Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,757,828 G→A 13.3% intergenic (+52/‑27) envC → / → CJ019_05402 Murein hydrolase activator EnvC/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,757,8280GA13.3% 68.6 / 5.2 30intergenic (+52/‑27)envC/CJ019_05402Murein hydrolase activator EnvC/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base A (2/2);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01

CAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCGGCCAAGGCCGCTGCAGTGCCAGCC  >  KT2440/5757699‑5757962
                                                                                                                                 |                                                                                                                                      
cAGGAGGCGGCTGGGTTGTATTTCGCTACCCGCCAGCCGGGCCGGCCTACCGACCGTTCGCTGGGGGGCGGGGCCTAGGAAAATTTATAGGGCGAAGCGTGTGGGCGGCGCCGATGCTCCCCAGGGAGCGCTTAc                                                                                                                                   <  7:226144/135‑1 (MQ=255)
 aGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCTCCGATGCTCCCCAGGGAGCACTTACa                                                                                                                                  >  7:223933/1‑135 (MQ=255)
 aGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCACTTACa                                                                                                                                  >  7:218722/1‑135 (MQ=255)
           aGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCCGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACCGGCTCAGGAg                                                                                                                        >  6:200301/1‑135 (MQ=255)
                   tttgcGCTAGCCGCCTCCTGGGCGGGCCTACCGACCCTGCGCTGGGGTGCGGGGGCTAGTAAAATTTCTCCGGCGCTGCCTGAGTGCGGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAgttcgttc                                                                                                                 <  2:87198/130‑1 (MQ=255)
                   aTTTCGCTACCCGCCAGCCGGGCCGGCCTACCGACCCTTCGCAGGGGGGCCGGGGCTAGTAAAATTTCTACGGCGCAGCCTGAGGGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGttcgttcg                                                                                                                <  3:35695/135‑1 (MQ=255)
                     ttCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGAc                                                                                                              >  7:280416/1‑135 (MQ=255)
                     ttCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCACGGAGCGCTTACAGGATCAGGAGTTCGTTCGAc                                                                                                              >  3:272884/1‑135 (MQ=255)
                          tATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACAtgct                                                                                                         >  4:324505/1‑135 (MQ=255)
                          tATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACAtgct                                                                                                         >  2:335151/1‑135 (MQ=255)
                                         ccggccTACCTCCCCTTCGCAGTGGGGCCGGGGCTAGACAAATTTCTACGGCGCAGCGTGAGTGCGGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGtct                                                                                          <  4:329131/135‑1 (MQ=255)
                                           ggccTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGGCTCa                                                                                        >  5:269905/1‑135 (MQ=255)
                                               tACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCa                                                                                    >  1:121799/1‑135 (MQ=255)
                                                   gACCCTTCGCAGTGGGGCCGGGGCTAGCAAAATTTCTACGGCGCAGCCTGAGGGCGGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTg                                                                                <  2:19592/135‑1 (MQ=255)
                                                                          cTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGt                                                         >  1:285357/1‑135 (MQ=255)
                                                                            aggaaaaTTTCTCCGGCCCAGCCGGTGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGAGCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCg                                                       <  4:272884/131‑1 (MQ=255)
                                                                              tCAAATTTCTCCGGCGCAGCCTGAGTGCGGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCg                                                       <  4:249847/133‑1 (MQ=255)
                                                                              tCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCg                                                       <  8:280416/133‑1 (MQ=255)
                                                                               cAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGgcg                                                    >  5:104284/1‑135 (MQ=255)
                                                                                    ttCTCCGGCGCAGCCGGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCTCCCTGGCGGTCGGCGCGccc                                               <  4:43374/135‑1 (MQ=255)
                                                                                                        gctgcGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCCCCGCCGCAGAGcc                           >  3:46691/1‑135 (MQ=255)
                                                                                                         ctgcGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCg                          >  4:320473/1‑135 (MQ=255)
                                                                                                         ctgcGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCGCACCCAGCTGGCCCTGTCCCTCGCCCTGGCGGTCGGCGCGCCCCTGGCCCCCGCCGCAGCGCCg                          >  4:263414/1‑135 (MQ=255)
                                                                                                         cggggcCGATGCTCCCCAGGGAGCGCTTCCAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTTTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCg                          <  6:301094/131‑1 (MQ=255)
                                                                                                            cgcCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCCCCGCCGCAGAGCCGGc                        >  7:42679/1‑134 (MQ=255)
                                                                                                              ccGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCg                          >  2:190686/1‑130 (MQ=255)
                                                                                                              ccGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCg                          <  1:190686/130‑1 (MQ=255)
                                                                                                              ccGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTACGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGACCTGGCGGTCGGCCCGCCCCTGGCCACCGCCGCAGAGCCGGCCaa                     >  7:59772/1‑135 (MQ=255)
                                                                                                                          aGGGAGCACTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCGGCCAAGGCCGCTGCAGt         <  8:218722/135‑1 (MQ=255)
                                                                                                                          aGGGAGCACTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCGGCCAAGGCCGCTGCAGt         <  8:223933/135‑1 (MQ=255)
                                                                                                                                 gcTTACAGGATCAGGAGTTGGTGCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCGTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCGGCCAAGGCCGCTGCAGTgccagcc  <  6:222322/135‑1 (MQ=255)
                                                                                                                                 gcTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCGGCCAAGGCCGCTGCAGTgccagcc  <  5:25528/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                      
CAGGACGCGGCAGGCTTGTATTTCGCTATCCGCCAGCAGGGCCGGCCTACCGACCCTTCGCAGTGGTGCCGGGGCTAGTCAAATTTCTACGGCGCAGCCTGAGTGCTGCGCCGATGCTCCCCAGGGAGCGCTTACAGGATCAGGAGTTCGTTCGACATGCTGCACTCGCCTCGTCTCACCCAGCTGGCCCTGTCCATCGCCCTGGCGGTCGGCGCGCCCCTGGCCACCGCCGCAGAGCCGGCCAAGGCCGCTGCAGTGCCAGCC  >  KT2440/5757699‑5757962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑