| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,874,567 | A→C | 34.1% | intergenic (+13/‑174) | CJ019_04568 → / → uacT_1 | hypothetical protein/Uric acid transporter UacT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,874,567 | 0 | A | C | 34.1% | 35.0 / 3.2 | 38 | intergenic (+13/‑174) | CJ019_04568/uacT_1 | hypothetical protein/Uric acid transporter UacT |
| Reads supporting (aligned to +/- strand): ref base A (8/16); new base C (12/2); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCC‑GCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGA > KT2440/4874436‑4874693 | gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCC‑GCTGGGCAACATCACCTAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCCACTGACCGCCCCTCGCCAGGc > 7:96638/1‑135 (MQ=255)gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCC‑GCTGGGCAACATCACCCAGAAGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCCAACGCGCCCCGGTCAACTCACAGCCCCTCGCCAGGc > 8:178625/1‑135 (MQ=255) ttCTGGCGCTTGCGGTTGGCAGCCAGATCATGCC‑GGTGGTCAACATCACCCATTTGACCACCGTAGAGCGCAAGGTGTTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc < 6:120116/135‑1 (MQ=255) ttCCGGCCCAAGCGGTTGACCCCCAGATCAGCCC‑GCGGGGCAACATCCCCCAGATCACCCCCTAAGCGCGCACGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc < 6:1165/135‑1 (MQ=255) ttCAGGCGAAAGGGGTTGCCAGCCAGATCAGGCC‑GCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc < 7:285040/135‑1 (MQ=255) gggggggccagccaTATCATCCC‑GCGGGGAAACACCCCCCAGATGCCCCCCGAAGCGCCCACGGGGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCaa < 3:31371/129‑1 (MQ=255) gCGGTTGCCCGCCATAGC‑TGCCGGGTGGGCAACATCACCCAGATGCCCACCGACGCGCGCAAGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCaa < 4:259641/135‑1 (MQ=255) cGGTTGCCAGCCAGATCATGCC‑GCTTGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCCAg > 1:281059/1‑135 (MQ=255) ccagccagATCATGCC‑GCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCCGCAACCCCCCCCCCCAGGGAATc > 5:74860/1‑135 (MQ=255) gagccagTTCATGCC‑TGGGGGAAAATTACCCCAGATGACCACCTAAGTGCGCAAGATCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCa < 4:252441/134‑1 (MQ=255) cagcctgAACATGCC‑GGGGGGCAACATCACCCATATGACCACCGAAGAGCGAAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCa < 6:71258/135‑1 (MQ=255) cagATCATGCC‑GCTGGGCAACATCACCCAGATGACCACCGACGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCtt < 8:89168/135‑1 (MQ=255) agATCATGCC‑GCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGGTCGCCAAAGGCGCCCAGGGCAACTGACCGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCCCCTTc > 1:242288/1‑135 (MQ=255) gATCATGCC‑GCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCACCCCGCCCCCCCAGGGAATCCCCGTcc > 3:117480/1‑135 (MQ=255) gATCATGCC‑GCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCACGGCAACTCACAGCACCTCGCCCGGCAACCCGCCCCCCAACGCAAACACCATcc > 3:149214/1‑135 (MQ=255) aTGCC‑GCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGCATCACCTTCCCCTg > 8:256431/1‑135 (MQ=255) gaaaCATCACCCAGAGCCCCACCTACGCGCCCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCgg < 6:82502/132‑1 (MQ=255) gCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCCCCTTCCCCTGCAGGCGCggg > 3:209018/1‑135 (MQ=255) aaCATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCt > 8:110445/1‑135 (MQ=255) cACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGCCAGCACCTCGCCCGGCCACCCGCCCCCCCAGGGAATCCCCGTCCCCGGCAGGCGCGGGCTTGccc > 3:326528/1‑135 (MQ=255) cACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCCCCTCGCCCGGCAACCCGCCCCCCCAGGGCATCACCTTCCCCGGCAGGAGCGGGCTTGccc > 5:79584/1‑135 (MQ=255) cccAGATCCCCCCCGAAGCGCGCAAGCGGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCcgc < 4:99813/135‑1 (MQ=255) ccAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCCACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCCCCTTCCCCTGCAGGAGCGGGCTTGCCcgc > 8:205113/1‑134 (MQ=255) ccccccGACGAGCGCAAGCGCGGCGGCGACTGCAGCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCCCCAGCCAACCCGCCCCCCAAGGCAATCACCTTCCCCTGCAGGAGCGGGCTGGCCCGCGAAGAGGc < 2:71546/131‑1 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCCAAGGCGCCCAGGGCAACTGACAGCACCTCGCCCGGCAACCCCCCCCCCCAGGGCATCACCGTCCCCCGCCGGAGCGGGCGTGCCCGCGACGAGGc > 1:143402/1‑135 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTAGCCCGGCAACCCCCCCCCCAACGGAATCACCTTCCCCTGCAGGAGCGGGCGTGCCCGCGCAGCGGc > 7:76888/1‑135 (MQ=255) cGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCCAAGGCGCCCAGGTCAACTGACAGCACCACGCCCCGCAACCCCCCCCCCCACGGCATCCCCGTCCCCAGCAGGCGCGGGCGTGGCCGCGCACAGGCCGCTa > 6:296003/1‑135 (MQ=255) gCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGCAGAGGCCGCTAGCCCCAGCCt > 7:22811/1‑133 (MQ=255) cTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCCACCCGCCCCCCCAGGGCATCCACTTCCCCGGCAGGAGCGGGCGTGCCCGCGCAGAGGCCGCTAGCCCCAGCCGCaa > 8:15036/1‑135 (MQ=255) accGCCAACGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCCCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg < 4:66836/133‑1 (MQ=255) ccAATGGCGCCACGTTAAACTGACACCACCTCCCCCGCCAACCCCCCCCCCACGTTAAGCCCCTACCACTGCGGCTGGGGGCTTGCCCTCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac < 2:270686/135‑1 (MQ=255) ccAAAGGCGCCCAGGTCAACTCACCGCACCGCGCCAGGAAACCCCCCCCCCAAGGGAATCACATTCCCCGGCTGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac < 8:67051/135‑1 (MQ=255) gcAACCGACAGCACCGCGCCAGCCACCCCCCCCCCCACGGTAAGCAGCTTCCCCGCCAGGAGCGGGCTGGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 5:223036/134‑1 (MQ=255) aaCTCACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTGCCCCGGCCGGAGCGGGCTGGCCGGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgcgc < 4:149214/135‑1 (MQ=255) aCTGACAGCACCTCCCCCGGCAACCCGCCCCCCACGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgcgc < 4:117480/134‑1 (MQ=255) tGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTACCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGACCCAGCCTCAAGCTCCATGTGTTACGCTACAAGCAAGCATCGAgcgcgcc > 7:18160/1‑134 (MQ=255) aCAGCACCCCGCCAGGCCACCCGCCCCCCAAGGGAATCACCTACCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAgg < 6:258030/135‑1 (MQ=255) aCCTCGCCAGGCACCCCGCCCCCCAAGGGAATCCCCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCCAGCTCCATGTGGTACGCAACAAGCACGCATCGAGCGCGCAGGCATGa > 3:8193/1‑135 (MQ=255) | GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCC‑GCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGA > KT2440/4874436‑4874693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |