Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 407,297 A→T 7.1% Q22L (CAG→CTG)  rfaF → ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440407,2970AT 7.1% 167.3 / 2.2 55Q22L (CAG→CTG) rfaFADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base A (27/23);  new base T (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAATATAAAGAGGGGAGGCCAGGCTGTACCGCAGCCTGTAGCCTCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTGCGCCAGGCCTTGAGCTTCCCGCTCGGCCACGGCGCGCTGGAGCT  >  KT2440/407164‑407429
                                                                                                                                     |                                                                                                                                    
cTGAATATAAAGAGGGGAGGCCAGGCTGTACCGCAGCCTGTAGCCTCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAg                                                                                                                                     >  1:112173/1‑135 (MQ=255)
          agagGGGAGGCCAGGCTGTACCGCAGCCTGTAGCCGCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGTAGACCCTGTTCCAGTGCCTGAAAc                                                                                                                           <  3:197772/135‑1 (MQ=255)
                gAGGCCAGGCTGTACCGCAGCCTGTAGCCTCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGCCCCTGTTCCCGTGCCTGAAACagcagc                                                                                                                     >  1:262779/1‑135 (MQ=255)
                gAGGCCAGGCTGTACCGCAGCCTGTAGCCTCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACagcagc                                                                                                                     >  1:257701/1‑135 (MQ=255)
                    ccAGGCTGTACCGCAGCCTGTAGCCTCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCAcccc                                                                                                                >  7:16594/1‑136 (MQ=255)
                        gCTGTACCGCAGCCTGTAGCCTCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACt                                                                                                            <  7:269672/136‑1 (MQ=255)
                                gCAGCCTGTAGCCTCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGAt                                                                                                     >  3:121109/1‑135 (MQ=255)
                                    ccTGTAGCCTCACACAGCTTTTCTGGACTAAGCATGAGAATACAGATCATTTGCCCCAGATGGGTCGGAGACATGGTGATCGAGCAGACACTGTTCCAGTGCCTTAAACAGCAGAACCAACACTTCCTGATCGAc                                                                                                 >  6:108730/1‑135 (MQ=255)
                                                 cGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGCCCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGATGGCCCCt                                                                                     >  4:263394/1‑134 (MQ=255)
                                                 cGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCCCCCCGACTGCGTGAGCGACGTGCTGGCCCCt                                                                                     >  4:275074/1‑134 (MQ=255)
                                                 cGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTg                                                                                    >  8:103217/1‑135 (MQ=255)
                                                 cGAGAGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCt                                                                                     >  6:156304/1‑134 (MQ=255)
                                                      gTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATgg                                                                               <  2:112173/135‑1 (MQ=255)
                                                          cTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTCATCGACGTGCAGGCCCCTGAATGGAgcc                                                                           >  6:71310/1‑135 (MQ=255)
                                                           tGGACTAAGCATGATAATCCTGATCATTGGCCGCAGCTGGGTCGGCGAAATGGTTATGGCGCTGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAgcc                                                                           <  2:262779/134‑1 (MQ=255)
                                                           tGGACTAAGCATGAGAATACTGATCATTGGCCCCTGCTGGGGCGGCGACGTGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAgcc                                                                           <  5:206729/134‑1 (MQ=255)
                                                           tGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAgcc                                                                           <  4:136296/134‑1 (MQ=255)
                                                                   gCATGAGAATACTGATCATTGGCCCCAGCGGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATc                                                                   <  5:133915/134‑1 (MQ=255)
                                                                     aTGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCt                                                                 >  2:9258/1‑134 (MQ=255)
                                                                     aTGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCt                                                                 >  1:178444/1‑134 (MQ=255)
                                                                     aTGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTc                                                                >  4:197865/1‑135 (MQ=255)
                                                                     aTGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCg                                                               >  7:84435/1‑136 (MQ=255)
                                                                      tGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              <  8:155446/136‑1 (MQ=255)
                                                                      tGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATAGACGTGCTGGCCCCTGAATTTAGACTGCCGATCCTCGa                                                              <  8:16594/136‑1 (MQ=255)
                                                                      tGAGAATACTGATCATTGGCCCCAGCGGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              <  7:86024/136‑1 (MQ=255)
                                                                       gagaATCCTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              <  2:257701/135‑1 (MQ=255)
                                                                       gagaATACTGATCATTGGCCCCAGTTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              <  2:46753/135‑1 (MQ=255)
                                                                       gagaATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              <  5:257991/135‑1 (MQ=255)
                                                                         gaATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAg                                                             <  1:71341/134‑1 (MQ=255)
                                                                                  tCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCt                                                    <  4:132518/134‑1 (MQ=255)
                                                                                    aTTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTg                                                                                                                               <  6:78186/57‑1 (MQ=255)
                                                                                    aTTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTg                                                                                                                               >  5:78186/1‑57 (MQ=255)
                                                                                    aTTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTgg                                                                                          >  1:22974/1‑94 (MQ=255)
                                                                                    aTTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTgg                                                                                          <  2:22974/94‑1 (MQ=255)
                                                                                    aTTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGaa                                                 >  6:219611/1‑135 (MQ=255)
                                                                                    aTTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGaa                                                 >  5:5945/1‑135 (MQ=255)
                                                                                    aTTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTCAAACAGCAGCACCCCGACTGCCTGATCGACGTCCTGGCCCCAGAATGGAGCCGGCCGATCCTCGACCGCAAGCCGGaa                                                 >  5:17803/1‑135 (MQ=255)
                                                                                      tGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGt                                               >  1:76248/1‑135 (MQ=255)
                                                                                       ggCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGt                                                                                                                                    >  5:85897/1‑49 (MQ=255)
                                                                                       ggCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGt                                                                                                                                    <  6:85897/49‑1 (MQ=255)
                                                                                        gCCCCAGCTGGGTCGGCGACTTGGGGAGGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTgc                                             <  3:145975/135‑1 (MQ=255)
                                                                                         ccccAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTgcg                                            >  3:60211/1‑135 (MQ=255)
                                                                                         ccccAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTgc                                             <  1:44560/134‑1 (MQ=255)
                                                                                         ccccAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTgc                                             <  3:88733/134‑1 (MQ=255)
                                                                                                       gCGACATGGTGATGGCGCAGACCCTGTTCCTGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              <  3:227262/103‑1 (MQ=255)
                                                                                                       gCGACATGGTGATGGCGCAGACCCTGTTCCTGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              <  3:231132/103‑1 (MQ=255)
                                                                                                       gCGACATGGTGATGGCGCAGACCCTGTTCCTGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              >  4:231132/1‑103 (MQ=255)
                                                                                                       gCGACATGGTGATGGCGCAGACCCTGTTCCTGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              >  4:227262/1‑103 (MQ=255)
                                                                                                                    ggCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTGCGCCAGGCCTTGAGCTTCCCGCTCGGCCa                 <  5:219611/135‑1 (MQ=255)
                                                                                                                           aCCCTGTTCCAGTGCCTGAAACAGCAGCACCCAGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTGCGCCAGGCCTTGAGCTTCCCGCTCGGCCACGgcgcg          >  5:251586/1‑135 (MQ=255)
                                                                                                                             ccTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTGCGCCAGGCCTTGAGCTTCCCGCTCGGCCACGGCGCGCt        >  2:196787/1‑135 (MQ=255)
                                                                                                                                  tCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTGCGCCAGGCCTTGAGCTTCCCGCTCGGCCACGGCGCGCTGGAGc   >  1:91262/1‑135 (MQ=255)
                                                                                                                                  tCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTGCGCCAGGCCTTGAGCTTCCCGCTCGGCCACGGCGCGCTGGAGCt  >  7:27937/1‑136 (MQ=255)
                                                                                                                                  tCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTAGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              <  6:187556/76‑1 (MQ=255)
                                                                                                                                  tCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTAGCCCCTGAATGGAGCCGGCCGATCCTCGa                                                              >  5:187556/1‑76 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CTGAATATAAAGAGGGGAGGCCAGGCTGTACCGCAGCCTGTAGCCTCCCCGAGCGTTTCTGGACTAAGCATGAGAATACTGATCATTGGCCCCAGCTGGGTCGGCGACATGGTGATGGCGCAGACCCTGTTCCAGTGCCTGAAACAGCAGCACCCCGACTGCGTGATCGACGTGCTGGCCCCTGAATGGAGCCGGCCGATCCTCGAGCGCATGCCTGAAGTGCGCCAGGCCTTGAGCTTCCCGCTCGGCCACGGCGCGCTGGAGCT  >  KT2440/407164‑407429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑