Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,441,450 G→C 5.7% E377Q (GAG→CAG)  hemL → Glutamate‑1‑semialdehyde 2,1‑aminomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,441,4500GC 5.7% 219.7 / 4.8 70E377Q (GAG→CAG) hemLGlutamate‑1‑semialdehyde 2,1‑aminomutase
Reads supporting (aligned to +/- strand):  ref base G (31/35);  new base C (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

GCCTGCAGCAACGCGCCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATGCGGCTGAGAA  >  KT2440/5441322‑5441583
                                                                                                                                |                                                                                                                                     
gCCTGCAGCAACGCGCCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCt                                                                                                                                 >  8:177784/1‑135 (MQ=255)
    gcagcaACGCGCCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCaa                                                                                                                             >  8:60041/1‑135 (MQ=255)
      agcaACGCGCCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGCCGATATCCTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAgc                                                                                                                           >  1:224352/1‑135 (MQ=255)
      agcaACGCGCCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCaa                                                                                                                             <  6:37517/133‑1 (MQ=255)
      agcaACGCGCCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAgc                                                                                                                           >  7:165053/1‑135 (MQ=255)
              gcCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGcttctt                                                                                                                    >  1:197695/1‑134 (MQ=255)
              gcCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGcttctt                                                                                                                    >  5:310484/1‑134 (MQ=255)
               ccGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGAGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTcc                                                                                                                  <  1:270501/135‑1 (MQ=255)
                cGATGCCGCTTGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGCCGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCa                                                                                                                 >  6:145247/1‑135 (MQ=255)
                cGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCa                                                                                                                 >  2:312864/1‑135 (MQ=255)
                cGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATAGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCa                                                                                                                 >  5:64193/1‑135 (MQ=255)
                  aTGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCAcc                                                                                                               >  7:144336/1‑135 (MQ=255)
                       gCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATg                                                                                                          <  8:144336/135‑1 (MQ=255)
                           gCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCg                                                                                                      <  7:148617/135‑1 (MQ=255)
                           gCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCg                                                                                                      <  3:43676/135‑1 (MQ=255)
                           gCGTGCCACTCGTCACCACCCAGGCCGGGGACATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCg                                                                                                      <  7:121661/135‑1 (MQ=255)
                            cGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCg                                                                                                      <  6:69778/134‑1 (MQ=255)
                                 cATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTg                                                                                                >  3:204998/1‑135 (MQ=255)
                                    tCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCg                                                                                             <  5:266639/135‑1 (MQ=255)
                                      gTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCgt                                                                                            >  7:326664/1‑134 (MQ=255)
                                                aGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCAc                                                                                 >  6:268673/1‑135 (MQ=255)
                                                 ggCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCAcc                                                                                <  2:215276/135‑1 (MQ=255)
                                                 ggCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCAc                                                                                 >  6:338695/1‑134 (MQ=255)
                                                  gCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCg                                                                               <  3:39043/135‑1 (MQ=255)
                                                  gCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCg                                                                               <  8:214588/135‑1 (MQ=255)
                                                   ccGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGa                                                                              <  8:239665/135‑1 (MQ=255)
                                                   ccGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGa                                                                              >  4:331766/1‑135 (MQ=255)
                                                     ggTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCCGGCACCGAgc                                                                            >  6:300396/1‑135 (MQ=255)
                                                        gCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCa                                                                         >  1:285683/1‑135 (MQ=255)
                                                         ccATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCCAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAt                                                                        >  8:145947/1‑135 (MQ=255)
                                                         ccATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCCAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAt                                                                        >  8:141461/1‑135 (MQ=255)
                                                          taTGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAtt                                                                       >  3:57883/2‑135 (MQ=255)
                                                          cATGTTCTGCCTGTACTTCAGCGGCGCCGACGATATCGTCACATTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAtt                                                                       >  4:276636/1‑135 (MQ=255)
                                                          cATGTTCGGCCTGTACTTCAGCTGCGCCGACGATATCGACACCTTCGAAGACGTGATGGCCAGCGATGCCGATCGCTTCAAGAGCTTCTTCCACATGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAta                                                                       >  3:129464/1‑134 (MQ=255)
                                                          cATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAtt                                                                       >  2:259423/1‑135 (MQ=255)
                                                          cATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAtt                                                                       >  1:327313/1‑135 (MQ=255)
                                                          cATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAtt                                                                       >  7:44529/1‑135 (MQ=255)
                                                          cATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCAtt                                                                       >  7:299888/1‑135 (MQ=255)
                                                            tGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCg                                                                     <  5:20719/135‑1 (MQ=255)
                                                              ttCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCg                                                                     <  2:304083/133‑1 (MQ=255)
                                                                   ccTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCggg                                                              <  5:234876/135‑1 (MQ=255)
                                                                      gTACTTCAGCGGCGCCGACGATTTCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCtt                                                           <  4:1392/135‑1 (MQ=255)
                                                                      gTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGCCCAGCGATCCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGAGCATTCGAAGCGCGCtt                                                           >  7:130198/1‑135 (MQ=255)
                                                                          ttCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCAcc                                                       >  8:40769/1‑135 (MQ=255)
                                                                          ttCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGGGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGCGCGCATTCGAAGCGGGCTTCAcc                                                       >  3:304852/1‑135 (MQ=255)
                                                                             aGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCt                                                      <  2:327313/133‑1 (MQ=255)
                                                                                ggcgCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCCCCGACCGCATTCGCAGCGGGCTTCAcctccctc                                                 >  5:3645/1‑135 (MQ=255)
                                                                                  cgcCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGc                                               <  3:289289/135‑1 (MQ=255)
                                                                                    ccGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGAt                                                                                                           <  6:245334/73‑1 (MQ=255)
                                                                                    ccGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGAt                                                                                                           >  5:245334/1‑73 (MQ=255)
                                                                                       acgaTATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATg                                          >  8:7485/1‑135 (MQ=255)
                                                                                          atatCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCg                                       <  7:140013/135‑1 (MQ=255)
                                                                                          atatCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCg                                       <  6:62867/135‑1 (MQ=255)
                                                                                          atatCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCg                                       <  4:84040/135‑1 (MQ=255)
                                                                                                tCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCGCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAg                                  <  6:280363/134‑1 (MQ=255)
                                                                                                   ccTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAg                               <  4:204998/134‑1 (MQ=255)
                                                                                                        gAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGc                           <  2:224352/133‑1 (MQ=255)
                                                                                                        gAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGc                           <  4:242294/133‑1 (MQ=255)
                                                                                                        gAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCa                          <  2:197695/134‑1 (MQ=255)
                                                                                                        gAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCa                          <  6:310484/134‑1 (MQ=255)
                                                                                                                gATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCt                 <  1:259423/135‑1 (MQ=255)
                                                                                                                  tGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTgg               <  8:326664/135‑1 (MQ=255)
                                                                                                                     ccAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATg            <  5:338695/135‑1 (MQ=255)
                                                                                                                     ccAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATg            <  8:271441/135‑1 (MQ=255)
                                                                                                                     acAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATg            <  8:202187/134‑1 (MQ=255)
                                                                                                                         cGATGCCGAGCGCTTCGAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCCGTCGAAGCGGGCTTCACCTCCATCGCCGATGGCGACAAGGAGCTGCGGATCACCCTCGATGCGGc        >  1:193750/1‑135 (MQ=255)
                                                                                                                            tGCCCAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATGCGGCTga     <  7:145947/135‑1 (MQ=255)
                                                                                                                            tGCCCAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATGCGGCTga     <  7:141461/135‑1 (MQ=255)
                                                                                                                               cGAGCGCTTCAAGCGCTTCTTCCACCTGCTGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATGCGGCTGAGaa  <  6:10888/135‑1 (MQ=255)
                                                                                                                               cGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATGCGGCTGAGaa  <  3:153328/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                                     
GCCTGCAGCAACGCGCCGATGCCGCTGGCGTGCCATTCGTCACCACCCAGGCCGGTGCCATGTTCGGCCTGTACTTCAGCGGCGCCGACGATATCGTCACCTTCGAAGACGTGATGGCCAGCGATGCCGAGCGCTTCAAGCGCTTCTTCCACCTGATGCTCGACGGTGGCGTGTACCTGGCACCGAGCGCATTCGAAGCGGGCTTCACCTCCATCGCCCATGGCGACAAGGAGCTGCAGATCACCCTGGATGCGGCTGAGAA  >  KT2440/5441322‑5441583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑