Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,585,616 T→A 6.2% D28V (GAC→GTC)  nudF ← ADP‑ribose pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,585,6160TA 6.2% 200.6 / 3.5 65D28V (GAC→GTC) nudFADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base A (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

TCGATCAGCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTCGCGCCATCCTACTC  >  KT2440/5585484‑5585742
                                                                                                                                    |                                                                                                                              
tggATCAGCACCTCTTCTTCGGGCGGGGGGTCGTTCGGCAGCACTCATACCGCATCGTGGGGCACAAAAAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGTCGCAGGCGTAACTTGTc                                                                                                                               <  1:201851/132‑1 (MQ=255)
tCGATCTGCACCACTTCATCGCGCGCTGGGCCGGACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGGCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCg                                                                                                                              <  7:3454/135‑1 (MQ=255)
gcGAGCAGCACCCCTTCATCGCGCGCGGGGGCGTCCGGCAGCACCCATCCCGCAGCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCg                                                                                                                              <  5:36275/134‑1 (MQ=255)
  gATCAGCACCCCTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGCCGCACCAACAGTTCGCGACTGATCTCGAGCCCCATGCCCCCGGCAAAAAGCTCGTGGCGCAGCCGTATCTTGTCGAg                                                                                                                            >  2:70700/1‑135 (MQ=255)
  gATCAGCACCACTTCATCGCGCGCTGTGTCGTACGGCAGCACGCATCCCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAg                                                                                                                            >  8:100952/1‑135 (MQ=255)
  gATCAGCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAg                                                                                                                            >  5:264367/1‑135 (MQ=255)
  gATCAGCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAg                                                                                                                            >  3:181536/1‑135 (MQ=255)
  gATCAGCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAg                                                                                                                            >  2:304402/1‑135 (MQ=255)
   aTCAGCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCCTGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTACCTTGTCGAGc                                                                                                                           >  8:250772/1‑135 (MQ=255)
      aGCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTg                                                                                                                        <  2:111084/135‑1 (MQ=255)
       gCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCTCGCCCCATGCCCACGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTg                                                                                                                        >  1:202795/1‑134 (MQ=255)
       gCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGt                                                                                                                       <  5:154281/135‑1 (MQ=255)
          ccacTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGt                                                                                                                       <  6:337013/132‑1 (MQ=255)
          ccacTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGt                                                                                                                       >  5:337013/1‑132 (MQ=255)
          ccacTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTa                                                                                                                      >  3:162049/1‑133 (MQ=255)
          caacTTCATCGCGCGCTGGGTCGTACGGCATCACGCATACAGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCAGGCAAATAGCTCGAGGCGCAGGCGTAACTTGTCGAGCTTGtt                                                                                                                      >  2:222377/3‑132 (MQ=255)
                cATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCCTCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCt                                                                                                              <  7:236446/135‑1 (MQ=255)
                cATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCt                                                                                                              >  4:283332/1‑135 (MQ=255)
                 aacgcgcgCTGGGTAGGAAGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCt                                                                                                              <  4:191993/132‑1 (MQ=255)
                 aTCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCt                                                                                                              <  3:283332/134‑1 (MQ=255)
                   cgcgcgCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTg                                                                                                             >  4:271043/1‑133 (MQ=255)
                               cGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGccc                                                                                               >  6:187798/1‑135 (MQ=255)
                                  aCGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCt                                                                                            <  8:243860/135‑1 (MQ=255)
                                   cGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTc                                                                                           <  5:335899/135‑1 (MQ=255)
                                         caCGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCgatgat                                                                                     >  8:294958/1‑135 (MQ=255)
                                           cGCATTCCGCATCTTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCGCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGAttt                                                                                   <  3:219312/135‑1 (MQ=255)
                                           cGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGAttt                                                                                   <  3:271043/135‑1 (MQ=255)
                                              aTACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTc                                                                                  >  4:14882/1‑133 (MQ=255)
                                                 ccGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCAGCTCGATGATTTCGAAGc                                                                             >  6:1851/1‑135 (MQ=255)
                                                  cGCATCGGGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGc                                                                             <  7:107072/134‑1 (MQ=255)
                                                   gCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGccc                                                                           >  1:210202/1‑135 (MQ=255)
                                                      tCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTg                                                                        >  4:107569/1‑135 (MQ=255)
                                                       cGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTgg                                                                       >  3:269898/1‑135 (MQ=255)
                                                       cGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGACGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGAttt                                                                                   <  6:142105/123‑1 (MQ=255)
                                                       cGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGACGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGAttt                                                                                   <  6:136410/123‑1 (MQ=255)
                                                       cGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGACGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGAttt                                                                                   >  5:142105/1‑123 (MQ=255)
                                                       cGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGACGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGAttt                                                                                   >  5:136410/1‑123 (MQ=255)
                                                        gTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTgg                                                                       <  5:187798/134‑1 (MQ=255)
                                                         tGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGc                                                                     >  2:259020/1‑135 (MQ=255)
                                                               acaAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCg                                                               >  5:283640/1‑135 (MQ=255)
                                                               acaAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCg                                                               >  4:19860/1‑135 (MQ=255)
                                                               acaAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCg                                                               >  8:122506/1‑135 (MQ=255)
                                                                 aaaCAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGt                                                              >  5:236278/1‑134 (MQ=255)
                                                                          tcgACTGATCTCGAGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTc                                                    <  5:32860/134‑1 (MQ=255)
                                                                            gACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCACCGCGTCTg                                                  >  4:261646/1‑135 (MQ=255)
                                                                                  tctcGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATg                                             <  2:297891/134‑1 (MQ=255)
                                                                                       cgcCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGa                                           <  8:242853/131‑1 (MQ=255)
                                                                                         ccccATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATcc                                      >  1:129769/1‑134 (MQ=255)
                                                                                          cccATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTc                                    <  6:236278/135‑1 (MQ=255)
                                                                                            cATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCa                                   >  7:179720/1‑134 (MQ=255)
                                                                                             aTGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCAtt                                 <  2:210202/135‑1 (MQ=255)
                                                                                              tGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCGTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTa                                >  2:226274/1‑135 (MQ=255)
                                                                                                cccccGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCGTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTAca                              >  7:225313/1‑134 (MQ=255)
                                                                                                       cAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCCCAGCGTTCCACGCGTCTGACATGGAAATCCTCATTACCGCGAATa                       >  5:33382/1‑135 (MQ=255)
                                                                                                              cTCGTGGCGGAGGCGTAACTTGTCGAGCGTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGAGATGGAAATCCTCATTACCTCTAATACTGCTTc                <  7:253330/135‑1 (MQ=255)
                                                                                                              cTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTc                <  7:200096/135‑1 (MQ=255)
                                                                                                              cTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTc                <  7:294958/135‑1 (MQ=255)
                                                                                                              cTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTc                <  2:199191/135‑1 (MQ=255)
                                                                                                              cTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTc                <  8:179720/135‑1 (MQ=255)
                                                                                                              cTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTc                <  1:304402/135‑1 (MQ=255)
                                                                                                              cTCGTGGCGCAGGAGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTc                <  1:222377/135‑1 (MQ=255)
                                                                                                               tCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTc                <  7:100952/134‑1 (MQ=255)
                                                                                                                 gTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGa                          >  2:13725/1‑122 (MQ=255)
                                                                                                                 gTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGa                          <  1:13725/122‑1 (MQ=255)
                                                                                                                            gTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTCGCGCCATCCTACTc  >  3:179134/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                              
TCGATCAGCACCACTTCATCGCGCGCTGGGTCGTACGGCAGCACGCATACCGCATCGTGGCGCACAAACAGTTCGCGACTGATCTCGCGCCCCATGCCCCCGGCAAATAGCTCGTGGCGCAGGCGTAACTTGTCGAGCTTGTAAAAGCCCTGGAAGCAATTGGCCCGCTCGATGATTTCGAAGCCCTTGGGCACAGCGTTCAACGCGTCTGACATGGAAATCCTCATTACCTCGAATACTGCTTCGCGCCATCCTACTC  >  KT2440/5585484‑5585742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑