| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,689,824 | T→G | 59.6% | R238R (CGT→CGG) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,689,824 | 0 | T | G | 59.6% | 0.7 / 4.8 | 27 | R238R (CGT→CGG) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (4/7); new base G (2/14); total (6/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCG > KT2440/5689698‑5689950 | cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCCCGTCAGCCCGCTCCCCACCGCCCGGGGcgcgcg > 6:171568/1‑135 (MQ=255) aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGGGCGCGGAAGCCACAACACCAGCGCCCCGGGcggccggcgggta > 1:143302/1‑130 (MQ=255) gttCCTCGGCCGGCCGGGCAGCCCGGAGCGCCCCCAAACGGGGGCCGGGGGGCGCAACGGGCGGCTGGGGCAACCGCGCGGCGTGCGGGCGCACTTCCGCCTCACCACCCGCGCCGGGGGCGCGCGGCTGATCgc < 4:155236/134‑1 (MQ=255) ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGGGCCCTTCAGCCCCCTCACCACCGCCCCTGGCGCGCGGCGGATCgcccc > 7:257325/1‑134 (MQ=255) tCGGCCTGCCGGGAATCCCGCATAGCGCCGAAACGGTGGCCCTGGCGCGAAACCTGCTGCGGTTGTAACCGAGGGGGGTGCGTGCGCACTTCACCCCTACCCCCCGCGCCGGGGGCGCGCGGCTGATCgcccagg < 6:123148/135‑1 (MQ=255) tCGGCCGGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCGGGGCGCGCAACCTGCTGCTGGTGGAACAGCGCGGCGGGCGTGCGCACTGCAGCCAGAGCACCCGCGCCCGTGGCGCGCGGCTGATCgcccagg < 8:257325/135‑1 (MQ=255) gtagcgcgcGGAAACCGTGGCCCGGGCGCGCAACCTGCTGCGGTTGCAACAGCGCGGCGGGCGGGCTCACTTCAGCCATACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc < 2:250653/130‑1 (MQ=255) gccctaaaCCGGGGCCCTGGCGGGAAACTTGTGGGGGTTGTAACTGCGGGGGGTGCGGGCGCATTGCCGCCATATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 8:293965/130‑1 (MQ=255) ccccGAAACCGTGGCCCGGGCGCGAAACGTGCGGCGGGGGTAACAGCGCGGGGGGCGTGCGCACTACAGCCACACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 2:51756/133‑1 (MQ=255) aCCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTAATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 3:288712/133‑1 (MQ=255) ggggCCCGGGCGCGCAACCTGCTGCGGGTGGAACAGAGGGGCGTGCGGGCGCACTCCCGCAAGAGCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 3:157743/133‑1 (MQ=255) ggggCCCGGGCGCGCAACCTGCGGCGGGGGCACCCGCGGGGCGGGCGGGCGCACTCCCCCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 1:244788/133‑1 (MQ=255) ggggCCCGGGCGCGCAACCGGCTGGTGGTGCAACGGCGGGGCGGGCGTGCGCAGTACCGCCACACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 2:143302/133‑1 (MQ=255) gTGGCCGGGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 3:118119/135‑1 (MQ=255) gggAACAGCGGGGCGGGCGGGCGCACTTCCGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACg < 8:25082/134‑1 (MQ=255) gAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATAACCCGCCCCCGTGGCGCGCGGCTGATCGCCCCGGCCCCGGCCGTGGGCCGGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCGCACGGACgcg > 6:169056/1‑133 (MQ=255) cgcggggGGCGGGCGCACTCCCCCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg < 2:277482/134‑1 (MQ=255) ggcgtgccCACTTCCGCCATTACCCCTGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 5:74575/134‑1 (MQ=255) ggcgggcgCCCTCCAGCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 2:283392/134‑1 (MQ=255) cgggcgcACTTCCGCCCGCTCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc < 7:150747/132‑1 (MQ=255) gggcAGTTCCGCCACATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 4:332407/133‑1 (MQ=255) cccACTTCAGCCATCCCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCa < 1:305167/133‑1 (MQ=255) gaaGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGt < 8:285834/133‑1 (MQ=255) gcccccgcgcCCGGGGCGCGCGGCGGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGc < 1:213437/129‑1 (MQ=255) cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTACCCGCCGCTGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTCCCCCGTGCa > 2:141010/1‑135 (MQ=255) cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCCCCGATCTAGCGCTGTACCAGCTGATCCTCACGGACGACTCGCTGCGCGAGTGCTCCCGCCTGTCCCACGTGCa > 6:248962/1‑135 (MQ=255) ggcgcCCGGGGCGCGCGGCAGATCGCCCAGGCCCAGGCCGTGGGCCTGCCGGGCACCGCCGACCTAGCGCTGTCCCAGCTGATCCTCCCGGACGAGGCGCTGCGCGAGCTCTCCAGCCTGTACCACGTGCAgccg < 3:304008/134‑1 (MQ=255) | CATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCG > KT2440/5689698‑5689950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |