Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,092,063	A→C	28.1%	V91G (GTC→GGC)	CJ019_03853 ←	2‑haloacrylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,092,063	0	A	C	28.1%	84.2 / 6.2	76	V91G (GTC→GGC)	CJ019_03853	2‑haloacrylate reductase
Reads supporting (aligned to +/- strand):  ref base A (28/24);  new base C (11/13);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTGCGCTG  >  KT2440/4091930‑4092196                                                                                                                                      |                                                                                                                                      aTAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCaccgac                                                                                                                                      <  8:237134/135‑1 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGc                                                                                                                                  >  1:200022/1‑135 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGc                                                                                                                                  >  8:164052/1‑135 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGc                                                                                                                                  >  3:333169/1‑135 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCgg                                                                                                                                   <  3:183723/134‑1 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGc                                                                                                                                  <  8:112659/135‑1 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGc                                                                                                                                  >  6:80251/1‑135 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGc                                                                                                                                  >  2:240098/1‑135 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCCGc                                                                                                                                  >  2:117373/1‑135 (MQ=255)     gtggtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTACAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGACCGGc                                                                                                                                  >  2:102833/1‑135 (MQ=255)        gtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCAGGAACAGCCGGCCACTGGCATAGGCACCGACCGGcccc                                                                                                                               >  6:305066/1‑135 (MQ=255)        gtTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCGCGTCCTCCAACAGCCGCGGCAGCGGCACCCGCCGCTCGGCCGGGCTCACCCGGCCACAGGCCTAGGCCACgccccgcccc                                                                                                                               >  4:191689/1‑135 (MQ=255)             cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTTTGCCCGGAACCGCCGGCCACAGGCATAGGCCCCCACCCGCCCCAAcgc                                                                                                                          >  4:332987/1‑135 (MQ=255)             cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGCCCCATcgc                                                                                                                          >  4:301967/1‑135 (MQ=255)             cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACGGGCATAGGCACCGACCGGcccccgcgc                                                                                                                          >  5:280716/1‑130 (MQ=255)             cAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCATCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATcgc                                                                                                                          >  8:11228/1‑135 (MQ=255)                      ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGACCAGCCGGCCACTGGCATAGGCACCGAACGGCCCCCTCGCCTAACCGAc                                                                                                                 >  1:67461/1‑135 (MQ=255)                      ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCaccga                                                                                                                                       <  4:218941/113‑1 (MQ=255)                      ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCaccga                                                                                                                                       >  3:218941/1‑113 (MQ=255)                      ttGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGCCCGGCCCCATCGCGTAAGCGAc                                                                                                                 >  5:246320/1‑135 (MQ=255)                          gcCGTGATTCCCTTGAACCGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCgg                                                                                                             <  1:67300/135‑1 (MQ=255)                          gcCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGACCCCGCCGCTCGGCCGGGCACCGCCCGCCACAGGCAAAGGCACCGCCCCGCCCCCTCGCGTAACCGcccccg                                                                                                             >  2:164490/1‑133 (MQ=255)                          gcCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCgg                                                                                                             <  7:16781/135‑1 (MQ=255)                           ccGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGCACCGCCGGCCACTGGCATAGGCCCCGCCCGGCCCCCTCGCGTAAGCGCCCCGGt                                                                                                            >  3:194802/1‑135 (MQ=255)                            cggcaTTCCCTTGAACAGCCCCGCGGCGGCGGCCTCGGCCTCCAACTCCGGCGGCAGCGCACCCAGCCGCGCGGCCGGCAACAGCCGGCCACTGGCATTGGCACCGACCGGCCCCATCGCGTACGCGACCCGGTc                                                                                                           <  8:91579/131‑1 (MQ=255)                            cGTGATTCCCTTGACCAGCCCCGCGGCGGCGGCCGCGACCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGTACCCGCGGGCCACTGGCATTGCCACCGACCGGCCCCAGCGCGTAAGCGACCCGGTc                                                                                                           <  2:268288/135‑1 (MQ=255)                            cGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTc                                                                                                           <  1:240098/135‑1 (MQ=255)                              tGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCCGCGGCAGCGGAACCAGCCGCTCGGCCGGGACCAGCCCGCCACGGGCATATGCACCGCCACGCCCCATCGCGTAACCGACCCGCTCGc                                                                                                         >  3:203041/1‑135 (MQ=255)                                 ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGGACAGCCGGCCCCTGGCATAGGCCCCGCCCGGCCCCAACGCGTAAGCGACCCCGGCGcccc                                                                                                      >  3:34692/1‑134 (MQ=255)                                 ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACCGCCGGCCACTGGCATAGGCCCCGACCGGCCCCAACCCGTAAGCGCCCCCGTCGCCCa                                                                                                      >  2:119724/1‑135 (MQ=255)                                 ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCa                                                                                                      >  4:137919/1‑135 (MQ=255)                                 ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCa                                                                                                      >  5:313331/1‑135 (MQ=255)                                 ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCCGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCCTCGCGTAAGCGCCCCCGTCGcccc                                                                                                      >  1:265903/1‑134 (MQ=255)                                 ttCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCACCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCa                                                                                                      >  4:111287/1‑135 (MQ=255)                                    ccTTGACCAGCACCGCTGCGGCGGCCTGGTCCTCCAACTGCGGCGGCAGCGGAACCCGCCGCTCGGCCGGGAACAGCGGGCCACGGGCATAGGCACCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCt                                                                                                   <  6:278006/135‑1 (MQ=255)                                    ccTTCACCAGCACCGCGGGGGCGGCCTCGCCCACCAACTGCGGCGCCAGCGGAACCCGCCGCTCGGCCGGGAACAGCCGGCCACTGCCATAGGCACCGCCCGGCCCCATCGCGAACGCGACCCGGTCGCCCACCt                                                                                                   <  6:300863/135‑1 (MQ=255)                                     cTTGAACAGCCCCGCGGGGGCGGCCTCTTCCACAACCTGGGGGGCGAGGGGAACCAGCCGCTCGGCGGGTAACAGCGGGCCAGTGGAATAGGCACAGACGGGCCCCATGGCGTAAGCGACCCGGTCGCCCACCTg                                                                                                  <  2:310699/135‑1 (MQ=255)                                           caccACCGCGGGGGCGGCCTCGTCCGCCAACGGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGCAACAGCGGGCCACTGGCATAGCCCCCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGtt                                                                                            <  5:280633/132‑1 (MQ=255)                                              cACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTtgtg                                                                                         <  4:170884/135‑1 (MQ=255)                                                 cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGCCCGGCCCCAACGCGTAAGCGCCCCGGTCGCCCCCCAGCCCCTTGGGCAc                                                                                      >  6:39039/1‑135 (MQ=255)                                                 cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGACCGGCCCCCTCGCGTAAGCGACCCGGTCGCCCCCCAGCCCGGTGGGccc                                                                                      >  2:319846/1‑133 (MQ=255)                                                 cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACCTTGTGCAc                                                                                      >  7:217787/1‑135 (MQ=255)                                                 cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGACCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCAc                                                                                      >  1:148314/1‑135 (MQ=255)                                                 cGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCCCTCGGCCGGGAACAGCCGGCCACTCGCATAGGCACCGACCGGCCCCATCGCGTAAGCTACCAGGTCGCCCACCTGCACGTTGTGccc                                                                                      >  4:35989/1‑133 (MQ=255)                                                 cGCTGCGGCGGCCTCGTCCTCCAACTGAGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCAc                                                                                      >  7:222981/1‑135 (MQ=255)                                                  gCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCCGCGGCTCGGCGGGCAACAGCCGGCCACTGGCATAGGCCCCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCAcc                                                                                     <  7:237780/135‑1 (MQ=255)                                                   cgggggcggcCTCGACCGCCAACGGGGGCGCCCGCGGAACCCCCCGCGCGGCCGGGAACCGCCGGCCACTGGAATAGCCACCGCCCGGCCCCAGCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCAccc                                                                                    <  4:16505/131‑1 (MQ=255)                                                      cggcggCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGCCCCGGTCGCCCCCCTGCACGTTGTGCACCCCAt                                                                                 >  7:16588/1‑135 (MQ=255)                                                      cggcggCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCCCCCAACTGCACGTTGTGCAccccct                                                                                 >  8:153393/1‑133 (MQ=255)                                                        gcggcCTCGTCCTCCACCTGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGAAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                               <  6:121855/135‑1 (MQ=255)                                                        gcggcCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCg                                                                               <  2:28527/135‑1 (MQ=255)                                                          gggctggTCCTCCACCTGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACGGGCATAGGCACCTACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGcc                                                                             <  8:71496/129‑1 (MQ=255)                                                           gcCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCCCCGGCACGTTTGGCACCCCATCGcc                                                                             >  1:314261/1‑134 (MQ=255)                                                                   ctccAAGGGCGGCGGCAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACTGCCATAGCCCCCGCCCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCga                                                                    <  1:243919/135‑1 (MQ=255)                                                                             ggcggcCGCGCAACCCGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGCCACCGACCGGCCCCATCGCTAAAGCGACCCGGTCGCCCACCTGCACTTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                          <  6:246320/135‑1 (MQ=255)                                                                             ggcggcAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                          <  2:45587/135‑1 (MQ=255)                                                                             ggcggaAGCGGAACCAGCCGGTCGGCGGGCAACTCCCGCCCACTGGCTTTGCCCCCGACCGGCCCCAGCGCTTACGCGACCCGGGCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                          <  6:5071/135‑1 (MQ=255)                                                                             ggcgcaaGCGTAACAAGCCGTTGGGCCGGTAACAGCCGGCCACTGGCATAGCAACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCa                                                          <  4:333169/129‑1 (MQ=255)                                                                                  cAGCGGAACCAGCCGCGCGGCCGGGAACAGCCGGCCACTGGCATAGGCCCCGCCCGGCCCCCTCGCGTACGCGACCCGGTCGCCCACCTACACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGcc                                                     <  7:312053/135‑1 (MQ=255)                                                                                        acccccccGCGCGGCCGGTAACAGCCGGCCACGGGCATAGGCCCCGCCCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAg                                               <  1:102833/129‑1 (MQ=255)                                                                                        aaCCACCCGCTCGGCCGGCAACCGCGGCCAACTGCCATAGGCACCCACCGGCCCCATCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAg                                               <  2:163886/135‑1 (MQ=255)                                                                                         aCCCGCCGGTGGGCGGGTACCAGCGGGCCAGTGGCATGGGCACCGACCGGCCCCTTCGCGTCCGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGt                                              <  4:263402/135‑1 (MQ=255)                                                                                         aCCCGCCGCTCGGCCGGGACCAGCCGGCCACTGGCATAGGCACCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGt                                              <  2:101498/135‑1 (MQ=255)                                                                                          cctcccGCTCGGCCGGTAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTc                                             <  4:63980/131‑1 (MQ=255)                                                                                          ccAGCCGCTCGGCCGGGAACAGCCGGCAACTGGCATAGGCACGCACCGGCCCCATCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTc                                             <  2:314261/135‑1 (MQ=255)                                                                                                ggggggCGGGTAACCGCCGCCCACGGGCATCGGCACCGCCCGGCCCATCCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGc                                       <  3:190286/131‑1 (MQ=255)                                                                                                gCTCGGCCGGGTACAGCCGGCCAATGGCAAAGGCACCGCCCCGCCCCATCGCGTAAGCGACCCCGTCGCCCACCTGCACCGAGTGGCCCCCATAGCCAATAGGCCACACACTGGCACCCCCTTCCAGTccccagc                                       >  4:190286/1‑131 (MQ=255)                                                                                                gCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGCCCCGGTCGCCCCCCTGCCCGTTGTGCACCCCATCGCCAATCGCCGAGACCCTGCCAGCGCCGTCCACTCCCAGGc                                       >  5:90183/1‑135 (MQ=255)                                                                                                gCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCCATGGCCGAGACCCTGCCAGAGCCTTCCAGTCCCAGGc                                       >  3:57158/1‑135 (MQ=255)                                                                                                cggcggccggcAAGCGCGGCCCAGGGGCTTCGGCCCCGCCCGCCCCCAGCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGc                                       <  5:277834/132‑1 (MQ=255)                                                                                                             cAGCCGGCCACTGGCATCGGCACCGCCCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGc                          <  1:117373/135‑1 (MQ=255)                                                                                                                  ggCCTCTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAgcg                     <  7:153393/135‑1 (MQ=255)                                                                                                                   gCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTc                                                                                                           <  7:208127/48‑1 (MQ=255)                                                                                                                   gCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTc                                                                                                           >  8:208127/1‑48 (MQ=255)                                                                                                                   gCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGCAGGCAATGCCAgcgc                    >  7:58477/1‑135 (MQ=255)                                                                                                                   gCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAgcgc                    >  4:269715/1‑135 (MQ=255)                                                                                                                     ccgtGGAATCGGCCCCGCCGGCCCCCACCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCgctg                  <  6:212363/132‑1 (MQ=255)                                                                                                                     cACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCgctg                  <  6:313331/135‑1 (MQ=255)                                                                                                                     cACTGCCTTAGGCCCCGCCCGGCCCCTTGGCCTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCACATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGGAATGCCAGCgctg                  <  3:35989/135‑1 (MQ=255)                                                                                                                           cATAGGCACCGACCGGCCCCATCGCGTAAGCGCCCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTcccc            >  8:337003/1‑135 (MQ=255)                                                                                                                               gcccccgaccgCCCCCACCGCGTACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCtt        <  7:291170/131‑1 (MQ=255)                                                                                                                                     gaccgGCCCCATCCCGAAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTGCGCTg  <  1:319846/135‑1 (MQ=255)                                                                                                                                     gaccgGCCCCACCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTGCGCTg  <  2:69060/135‑1 (MQ=255)                                                                                                                                     gaccgCCCCCATCGCGAACGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTGCGCTg  <  3:111287/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      ATAGGTGGTTTTCAACAGATATTGCGCCGTGATTCCCTTGAACAGCACCGCTGCGGCGGCCTCGTCCTCCAACTGCGGCGGCAGCGGAACCAGCCGCTCGGCCGGGAACAGCCGGCCACTGGCATAGGCACCGACCGGCCCCATCGCGTAAGCGACCCGGTCGCCCACCTGCACGTTGTGCACCCCATCGCCAATGGCCGAGACCCTGCCAGCGCCTTCCAGTCCCAGGCCTGAAGGCAATGCCAGCGCTGCTCCCCCCTTGCGCTG  >  KT2440/4091930‑4092196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑