Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,127,172 T→G 10.1% T148P (ACC→CCC)  nrtD_3 ← Nitrate import ATP‑binding protein NrtD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,127,1720TG10.1% 94.1 / 2.2 51T148P (ACC→CCC) nrtD_3Nitrate import ATP‑binding protein NrtD
Reads supporting (aligned to +/- strand):  ref base T (18/26);  new base G (2/4);  total (20/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

TTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGC  >  KT2440/4127045‑4127298
                                                                                                                               |                                                                                                                              
ttGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCCCCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTgg                                                                                                                         >  4:201757/1‑135 (MQ=255)
    gCGTGGCGATCAGTTGCAGCAGCAAGTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCa                                                                                                                     <  7:102104/135‑1 (MQ=255)
           gATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCCCCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATgccgcc                                                                                                              >  8:55176/1‑135 (MQ=255)
           gATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCACCAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCCCCCCGGGGCGCGGGGCCCAgccgcc                                                                                                              >  1:168864/1‑135 (MQ=255)
           gATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATgccg                                                                                                                >  5:125663/1‑133 (MQ=255)
           gATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATgccg                                                                                                                >  4:212301/1‑133 (MQ=255)
                gtggccgcagcaACTGCTGCATGTCGGCGCGGGTCACCTCAACCACCGCACCGAAGGGTTCATCCAGCAACACGACTTCGGGCGGGCGTGCCAGGCTGCGGGCCAGGGCGGTGCGCTGGGCCATGCCGCCAGACa                                                                                                         <  5:59116/129‑1 (MQ=255)
                gtggcagcagcaACTGCTGCAGGTCGGCGCGGGTCACCTCATCCAACCCCCCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACa                                                                                                         <  2:65221/132‑1 (MQ=255)
                gagccagcagcaACTGCGGCATGGCGGCGCGGGGCACAGCATCCACCGCCCCGACGGTTGCATCCAGCAACAACCCTACGGGCGGGCGTGCCAGGCCGCGGGCCAGCGCGGGGCGCGGGGCCATGCCGCCAGACa                                                                                                         <  2:141554/131‑1 (MQ=255)
                    cagcagcaACTGCTGAATGTCGGCGCGGGTCACCTCATCCAACGCACCGACGGGTTCATCCAGCAACAATACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGTGCCATGCCGCCAGACAACt                                                                                                      <  7:228274/134‑1 (MQ=255)
                          caACTGCTGCATGGCGGCGCGGGGCACCTAAACCAACGCACCGAAGGGTGCATCCAGCAACAAGACTGCGGGCTGGCGTCCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCgg                                                                                               <  2:316589/135‑1 (MQ=255)
                                 tgcATGTCGGCGGGGGTCACCTCATCCAACCCACCGAAGGGTTCAGCCAGCAACAAGACTGCGGGCTGGCGGGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTgg                                                                                        <  1:62294/135‑1 (MQ=255)
                                 tgcATGGCGGCGCGGGTCACCTCACCCACCGCACCGAAGGTTTCATCCAGCAACAAGACTTCGGGCTGGGGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTgg                                                                                        <  6:239725/135‑1 (MQ=255)
                                      gTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTgc                                                                                                    <  8:317984/118‑1 (MQ=255)
                                      gTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTgc                                                                                                    >  7:317984/1‑118 (MQ=255)
                                        cGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCCGCGCGGTGCGCTGGGCCATGCCGCCAGCCCACTGCGCCGGGTGCTGGCCACggg                                                                                 >  5:178522/1‑135 (MQ=255)
                                          gcgcGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCCGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCCGCGCGGGGCGCTGGGCCCTGCAGCCACCCCACAGCCCCGGGGTCTGGGCACCGGCg                                                                               >  6:284498/1‑135 (MQ=255)
                                              gggTCACCTCATCCAACGCACCGACGGGTTCAACCACCAACACGACTTCGGGCTGGCGGGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTggg                                                                           <  1:241329/135‑1 (MQ=255)
                                              gggTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCCGCGCGGTGGGGTGGGCCCAGGCCCCCGCCAACTGCGCCGGGTACTGGCCACGGGCGgggg                                                                           >  6:13164/1‑135 (MQ=255)
                                              gggCCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTggg                                                                           <  6:237657/135‑1 (MQ=255)
                                                    ccTCATCCACCGCCCCCACGGGTTCAGCCAGCAACACGACTGCGGGCGGGCGGGCCAGGCAGCGGGCCAGCGCGGGGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGc                                                                     <  6:37502/135‑1 (MQ=255)
                                                    ccTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGc                                                                     <  6:157922/135‑1 (MQ=255)
                                                        aaccACCGCACCGACGGGTCCATCCCGCAACACGACTGCGGGGGGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCcac                                                                 <  5:90412/133‑1 (MQ=255)
                                                           cAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCCCCGCGGTGCGCTGGGCCATGCCGCCCGCCAACTGCGCCGGGTACGGGCCCCGGGCCTGGGGCCGCCCcacccc                                                              >  6:10697/1‑132 (MQ=255)
                                                           cAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCCGGCCcacccc                                                              >  8:239588/1‑132 (MQ=255)
                                                                  cggAAGGGTTAATCAAGCACCAAGACTTCGGGCTGGGGTGCCAGGCAGCGGGCCCGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATc                                                       <  5:177847/133‑1 (MQ=255)
                                                                  ccGAAGGGTTCATCCAGCAACATGACTTCGGGGTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATc                                                       <  8:165381/135‑1 (MQ=255)
                                                                  ccGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATc                                                       <  2:101495/135‑1 (MQ=255)
                                                                  ccGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATc                                                       <  6:125663/135‑1 (MQ=255)
                                                                  ccGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATc                                                       <  2:97048/135‑1 (MQ=255)
                                                                  ccGAAGGGTTCATCCAGCAACAAGACTTCGGGCGGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATc                                                       <  4:192317/135‑1 (MQ=255)
                                                                  ccGAAGGGGTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCCGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATc                                                       <  3:201757/135‑1 (MQ=255)
                                                                      aGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCACAAAAGCAt                                                   >  4:165647/1‑135 (MQ=255)
                                                                         gTTCATCCCGCAACAAGACTTCGGGCGGGCGGGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGt                                                <  4:232502/135‑1 (MQ=255)
                                                                         gTTCATCCAGCAACAAGACTGCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGt                                                <  7:322923/135‑1 (MQ=255)
                                                                                cAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTCTGCCGGGTACTGGCCACCGGCGTTGGCCAGGCCCCCAGCCACAATAGCATGGTCGAAGCg                                         >  7:239488/1‑135 (MQ=255)
                                                                                cAGCAACAAGACTTCGGGCTGGCGTGCAAGGCAGCGTGCCACCCCCGTGCGCTGGGCCAAGCCCCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCGCAATAGCATGGTCGATGCg                                         >  4:165419/1‑135 (MQ=255)
                                                                                   caacacGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGGGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGc                                      <  4:327152/135‑1 (MQ=255)
                                                                                      caaGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGCCAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCCCCGCCGCAATCGCATGGTCGATGCGTGGACg                                   >  5:118031/1‑135 (MQ=255)
                                                                                      caaGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACAGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAAAAGCAAGGTCCATTCGTTCACg                                   >  8:322923/1‑135 (MQ=255)
                                                                                       aaGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCCCCCCCGCAATCGCATGGTCGATGCGTGCCCgg                                  >  3:35137/1‑135 (MQ=255)
                                                                                        cgACTGCGGGCGGGCGGGCCAGGCAGCGGGCCAGCGCGGGGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGc                                 <  5:21421/134‑1 (MQ=255)
                                                                                            ttCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCCGGCCCCCCGCCTCAATCGCCTGGTCGCTGCGGGCACGGCggtg                             >  6:132739/1‑132 (MQ=255)
                                                                                                 gcgggCGGGCCAGGCAGCGGGCCCGCGCGGGGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCAt                        <  6:273005/132‑1 (MQ=255)
                                                                                                 gCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCAt                        <  3:165647/135‑1 (MQ=255)
                                                                                                 gCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCACACAACAGCGCCGGGTACAGGCCCCGGGCGTGGGCCACGCCCACAGCCGCAATCGCATGGTGCCTGCGGGCAAGGCGGTCGGCaa                        >  4:227826/1‑134 (MQ=255)
                                                                                                               cAGCGGGCCCGCGGGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGc          <  1:197017/135‑1 (MQ=255)
                                                                                                               cAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGc          <  7:55176/135‑1 (MQ=255)
                                                                                                                    ggCCGGGGCGGCGGGCGGGGCCCGGCCGCCAGACAACTGCGCCGGGTACGGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACg     <  1:162206/135‑1 (MQ=255)
                                                                                                                     gCCCGCGCGGTGCGCGGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGGGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACgg    <  5:37091/135‑1 (MQ=255)
                                                                                                                       cAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCCCCGCCGCAATCGCATGGTCGATGCGTGCCCGGCGTTCGGCCTCCGCCCGCCGGGGCTGACGGGc  >  7:64880/1‑135 (MQ=255)
                                                                                                                               |                                                                                                                              
TTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGC  >  KT2440/4127045‑4127298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑