Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,662,146 T→C 7.4% I124T (ATC→ACC)  nuoM → NADH‑quinone oxidoreductase subunit M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,662,1460TC 7.4% 166.2 / 4.5 54I124T (ATC→ACC) nuoMNADH‑quinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base T (21/29);  new base C (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01

ATCCACCTGGCCCTGGACGGCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTCACAGCTCGGCAGACGGCAAGAAGAC  >  KT2440/4662013‑4662278
                                                                                                                                     |                                                                                                                                    
aTCCACCTGGCCCTGGACGGCCTGTCGCTGCTGATGATCCTGCTAACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGAt                                                                                                                                      >  5:80113/1‑134 (MQ=255)
       tGGCCCTGGACGGCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTcggcg                                                                                                                              <  3:163232/135‑1 (MQ=255)
           ccTGGACGGCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGt                                                                                                                          <  5:308757/135‑1 (MQ=255)
           ccTGGACGGCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGt                                                                                                                          <  4:195114/135‑1 (MQ=255)
           ccTGGACGGCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGt                                                                                                                          <  2:64744/135‑1 (MQ=255)
                aCGGCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTc                                                                                                                      <  2:104635/134‑1 (MQ=255)
                 cGGCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGGGTGCTGTCGGTACGGTGTTCCTGGAAAGAGATCCAGCGCCCCGTCGGCTTCTTCCCCCGCAACCTGAGGTGGATCCTCCGCGGCGGGGGCgg                                                                                                                    >  7:143618/1‑135 (MQ=255)
                  ggCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACATGATGTGGATCCTCGGCGGCGTGGTCGgt                                                                                                                   >  8:336657/1‑135 (MQ=255)
                      tGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTTTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTTGATCCTCGGCGGCGTGGTCGgtgtgt                                                                                                               >  7:294027/1‑135 (MQ=255)
                      tGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGgtgtgt                                                                                                               >  2:161909/1‑135 (MQ=255)
                      tGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGgtgtgt                                                                                                               <  7:203397/135‑1 (MQ=255)
                      tGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGgtgtgt                                                                                                               <  3:289666/135‑1 (MQ=255)
                      tGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGgtgtgt                                                                                                               <  4:184312/135‑1 (MQ=255)
                      tGTCGCTGCGGATGATCCTGCTCACCGGCCTGCGCGGTGGGCTGTCGGTACTGTGTGCCTGGAAAGAGATCCAGCGCCACGGCGGCTGCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGgtgtgt                                                                                                               <  8:143618/135‑1 (MQ=255)
                       gTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGtt                                                                                                              >  6:113391/1‑135 (MQ=255)
                       gTCGCTGCTCATGATCCTGCTCACAGGCCTGCTAGGTGAGCTGTCGGTACAGTGTTCCTGGAAAGAGATCCAGCGCCCCGTCGGCTTCTTCCTCCTCAACCTGATGTGGATACTCGGCGGCCGGGTCtgtggggt                                                                                                              >  6:326650/1‑131 (MQ=255)
                            tgctgATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTgg                                                                                                         <  8:187134/135‑1 (MQ=255)
                                  tgatCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTgg                                                                                                   <  3:56855/135‑1 (MQ=255)
                                   gatCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGa                                                                                                  <  7:18963/135‑1 (MQ=255)
                                   gatCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGa                                                                                                  <  7:24032/135‑1 (MQ=255)
                                     tCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGAcc                                                                                                <  4:9187/135‑1 (MQ=255)
                                      ccTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATTTGGATCCTCGGCCGCGTGGTCGGTGTGTTCCTGGCCCTGGAcct                                                                                               >  6:107604/1‑135 (MQ=255)
                                        tGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGAcctg                                                                                              <  1:161909/134‑1 (MQ=255)
                                           tCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGAcctgttcc                                                                                          >  5:165120/1‑135 (MQ=255)
                                           tCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGAcctgttcc                                                                                          <  2:123034/135‑1 (MQ=255)
                                                  ccTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTgg                                                                                                         <  1:29378/113‑1 (MQ=255)
                                                  ccTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTgg                                                                                                         >  2:29378/1‑113 (MQ=255)
                                                     gCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGttcttctt                                                                                <  6:80113/135‑1 (MQ=255)
                                                         ggTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGttcttcttcttc                                                                            >  1:175310/1‑135 (MQ=255)
                                                              gCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTGGGTGGGTTACAGGCCCTGGGCCGGTTGCGGTTGGTCTTGTTATggg                                                                        >  5:192103/1‑134 (MQ=255)
                                                                     gTAGTGTGTTCCTGGATTGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGAGCTGTTCCTGTTCTTCTTCTTCTGGGATatgat                                                                 <  4:282521/134‑1 (MQ=255)
                                                                      tACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGc                                                               <  7:279333/135‑1 (MQ=255)
                                                                         tgtgTTCCTGGAAAGAGATACAGCGCCACGTCGTCTTCTTCCAACTCAACCTGATGTTGATCCTCGTCGGATTGGTCGGTGTGTTCCTGTCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGTAAATGATGCTg                                                             <  7:336657/134‑1 (MQ=255)
                                                                          gtgtTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGGGTGTTCCTGGCCCGGGACCTGGTCCTGTTCTTCTTGTTGGGGGAAAAGATGCTGGt                                                           >  2:111669/1‑135 (MQ=255)
                                                                            gtTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCGACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGc                                                         <  1:154880/135‑1 (MQ=255)
                                                                               ccTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTc                                                                                                                      >  4:46517/1‑71 (MQ=255)
                                                                               ccTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTc                                                                                                                      <  3:46517/71‑1 (MQ=255)
                                                                               ccTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGa                                                      >  4:48088/1‑135 (MQ=255)
                                                                               ccTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGa                                                      >  7:52793/1‑135 (MQ=255)
                                                                               ccTCGAAAGAGATACAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGCTCGGTGTGTTCCTGGCCCTGGACCTGTTCCAGTTCTTCTTCTTATTGGAAATGATGCAGGTGCCGa                                                      >  6:71955/1‑135 (MQ=255)
                                                                                        agaTCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTcc                                             >  3:328792/1‑135 (MQ=255)
                                                                                          aTCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTc                                           <  1:260912/135‑1 (MQ=255)
                                                                                             cAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGACCCTCGGCGGCGTGGTCGGTGTGTTCCTgg                                                                                                         >  5:37569/1‑70 (MQ=255)
                                                                                             cAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGACCCTCGGCGGCGTGGTCGGTGTGTTCCTgg                                                                                                         >  5:42123/1‑70 (MQ=255)
                                                                                             cAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGACCCTCGGCGGCGTGGTCGGTGTGTTCCTgg                                                                                                         <  6:42123/70‑1 (MQ=255)
                                                                                             cAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGACCCTCGGCGGCGTGGTCGGTGTGTTCCTgg                                                                                                         <  6:37569/70‑1 (MQ=255)
                                                                                                 gcCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCgcgc                                    >  3:168122/1‑135 (MQ=255)
                                                                                                               ttcCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTcaca                        <  4:328792/133‑1 (MQ=255)
                                                                                                                  cACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTATGGGAAATGATGCTGGTGCCGATGTTCTTCCGCATCGCGCTCTGGGGTAACAGcgcg                   >  6:116779/1‑135 (MQ=255)
                                                                                                                       cAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTGCTGGCCCTGGCCCAGTTCCGGTTGTTCTTCTTCTGGGAAATGATGGTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTCGCCGCACGGCAGa              >  5:284028/1‑135 (MQ=255)
                                                                                                                           cTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTCACAGCTCGGCAGACGGc          <  5:40523/135‑1 (MQ=255)
                                                                                                                            tGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTGTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTCACAGCTCGGCAGACGGCa         <  7:43410/135‑1 (MQ=255)
                                                                                                                            tGATGTGGATCCTCGGCGGCGTGGTCGGTGTGGTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTCACAGCTCGGCAGACGGCa         <  8:154117/135‑1 (MQ=255)
                                                                                                                             gATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTCACAGCTCGGCAGACgg           >  8:230214/1‑132 (MQ=255)
                                                                                                                                   gATCCTCGGCGGGGTGGTCGGTGTGTTCCTGGCCCGGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTCACAGCTCGGCAGACGGCAAGAAGAc  <  1:201955/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
ATCCACCTGGCCCTGGACGGCCTGTCGCTGCTGATGATCCTGCTCACCGGCCTGCTCGGTGTGCTGTCGGTACTGTGTTCCTGGAAAGAGATCCAGCGCCACGTCGGCTTCTTCCACCTCAACCTGATGTGGATCCTCGGCGGCGTGGTCGGTGTGTTCCTGGCCCTGGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTCTGGGGTCACAGCTCGGCAGACGGCAAGAAGAC  >  KT2440/4662013‑4662278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑