Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 169,811 C→A 10.5% intergenic (+775/+2912) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440169,8110CA10.5% 162.1 / 12.6 67intergenic (+775/+2912)PP_RS08750/fhuA_1tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
Reads supporting (aligned to +/- strand):  ref base C (30/30);  new base A (5/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAGGG  >  KT2440/169684‑169919
                                                                                                                               |                                                                                                            
cATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGCAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTg                                                                                                       >  7:210805/1‑135 (MQ=17)
           gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc                                                                                                              <  4:165767/117‑1 (MQ=32)
           gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc                                                                                                              >  5:307193/1‑117 (MQ=32)
           gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc                                                                                                              >  3:165767/1‑117 (MQ=32)
           gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc                                                                                                              <  6:302172/117‑1 (MQ=32)
           gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc                                                                                                              <  6:307193/117‑1 (MQ=32)
           gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGcc                                                                                                              >  5:302172/1‑117 (MQ=32)
           gCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg                                                                                            <  2:210359/135‑1 (MQ=32)
             tAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGc                                                                                           >  5:134104/1‑134 (MQ=32)
             tAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCg                                                                                          <  6:154298/135‑1 (MQ=32)
                atcctactGCCTGACCGATAGTGAACCAGTACCGCGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc                                                                                       <  6:280749/132‑1 (MQ=12)
                aTACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc                                                                                       <  4:207951/135‑1 (MQ=32)
                 tactacTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg                                                                                            <  5:28054/129‑1 (MQ=32)
                 tactacTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTg                                                                                            >  6:28054/1‑129 (MQ=32)
                  actcctGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCt                                                                                     >  3:238571/1‑135 (MQ=11)
                  actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc                                                                                       <  1:56054/133‑1 (MQ=32)
                  actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTAc                                                                                       >  2:56054/1‑133 (MQ=32)
                  actactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCt                                                                                     >  8:321142/1‑135 (MQ=32)
                    tactGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCtt                                                                                    <  8:210805/134‑1 (MQ=32)
                       tgactgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGt                                                                                >  8:190482/1‑135 (MQ=11)
                         actgacCGATAGTGAACCAGTACCGTGAGTGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGAGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta                                                                               >  2:67658/1‑134 (MQ=17)
                         actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta                                                                               >  5:72275/1‑134 (MQ=32)
                         actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta                                                                               >  7:248535/1‑134 (MQ=32)
                         actgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta                                                                               >  1:218448/1‑134 (MQ=32)
                          ctgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGta                                                                               <  6:313036/133‑1 (MQ=32)
                            gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAAt                                                                           >  5:224474/1‑135 (MQ=32)
                                gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATggg                                                                        >  4:283804/1‑134 (MQ=11)
                                 aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAACGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGATGACTGCGTACCTTTTGTATAATGGGTc                                                                      <  7:130028/135‑1 (MQ=17)
                                 aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                      <  4:159365/135‑1 (MQ=32)
                                      gAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGa                                                                 <  7:48781/135‑1 (MQ=32)
                                          cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACtt                                                              >  4:31860/1‑134 (MQ=32)
                                          cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACt                                                               <  4:69643/133‑1 (MQ=32)
                                          cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACt                                                               >  3:69643/1‑133 (MQ=32)
                                             tACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat                                                          <  5:34944/135‑1 (MQ=32)
                                               ccGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTGAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTc                                                        >  2:77657/1‑135 (MQ=11)
                                               ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAGCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat                                                          >  2:291796/1‑133 (MQ=17)
                                               ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat                                                          >  8:132899/1‑133 (MQ=32)
                                                  tGAGGGAAAGGCTAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGt                                                     <  3:204537/135‑1 (MQ=11)
                                                  tGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGt                                                     <  7:190482/135‑1 (MQ=11)
                                                     gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTgg                                                   >  8:218060/1‑134 (MQ=32)
                                                     gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                  <  2:54530/135‑1 (MQ=32)
                                                     gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                  >  4:312296/1‑135 (MQ=32)
                                                       gAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGcaa                                                >  2:191542/1‑133 (MQ=11)
                                                        aaaGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAg                                               <  7:328657/135‑1 (MQ=32)
                                                        aaaGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAg                                               <  7:132899/135‑1 (MQ=32)
                                                          aGGCGATAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTAGAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt                                             <  2:143552/135‑1 (MQ=17)
                                                             cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt                                                                                             <  7:35371/84‑1 (MQ=33)
                                                             cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACt                                                                                             >  8:35371/1‑84 (MQ=33)
                                                             cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCtttt                                                                                  >  3:195903/1‑95 (MQ=33)
                                                             cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCtttt                                                                                  <  4:195903/95‑1 (MQ=33)
                                                                         cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt                                             <  3:145577/120‑1 (MQ=32)
                                                                         cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt                                             >  4:145577/1‑120 (MQ=32)
                                                                         cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGa                              >  4:16208/1‑135 (MQ=32)
                                                                                   aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGTGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa                    <  1:139020/135‑1 (MQ=17)
                                                                                   aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt                                             <  3:103198/110‑1 (MQ=32)
                                                                                   aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCt                                             >  4:103198/1‑110 (MQ=32)
                                                                                   aGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGGTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa                    <  6:158476/135‑1 (MQ=17)
                                                                                   aGTGAAATAGAACCTGAAACCGTGGGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGa                    <  1:298720/135‑1 (MQ=17)
                                                                                     tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa                  <  8:338131/135‑1 (MQ=32)
                                                                                     tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa                  <  3:55082/135‑1 (MQ=32)
                                                                                     tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa                  <  5:270899/135‑1 (MQ=32)
                                                                                     tGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGaaa                  <  2:218448/135‑1 (MQ=32)
                                                                                      gAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAg                 >  8:102017/1‑135 (MQ=32)
                                                                                        aaTAGAACCTGAAACCGTGTGCGAACAAGCAGTGGGAGCCTACTTTGTTTGGTGACTGCGTAGCGTTAGTATAATGCGTCAGCGACTGATAATCAGTGGAGAGATTAACAGAATAGGGGAGGCGTAGCGAAAg                 >  2:143877/1‑133 (MQ=11)
                                                                                             aaCCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTc           >  6:247808/1‑134 (MQ=32)
                                                                                               ccTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTa        <  3:31860/135‑1 (MQ=32)
                                                                                                cTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTa        >  8:236368/1‑134 (MQ=32)
                                                                                                cTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTa        >  4:225902/1‑134 (MQ=32)
                                                                                                cTGAAACCGTGTGCGTACAAGCACTGGTAGCCTACTTTGTTTGGTGAATGCGTAGCTTATGTATAATCCCTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAATCGAGACTTa        >  1:75715/1‑134 (MQ=11)
                                                                                                  gAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATa     >  1:231196/1‑135 (MQ=32)
                                                                                                     aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                      <  7:73870/67‑1 (MQ=35)
                                                                                                     aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                      >  8:73870/1‑67 (MQ=35)
                                                                                                     aCCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAggg  >  1:86623/1‑135 (MQ=32)
                                                                                                                               |                                                                                                            
CATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTTTGTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAGGG  >  KT2440/169684‑169919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: