Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,131,763	T→G	27.6%	E23A (GAG→GCG)	CJ019_03889 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,131,763	0	T	G	27.6%	60.8 / 2.6	55	E23A (GAG→GCG)	CJ019_03889	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (16/20);  new base G (15/2);  total (33/22)
Fisher's exact test for biased strand distribution p-value = 2.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACGATGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGC  >  KT2440/4131629‑4131897                                                                                                                                       |                                                                                                                                       gCACGATGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCt                                                                                                                                        <  3:282905/135‑1 (MQ=255)         gCGATCAGGCCCCACAACAGGTTCCGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                <  4:187537/135‑1 (MQ=255)         gCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                <  7:319860/135‑1 (MQ=255)         gCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                <  5:165755/135‑1 (MQ=255)          cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCTCCGGTCACGATAGGCAGTGGCGCGATGTAGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGACCCCTGGCGCTCGGCAGGTg                                                                                                                               >  3:307901/1‑135 (MQ=255)          cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCCTGGCGCTCGGCCgggg                                                                                                                               >  5:143435/1‑133 (MQ=255)          cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                <  1:177094/134‑1 (MQ=255)          cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                >  5:157369/1‑134 (MQ=255)          cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCAAGGCGCGCCGCAgggg                                                                                                                               >  7:105380/1‑133 (MQ=255)          cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCCTGGCGCGCGGcctggg                                                                                                                               >  2:186460/1‑130 (MQ=255)          cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCCTGGCGCGCGGCCGGTg                                                                                                                               >  4:195373/1‑135 (MQ=255)          cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCCTCCCAGGGCGGACGGCAGGTg                                                                                                                               >  7:160742/1‑135 (MQ=255)           gATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCCTGGCGCGCGGCCGGTgg                                                                                                                              >  6:267524/1‑135 (MQ=255)                        aacaGGGTCAGGTGTAACACGTGGACCACCTTGGCGCCGGTCACGATAGGCAGGGGGGCGTTGTTGGTACTGACCCAGTGGGTCAACAGGTCGCGCGCCTTCCCTTGGCGCTGGGCTGGTGGAACGTAATCGAcc                                                                                                                 <  6:252839/135‑1 (MQ=255)                              ttCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCCTGGCGCTCGGCAGGTGGATCGTAATCGCCCGTGTGa                                                                                                           >  8:220931/1‑135 (MQ=255)                                cAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTGCCCCTGGCGCGCGGCAGGGGGCACGTAATAGACCGGGTGAtt                                                                                                         >  2:175667/1‑135 (MQ=255)                                cAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTGCCCTGGCGCGCGGCAGGTGGGACGGAATCGGCCGGGTGCtt                                                                                                         >  5:45971/1‑135 (MQ=255)                                cAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGCGCCGCCGGGGGCACGTAATCGCCCgtgggttt                                                                                                         >  2:235456/1‑130 (MQ=255)                                cAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGAAAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCCTGGGGCTGGGCCCGGGGTCCGGAATCGGCCgtgggcgt                                                                                                         >  8:89463/1‑130 (MQ=255)                                  ggTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGCTCGGGAGGGGGAACGTAATAGACCGTGTGATtct                                                                                                       >  8:34098/1‑135 (MQ=255)                                  ggTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGCGGGGCCGGGGGGAAGTTATCGCCCGGGTGCttgg                                                                                                       >  7:315160/1‑133 (MQ=255)                                     ggAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGCTCGGCAGGTGGCACGTAATCGACCGTGTGATTGTCg                                                                                                     >  7:257375/1‑134 (MQ=255)                                         cacCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCGGGGGAGGGGGGACGTAATCGCCCGGGTGTTTCTCGGCaaa                                                                                                >  1:6853/1‑135 (MQ=255)                                                   cccttGGCGCCGGGCACGATAGGCAGTGGCGCGAGGTGGGTACGGAACGCGAGGGTGACCAGGGCGCGCGCCTTCCCTTGGGGGTCGGCTGGTGGACCGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGc                                                                                      <  8:294609/131‑1 (MQ=255)                                                    ccctGGCGCCGGGCACGATCGGCTGGGGGGCGTTGTGGGTACTGACCCTGCGGGGCAACCGGTCGCGGGCCTTCCCTTGGGGCGCGGCTGGGGGAAGTTAATCGACCGGGTGATTCTCGACAAACTGGTGTTGcg                                                                                     <  8:165939/132‑1 (MQ=255)                                                    ccaTGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCCTGGCGCGCGGCAGGTGGAACGTAATCGACCGTGTGTTTGTCGACAAACTGGTGTTggg                                                                                     >  1:278523/1‑133 (MQ=255)                                                     caTGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGcga                                                                                    <  2:200624/135‑1 (MQ=255)                                                     caTGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGcga                                                                                    <  4:305532/135‑1 (MQ=255)                                                       tGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCAGGGCGCACGGCAGGTGGCACGTAATAGACCGTGTGATTCTCGACAAACTGGTGGTGcgacg                                                                                  >  5:151808/1‑135 (MQ=255)                                                            ccGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCAAGGGGCGCGGCAGGTGGTACGTAATCCACCGGGGGATTATGGCCAAACTGGTGGTGGGACGCCggg                                                                             >  1:289723/1‑134 (MQ=255)                                                                  aCGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTc                                                                                                                                       >  7:116277/1‑71 (MQ=255)                                                                  aCGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTc                                                                                                                                       <  8:116277/71‑1 (MQ=255)                                                                  aCGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCGCGGCAGGTGGAACGGTATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGAc                                                                       >  1:234410/1‑135 (MQ=255)                                                                  aCGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTGCCCATGGCGCGCGGCCGGTGGAAAGGAATCCACCCGGGGAGTCTCGCCAAACGGGTGTTGCGACGCCCGGTGGGAc                                                                       >  7:165939/1‑135 (MQ=255)                                                                                cgcgATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCCTGGCGCGCGGCAGGTGGAACGTAATCGACCCGGTGGTTCTCGCCAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCt                                                          >  1:12225/1‑134 (MQ=255)                                                                                 gcgATGTTGGTACTGACCCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGGGCGGCAGGTGGAACGTAATCGACCGTGTGATTAACGCAAAACTTGTGTTGCGACGCCGGTTGGGCCATGGTTTTGAGCTc                                                         >  8:17097/1‑134 (MQ=255)                                                                                 gcgATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCt                                                                                                                                        >  8:12590/1‑55 (MQ=255)                                                                                 gcgATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCt                                                                                                                                        <  7:12590/55‑1 (MQ=255)                                                                                   taTGGTGGTACGTACCCAGCGGGTGACCGGGGGGCGGGCCTCCCCCTGGGGCGCGGCTGGTGGAACGTACTCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGaa                                                      <  1:154042/134‑1 (MQ=255)                                                                                       ttGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGCCCGTGTGATTCTAGACAAACTGGGGTTGCGACGACGGTTGGGTCATGGTTTTCAGCTCGAAAGGt                                                  >  7:49632/1‑135 (MQ=255)                                                                                       ttGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGt                                                  >  7:320959/1‑135 (MQ=255)                                                                                                  ccAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCt                                       >  1:4463/1‑135 (MQ=255)                                                                                                        ggTGAACAGGTCGCGCGCCGTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGGTGGGACATGGTTTTCAGCTCGAAAGGTCGTGTTTTTGTTTTTaa                                 >  5:307695/1‑135 (MQ=255)                                                                                                               aGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCCTGTGATTATCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGGAAGGTCGTTTTTATTGTTGTTAggtgacc                          >  3:193216/1‑130 (MQ=255)                                                                                                                   cgcgcgCCTTCCCATGGCGCTCGGCAGGTGGAAAGTAATCGACCGTGTGATTCTCGCCAAACTGGTGTTGCGCCGACGGGTGGGGCATGGTTTTGAGCTCGAAAGGTCGTTTTTATATTTTTTATGGAACCGcc                       >  2:212563/1‑133 (MQ=255)                                                                                                                      gcgcCTTCCCATGGCGCTCGGCAGTTGGAAAGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  7:220931/135‑1 (MQ=255)                                                                                                                      gcgcCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  5:231928/135‑1 (MQ=255)                                                                                                                         ccTTCCCATGGCGCTCGGCAGGGGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAggc                <  6:45971/135‑1 (MQ=255)                                                                                                                          cTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTTTCTTTGTTATGAAACCGCATCAAggcg               >  2:27425/1‑135 (MQ=255)                                                                                                                           ttCCCAGGGCGCTCGGCAGGGGGAACGTACTCGACCGTGGGATTCTCGACAAACTGGTGTTGCGTCGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAggcgg              <  7:89463/135‑1 (MQ=255)                                                                                                                              ggttGGCGGTGGGCTGGTGGAACGACATCGACCGTGTGATTCTGGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAATGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGcc            <  3:249525/131‑1 (MQ=255)                                                                                                                              ccATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGcc            <  1:205029/134‑1 (MQ=255)                                                                                                                              ccATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTGTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGcc            <  8:174844/134‑1 (MQ=255)                                                                                                                                   gcgcTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCa       <  1:186460/134‑1 (MQ=255)                                                                                                                                     gcTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCt    <  7:41531/135‑1 (MQ=255)                                                                                                                                     gcTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCt    <  7:34098/135‑1 (MQ=255)                                                                                                                                     gcTCGGCAGGGGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCt    <  1:12335/135‑1 (MQ=255)                                                                                                                                      cTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTGTTTTTGTTTGTAAGGAAACCGCATAAAGGCGGCCTCTCAGCTg   >  1:226343/1‑135 (MQ=255)                                                                                                                                       tCGGCAGTTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  8:244510/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  4:47877/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  8:154861/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  1:27425/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  7:197751/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  1:175667/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  8:320959/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGTGGAACGTAATCGACCGTGGGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  2:289723/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGGGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  5:147373/135‑1 (MQ=255)                                                                                                                                       tCGGCAGGGGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  2:278523/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GCACGATGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGACGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGC  >  KT2440/4131629‑4131897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑