| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,135,607 | A→C | 29.7% | Q32P (CAG→CCG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,135,607 | 0 | A | C | 29.7% | 39.6 / 6.6 | 59 | Q32P (CAG→CCG) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (24/14); new base C (14/5); total (38/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
AAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTG > KT2440/4135473‑4135740 | acgcccgcccCCTCACCGAATCCGCCAGGGGGATGGTTGCATGACCCTCGTTCGTTTCCAGATGGGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 4:148014/133‑1 (MQ=255)aaGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGGTCGTTTCCAGATGGGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 4:81414/135‑1 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCt > 8:275982/1‑135 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCt > 7:320391/1‑135 (MQ=255) cccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGACTCAACCCGGCCACCGCCCCCGCCTGGgc > 2:285172/1‑135 (MQ=255) ccGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCACGGCGCCACt > 6:325062/1‑135 (MQ=255) gAATCCGCCAAGGGGATTGTTGCATGACCCTCATTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGc > 8:69807/1‑135 (MQ=255) tCCCCCAAGGGGATTGTTGCATGACCCTCGTGGGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGCCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCg < 4:35408/135‑1 (MQ=255) ccGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCCACTGCCCgc > 3:56271/1‑134 (MQ=255) tgttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCACCTGCCCGACGCCGCGGAAGcc > 4:102293/1‑135 (MQ=255) gttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCCCCTGCCCGACGCCGCTGAAGcc > 7:31653/1‑134 (MQ=255) ttgCATGACCCTGGTGCGTTCCCAGATGTGCATCGACGGCCAATGGCGCGAGGCCCAGAGCGGCAAGACCTTCGACACCCTCACCCCGGCCCCCGCCCTGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCg < 1:277636/134‑1 (MQ=255) ttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCTGGGCCCAACTGCCCCACGCCGATCGAGCCGa > 2:214459/1‑135 (MQ=255) gACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCCCCAGCCCGACGCCCCTGAAGCCCAAGTggc > 5:254519/1‑134 (MQ=255) gACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTggc > 8:314196/1‑134 (MQ=255) ccgtggttcgttTCCAGATGTGTAGCGCCGGCGATTGGCGCGAGGCCCTGAGCGCCATGACCTGCGACCGCCTCACCCCGGCACCCGCCCCGGGCTGGGCGCTACTGCCCGACGCCGATGAAGCCGATGTGGAGc < 2:184212/130‑1 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCTGGGCGCCCCAGCCCGACGCCGATGAAGCCCAAGTGGAGc > 7:197113/1‑135 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCGGGGCGCACCAGCCCCCCCCCGACGAAGCCGAAGTGGAGc > 3:224859/1‑135 (MQ=255) cccTCGTGCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAg < 8:320391/134‑1 (MQ=255) tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACAGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGt > 7:333984/1‑135 (MQ=255) tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCCACTGCCCCACGCCGAACAACCCCATGTGGAGCTGGCCga > 2:232416/1‑134 (MQ=255) gttTCCAGAGGGGCAGCGACGGCCAATGCCGCGAGGCCCAGAGCGGCAACACCTTCCACACCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTc < 2:44777/135‑1 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCACTGCCCCACGCCCAGGCAGCCGCTGTGGAGCAGGCCGTTccgcc > 8:24991/1‑131 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACACGGCCCCCGCCCAGGCCTGGGCGCAACTGCCGGCCGCCGATGACCCCGCTGTGGAGCTGGCCGATcccgc > 6:178526/1‑131 (MQ=255) cAGATGTGCATCGCCGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCCGGGCGCCACAGCCCGACGCCCCTGAAGCCGAGGTGGAGCTGGCCGGTCACgcc > 1:199519/1‑135 (MQ=255) cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGACTCAACCCGGCCACCGCCAAGCCCACGGCGCCACAGACCCACCCCGCAGAAGCCGATGTGGAGCTGGCCCTTAAGgcc > 5:5048/1‑135 (MQ=255) aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGACGCCGCTGAAGCCGCTGTGGAGCTGGCCGGTCAGGCCGccccccgt > 1:33798/1‑130 (MQ=255) aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCCCGGCCCGCCGCCGCGGCAGCCGCGGTGGAGCGGGCCGGTCAGGCCGCCCcgcgg > 1:13173/1‑134 (MQ=255) ggccGGCCCATGGCGCGCTGCCCAGAGCGCCAAGACCTTCGACAGCAGCACCCCGGCCCCCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGAGGACGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGc < 4:246329/132‑1 (MQ=255) cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCCACTGCCCGCCGCCCATGAAGCCGAGGTGGAGCTGGCCGGTCAGGCCCCCCCGCGTGc > 3:87351/1‑135 (MQ=255) cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCAACAGCCCGCCGCCGCTGATCCCCAAGTGGAGCAGGCCGATCACGCCGCCCcgcgagg > 1:254932/1‑132 (MQ=255) cgcccAATCGCGCTTGGCCCAGAGCGGCACCCCCTTCGACACCCTCACCCCGGCCCCCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCtt < 8:292519/132‑1 (MQ=255) gCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCGGCCAGGGGGCCCCGGCCCCCCGCCGCTGCAGCGGGGGTGGAGCGGGCCGGTCAGGCCCCCCCGCGGGccggcg > 5:337005/1‑131 (MQ=255) aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa > 8:69893/1‑135 (MQ=255) aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa > 8:167405/1‑135 (MQ=255) cgcgATGCCCAGAGCGCCACGACCTTCGACCGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg < 3:6476/135‑1 (MQ=255) cgcgAGGCCCCGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg < 6:95015/135‑1 (MQ=255) gATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCg < 8:94456/135‑1 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGCCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 3:205060/135‑1 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGGCGGGGGGGCACGGCCCGCCGCCCCTGAAGCCGCAGTGGGGCTGGCCGTTCAGGCCGCCCCGCGGGCCGGCGCCAGCAACGCGTg > 7:337762/1‑135 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg > 1:273259/1‑135 (MQ=255) agCCCAGAGCGCCAAGACCTCCGACACCCTCACCCCGGCCACCGCCACTGCCTGGGCGCAACTGCCCGACGCCGAAGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 3:201021/134‑1 (MQ=255) agagCGGCAAGCCCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGCCGCCGCTGAAGCCGCTGTGGAGCTGGCCGGTCCGGCCGCCCCCCGGGCCGTCGCCAGGCAGGCGGGGCgca > 1:126108/1‑135 (MQ=255) agagCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGGACCAGCCCGCCGCCGATGCTGCCGCTGTGGCGCGGGGCGGTGAGGCCGCCCCGCGGGCCGTGGGCAGCCCGGCGGGgcgga > 1:138959/1‑133 (MQ=255) gagCGGCACGACCTTCGACAGCCTCAACCCGGCCCCCGCCCAGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag < 4:159781/135‑1 (MQ=255) gCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcagca < 7:69893/135‑1 (MQ=255) gCGGCAAGACCTGCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcagca < 2:47793/135‑1 (MQ=255) cGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGCCCCCAAGGCGTGGCgcagcct > 4:19534/1‑133 (MQ=255) gACCTTCGACGGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCCGACCGCCCAGCGTGCCTTCGACAGCCAGGCGTGGCGCAGCCTCACCg > 2:5162/1‑134 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCAACAGCCCGCCGCCGATGAAGCCGATGTGGAGCTTGCCGTTAAGGCCGCCCAGCGTGCCTTCGCCAGCAACGCGTGGCGCAGCATCACCg > 8:333608/1‑134 (MQ=255) tgcgccAGCCGCACCCCGGCCACCCCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGAGGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc < 6:254519/130‑1 (MQ=255) acacCCGCAACCCGCCCACCGCCACGGGCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgccc < 6:307511/135‑1 (MQ=255) gCCTCCCCCCGGCCCCCGCCCTGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 3:102293/135‑1 (MQ=255) gCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 4:224859/135‑1 (MQ=255) cTCACCCCGCCCCCCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGc < 6:115271/135‑1 (MQ=255) ccGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctg > 8:266592/1‑135 (MQ=255) ggCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 2:13683/135‑1 (MQ=255) aCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGCTGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTGCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGcgg > 8:48206/1‑134 (MQ=255) aCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCCCGGCCAGCGGCGGcgt > 8:150013/1‑135 (MQ=255) aCCGCCCAGGCCTGGGCGCAACAGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCCCTAACGCCCCCGGCAACCGGCTGcgg > 8:53383/1‑134 (MQ=255) cAGGCCTGGGCGCCACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCCGCATCACCGCTACCGCCCGCGGCCAGCTGCTGCgtcgtcgg > 8:60800/1‑133 (MQ=255) | AAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTG > KT2440/4135473‑4135740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |