Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,757,234 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,757,2340A.90.9% 127.2 / 2.9 33coding (776/834 nt)CJ019_05400hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base . (28/2);  minor base T (0/2);  total (28/5)
Fisher's exact test for biased strand distribution p-value = 1.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTT  >  KT2440/5757107‑5757351
                                                                                                                               |                                                                                                                     
cAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaacgc                                                                                                                >  6:5204/1‑132 (MQ=255)
         gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGCCCCGTGAAGCTGAAGAGGCTCGCAAGAAGCCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCcagca                                                                                                       >  1:42157/1‑134 (MQ=255)
           aCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGCAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCACGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCCCcagcag                                                                                                      >  5:274665/1‑133 (MQ=255)
                       cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGgccgc                                                                                        >  4:162338/1‑135 (MQ=255)
                       cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGgccgc                                                                                        >  2:269384/1‑135 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAACCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGACGCGCTGGccgccgccg                                                                                   >  5:4462/1‑135 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                   >  4:3026/1‑135 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                   >  2:15698/1‑135 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                   >  2:274340/1‑135 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                   >  3:225703/1‑135 (MQ=255)
                                      cTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCAGCCcgcgcc                                                                        >  7:263277/1‑134 (MQ=255)
                                           aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGc                                                                   >  7:52412/1‑136 (MQ=255)
                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGGGa                                                             >  4:158448/1‑135 (MQ=255)
                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGa                                                             >  1:216553/1‑135 (MQ=255)
                                                  cGAGGAAACCCTCGCCCGCCAGCCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                             >  3:183192/1‑135 (MQ=255)
                                                                          cggggAAGCTTAAGAGGCACGAAAGAAGGCCCGGCGGGCGCAGCTGTAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                    <  7:48343/132‑1 (MQ=255)
                                                                           ggTGACGCTGATGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                    <  4:139896/134‑1 (MQ=255)
                                                                            gTGACGCTGAAGCGGCACGCAAGACGGCCCTGCTGGCGCAGCCGGACGCGTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGa                                   <  2:30400/135‑1 (MQ=255)
                                                                                aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAACACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGCGCCAGTGGACCCGCCGaaaaa                               >  2:59042/1‑135 (MQ=255)
                                                                                aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                               >  1:72510/1‑135 (MQ=255)
                                                                                aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                               >  1:55512/1‑135 (MQ=255)
                                                                                aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                              >  8:197103/1‑136 (MQ=255)
                                                                                     aaGAGGCACGCAAGAAGGCCCTGCTGGCGCATCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGACTGCAGAGCCAGTGGAACCGCCGAAAAAGGccc                          >  2:199936/1‑135 (MQ=255)
                                                                                            acccacgaagGCCGTGCGGGCGCAGCAGGACGCGTAAAAACGCCGCCAGCAGGACGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                   <  5:238243/129‑1 (MQ=255)
                                                                                             cGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCAccc                 >  7:263557/1‑136 (MQ=255)
                                                                                               cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAACGGCCCGTACCACCCt                >  1:45021/1‑135 (MQ=255)
                                                                                               cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCAcccg                >  2:105989/1‑134 (MQ=255)
                                                                                               cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc               >  8:139342/1‑136 (MQ=255)
                                                                                                  gaagGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGCGCCAGTGGAACCGCCGAAAAAGGGCCGTACCCCCCTCggg            >  7:239477/1‑136 (MQ=255)
                                                                                                  gaagGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCgg             >  3:182383/1‑135 (MQ=255)
                                                                                                   gagGCCCTGGTGGGGCACCAGTAAGCGAAAAAACGCCGCCAGCTGGAAGCGCGGGCGGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc           <  8:263277/135‑1 (MQ=255)
                                                                                                           gctgGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGt    >  1:159929/1‑135 (MQ=255)
                                                                                                           gctgGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGGGCGCCTGCCGCGCCAGGGGCACCGCCGAAAAAGGCCCGTTCCACCCACGGGCCGATggg    >  2:141165/1‑134 (MQ=255)
                                                                                                             tgGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt  >  1:68830/1‑135 (MQ=255)
                                                                                                                               |                                                                                                                     
CAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTT  >  KT2440/5757107‑5757351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: