| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 752,516 | A→C | 60.0% | intergenic (+438/‑396) | CJ019_00719 → / → CJ019_00721 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 752,516 | 0 | A | C | 60.0% | ‑1.9 / 5.7 | 13 | intergenic (+438/‑396) | CJ019_00719/CJ019_00721 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base C (6/2); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-01 | |||||||||||
TAGCTTTCAGGTGCCTGATTCGACTCCTGTCTCCCCATTTACGCCGCTACCAACCTTCAACTAGATCTTGATACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCCCCCCAAACCTTGCATTGCAGCCCCCACGACGCTACCGTGCTCCAGTCGCGGCCAACTCCGCGACCGGGTTTGGCGACCCGAACGAAAAGGCGCATCGGCGCCCCCGACCACGATTGCAGGCGCTTTTTTTGCG > KT2440/752385‑752642 | tAGCTTTCAGGTGCCTGATTCGACTCCTGTCTCCCCATTTACGCCGCTACCAACCTTCAACTAGATCTTGATACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCCCCCCAAAcc < 3:88577/134‑1 (MQ=255) aaGGTGCCTGATTCGACTCCTGTCTCCCCATTTACGCCGCTACCAACCTTCAACTAGATCTTGATACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCCCCCCAAACCttgcatt < 3:56002/133‑1 (MQ=255) cAACTAGATCTTGATACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCCCCCCACCCCGTGCATTGCAGCCCCCACGCCGCTACGGTGCTCCAGTCGCGGCCCCCTCCGCGCCCggg > 7:193274/1‑136 (MQ=255) aCTAGATCTTGATACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCCCCCCAACCCTTGCATTGCAGCCCCCACGACGCTACCGTGCTCCAGTCGCGGCCCACTCCGCGCCCGGGtt > 8:234918/1‑136 (MQ=255) aCTAGATCTTGATACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCCCCCCAAACCTTGCATTGCAGCCCCCCCGACGCTACCGTGCTCCAGTCGCGGCCACCTCCGCGACCGGGtt > 7:193454/1‑136 (MQ=255) aCTAGATCTTGATACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCAGCGCCCCAACCCTTGCATTGCCGCCCCCACGACGCTACCGTGCTCCCGTCGCGGGCGCCTCCGCGCCCGGGt > 4:209841/1‑135 (MQ=255) tACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCCCCCCAACCCTTGCATTGCAGCCCCCACGACGCTACCGTGCTCCAGTCGCGGCCGCCTCCGCGACCGGGTTTGGCGCCCcgac > 8:217307/1‑135 (MQ=255) ccACCCCGTTTTCCCCGCGGTTCCAAACAACCCCCCGCGCCCATCCCCACCCCCCCACCCGTTCCATGGCCCCCCCCACGCCGCTACCGTGCTCCAGTCGCGGCCAACTCCGCGACCGGGTTTGGCGACCcgaacg < 7:148723/136‑1 (MQ=255) cGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCGCCCCCACCCTTGCATTGCAGCCCCCCCGACGCTACCGGGCTCCAGTCGCGGCCACCGCCGCGCCCGCGGTTGGCGCCCcgatcgcccag > 4:28184/1‑130 (MQ=255) ccAGCGTCCATTCGCATCCCCCCACACCTTGCATTGCAGCCCCCACGACGCTACCGTGCTCCAGTCGCGGCCAACGCCGCGCCCGGGTGTGGCGCCCCGAACGCAAAGGCGCATCGGCGCCCCCGCCCACGATTg > 3:214564/1‑135 (MQ=255) gCATCCCCCCAAACCTTGCATTGCAGCCCCCACGACGCTACCGTGCTCCAGTCGCGGCCAACTCCGCGACCGGGTTTGGCGACCCGAACGAAAAGGCGCATCGGCGCCCCCGACCACGATTGCAGGCGCgtttttt > 7:35935/1‑136 (MQ=255) cccgcccccaAACCCTTCCATTCCTGCCCCCCGGTCGGTCCCGGGATCCTGGCGGGGCCACCCCCGCGACCGGGTTTGGCGACCCGAACGAAATGGCGCATCGGCGCCCCCGACCACGATTGCAGGCGCtttttt < 4:152545/131‑1 (MQ=255) ccccccAACCCTTGCATTGCAGCCCCCACGCCGCTACCGTGCTCCAGTCGCGGCCAACTCCGCGCCCGGGTTTGGCGACCCGCACGCAAAGGCGCATCGGCGCCCCCGACCCCGATTGCAGGCGCgtttttttgg > 3:66710/1‑132 (MQ=255) | TAGCTTTCAGGTGCCTGATTCGACTCCTGTCTCCCCATTTACGCCGCTACCAACCTTCAACTAGATCTTGATACCAACCCGTTTTAGCCGACGTTTCCTACAACTCCCAGCGTCCATTCGCATCCCCCCAAACCTTGCATTGCAGCCCCCACGACGCTACCGTGCTCCAGTCGCGGCCAACTCCGCGACCGGGTTTGGCGACCCGAACGAAAAGGCGCATCGGCGCCCCCGACCACGATTGCAGGCGCTTTTTTTGCG > KT2440/752385‑752642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |