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCCTCGCGTAAGCGCCCCCGTCGcccc                                                                                            >  1:265903/1‑134 (MQ=255)                                      ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCACCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCa                                                                                            >  4:111287/1‑135 (MQ=255)                                         ccTTGACCAGCACCGCTGCGGCGGCCTGGTCCTCCAACTGCGGCGGCAGCGGAACCCGCCGCTCGGCCGGGAACAGCGGGCCACGGGCATAGGCACCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCt                                                                                         <  6:278006/135‑1 (MQ=255)                                         ccTTCACCAGCACCGCGGGGGCGGCCTCGCCCACCAACTGCGGCGCCAGCGGAACCCGCCGCTCGGCCGGGAACAGCCGGCCACTGCCATAGGCACCGCCCGGCCCCATCGCGAACGCGACCCGGTCGCCCACCt                                                                                         <  6:300863/135‑1 (MQ=255)                                          cTTGAACAGCCCCGCGGGGGCGGCCTCTTCCACAACCTGGGGGGCGAGGGGAACCAGCCGCTCGGCGGGTAACAGCGGGCCAGTGGAATAGGCACAGACGGGCCCCATGGCGTAAGCGACCCGGTCGCCCACCTg                                                                                        <  2:310699/135‑1 (MQ=255)                                                caccACCGCGGGGGCGGCCTCGTCCGCCAACGGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGCAACAGCGGGCCACTGGCATAGCCCCCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGtt                                                                                  <  5:280633/132‑1 (MQ=255)                                                   cACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTtgtg                                                                               <  4:170884/135‑1 (MQ=255)                                                      cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGCCCGGCCCCAACGCGTAAGCGCCCCGGTCGCCCCCCAGCCCCTTGGGCAc                                                                            >  6:39039/1‑135 (MQ=255)                                                      cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGACCGGCCCCCTCGCGTAAGCGACCCGGTCGCCCCCCAGCCCGGTGGGccc                                                                            >  2:319846/1‑133 (MQ=255)                                                      cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACCTTGTGCAc                                                                            >  7:217787/1‑135 (MQ=255)                                                      cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGACCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCAc                                                                            >  1:148314/1‑135 (MQ=255)                                                      cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCCCTCGGCCGGGAACAGCCGGCCACTCGCATAGGCACCGACCGGCCCCATCGCGTAAGCTACCAGGTCGCCCACCTGCACGTTGTGccc                                                                            >  4:35989/1‑133 (MQ=255)                                                      cGCTGCGGCGGCCTCGTCCTCCAACTGAGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCAc                                                                            >  7:222981/1‑135 (MQ=255)                                                       gCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCCGCGGCTCGGCGGGCAACAGCCGGCCACTGGCATAGGCCCCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCAcc                                                                           <  7:237780/135‑1 (MQ=255)                                                        cgggggcggcCTCGACCGCCAACGGGGGCGCCCGCGGAACCCCCCGCGCGGCCGGGAACCGCCGGCCACTGGAATAGCCACCGCCCGGCCCCAGCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCAccc                                                                          <  4:16505/131‑1 (MQ=255)                                                           cggcggCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGCCCCGGTCGCCCCCCTGCACGTTGTGCACCCCAt                                                                       >  7:16588/1‑135 (MQ=255)                                                           cggcggCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCCCCCAACTGCACGTTGTGCAccccct                                                                       >  8:153393/1‑133 (MQ=255)                                                             gcggcCTCGTCCTCCACCTGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGAAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                     <  6:121855/135‑1 (MQ=255)                                                             gcggcCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                     <  2:28527/135‑1 (MQ=255)                                                               gggctggTCCTCCACCTGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACGGGCATAGGCACCTACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGcc                                                                   <  8:71496/129‑1 (MQ=255)                                                                gcCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCCCCGGCACGTTTGGCACCCCATCGcc                                                                   >  1:314261/1‑134 (MQ=255)                                                                        ctccAAGGGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACTGCCATAGCCCCCGCCCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCga                                                          <  1:243919/135‑1 (MQ=255)                                                                                  ggcggcCGCGCAACCCGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGCCACCGACCGGCCCCATCGCTAAAGCGACCCGGTCGCCCACCTGCACTTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                <  6:246320/135‑1 (MQ=255)                                                                                  ggcggcAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                <  2:45587/135‑1 (MQ=255)                                                                                  ggcggaAGCGGAACCAGCCGGTCGGCGGGCAACTCCCGCCCACTGGCTTTGCCCCCGACCGGCCCCAGCGCTTACGCGACCCGGGCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                <  6:5071/135‑1 (MQ=255)                                                                                  ggcgcaaGCGTAACAAGCCGTTGGGCCGGTAACAGCCGGCCACTGGCATAGCAACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                <  4:333169/129‑1 (MQ=255)                                                                                       cAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGCCCGGCCCCCTCGCGTACGCGACCCGGTCGCCCACCTACACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGcc                                           <  7:312053/135‑1 (MQ=255)                                                                                             acccccccGCGCGGCCGGTAACAGCCGGCCACGGGCATAGGCCCCGCCCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAg                                     <  1:102833/129‑1 (MQ=255)                                                                                             aaCCACCCGCTCGGCCGGCAACCGCGGCCAACTGCCATAGGCACCCACCGGCCCCATCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAg                                     <  2:163886/135‑1 (MQ=255)                                                                                              aCCCGCCGGTGGGCGGGTACCAGCGGGCCAGTGGCATGGGCACCGACCGGCCCCTTCGCGTCCGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGt                                    <  4:263402/135‑1 (MQ=255)                                                                                              aCCCGCCGCTCGGCCGGGACCAGCCGGCCACTGGCATAGGCACCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGt                                    <  2:101498/135‑1 (MQ=255)                                                                                               cctcccGCTCGGCCGGTAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTc                                   <  4:63980/131‑1 (MQ=255)                                                                                               ccAGCCGCTCGGCCGGGAACAGCCGGCAACTGGCATAGGCACGCACCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTc                                   <  2:314261/135‑1 (MQ=255)                                                                                                     ggggggCGGGTAACCGCCGCCCACGGGCATCGGCACCGCCCGGCCCATCCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGc                             <  3:190286/131‑1 (MQ=255)                                                                                                     gCTCGGCCGGGTACAGCCGGCCAATGGCAAAGGCACCGCCCCGCCCCATCGCGTAAGCGACCCCGTCGCCCACCTGCACCGAGTGGCCCCCATAGCCAATAGGCCACACACTGGCACCCCCTTCCAGTccccagc                             >  4:190286/1‑131 (MQ=255)                                                                                                     gCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGCCCCGGTCGCCCCCCTGCCCGTTGTGCACCCCATCGCCAATCGCCGAGACCCTGCCAGCGCCGTCCACTCCCAGGc                             >  5:90183/1‑135 (MQ=255)                                                                                                     gCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCCATGGCCGAGACCCTGCCAGAGCCTTCCAGTCCCAGGc                             >  3:57158/1‑135 (MQ=255)                                                                                                     cggcggccggcAAGCGCGGCCCAGGGGCTTCGGCCCCGCCCGCCCCCAGCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGc                             <  5:277834/132‑1 (MQ=255)                                                                                                                  cAGCCGGCCACTGGCATCGGCACCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGc                <  1:117373/135‑1 (MQ=255)                                                                                                                       ggCCTCTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAgcg           <  7:153393/135‑1 (MQ=255)                                                                                                                        gCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTc                                                                                                 <  7:208127/48‑1 (MQ=255)                                                                                                                        gCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTc                                                                                                 >  8:208127/1‑48 (MQ=255)                                                                                                                        gCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGCAGGCAATGCCAgcgc          >  7:58477/1‑135 (MQ=255)                                                                                                                        gCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAgcgc          >  4:269715/1‑135 (MQ=255)                                                                                                                          ccgtGGAATCGGCCCCGCCGGCCCCCACCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCgctg        <  6:212363/132‑1 (MQ=255)                                                                                                                          cACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCgctg        <  6:313331/135‑1 (MQ=255)                                                                                                                          cACTGCCTTAGGCCCCGCCCGGCCCCTTGGCCTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCACATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGGAATGCCAGCgctg        <  3:35989/135‑1 (MQ=255)                                                                                                                                cATAGGCACCGACCGGCCCCATCGCGTAAGCGCCCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTcccc  >  8:337003/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                ACGGCATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCC  >  KT2440/4091925‑4092186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑