| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 407,069 | A→G | 15.4% | intergenic (+39/‑164) | glnE → / → rfaF | Bifunctional glutamine synthetase adenylyltransferase/adenylyl‑removing enzyme/ADP‑heptose‑‑LPS heptosyltransferase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 407,069 | 0 | A | G | 15.4% | 60.1 / 5.6 | 26 | intergenic (+39/‑164) | glnE/rfaF | Bifunctional glutamine synthetase adenylyltransferase/adenylyl‑removing enzyme/ADP‑heptose‑‑LPS heptosyltransferase 2 |
| Reads supporting (aligned to +/- strand): ref base A (5/17); new base G (2/2); total (7/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
CCTGCAGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTGAGCAGTGT > KT2440/406937‑407095 | ccTGCAGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAgg < 2:227929/135‑1 (MQ=255)ccTGCAGAAGGAGGCCGGGGTGAGCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAgg < 2:76880/135‑1 (MQ=255) aGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACGGGTACt > 4:104947/1‑134 (MQ=255) aGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACGGGTACTg > 4:109419/1‑135 (MQ=255) aGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACt > 6:80589/1‑134 (MQ=255) aGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg < 2:243386/135‑1 (MQ=255) aGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg < 1:241212/135‑1 (MQ=255) aGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGACGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg < 5:134578/135‑1 (MQ=255) aaGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACGGGTACTGca < 3:104947/135‑1 (MQ=255) aaGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACGGGTACTGca < 3:109419/135‑1 (MQ=255) gaggCCGGGGTGATCGATGCGGCACAGTTTGCCGATGACCGCCGTGAAGTGCGGCGGATCTGGGGTGAGTGGGGTTTAAGCGGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAg < 6:134408/135‑1 (MQ=255) gaggCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAg < 2:144482/135‑1 (MQ=255) ggggTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTGa < 7:135387/136‑1 (MQ=255) ggggTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGGTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTg < 4:127651/135‑1 (MQ=255) gggTGTTGGTTGCGGGACAGTTTGCCGATGTTCGCGGTGAAGTGCGGCGGATGTGTGGTGAGTTGGGTTTAAGCTGATAGCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTTCTGCAGAGGCCGTGa < 4:148009/135‑1 (MQ=255) gggTGATCGATGCGGCACAGTTTGCCGATGAACTCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTGa < 5:196806/135‑1 (MQ=255) gggTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTGa < 5:80589/135‑1 (MQ=255) cGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTGAGCAgtgt < 2:23947/135‑1 (MQ=255) gCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg > 2:78079/1‑100 (MQ=255) gCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg < 1:78079/100‑1 (MQ=255) gCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg > 5:84554/1‑90 (MQ=255) gCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg < 6:84554/90‑1 (MQ=255) aCCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg < 5:189056/45‑1 (MQ=255) aCCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTg > 6:189056/1‑45 (MQ=255) tATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTg > 5:57780/1‑44 (MQ=255) tATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTg < 6:57780/44‑1 (MQ=255) | CCTGCAGAAGGAGGCCGGGGTGATCGATGCGGCACAGTTTGCCGATGAACGCCGTGAAGTGCGGCGGATCTGGGGTGAGTTGGGTTTAAGCTGATACCGTGTTGGCTTATTCGCGGGCAAGCCCGCTCCCACAGGTACTGCACAGGCCCTGAGCAGTGT > KT2440/406937‑407095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |