Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	778,930	C→G	23.6%	A16P (GCA→CCA)	CJ019_00742 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	778,930	0	C	G	23.6%	102.5 / 3.5	48	A16P (GCA→CCA)	CJ019_00742	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (6/30);  new base G (10/2);  total (16/32)
Fisher's exact test for biased strand distribution p-value = 5.90e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGCTCTGTTTGCGCAATTCGGCCTGGGTATGATTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTG  >  KT2440/778795‑779058                                                                                                                                        |                                                                                                                                 ccAGGCTCTGTTTGCGCAATTCGGCCTGGGTATGAGTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGc                                                                                                                                  <  8:111337/136‑1 (MQ=255)   aGGCTCTGTTTGCGCAATTCGGCCTGGGTATGATTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGc                                                                                                                                  <  4:140803/134‑1 (MQ=255)            ttGCGCAATTCGGCCTGGGTATGATTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGGGCGTGTgggggg                                                                                                                        >  1:195160/1‑133 (MQ=255)                          cTGGGTATGATTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATgacga                                                                                                          <  1:165896/135‑1 (MQ=255)                          cTGGGTATGATTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATgacga                                                                                                          <  6:112086/135‑1 (MQ=255)                               tATGATTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGAt                                                                                                     >  5:138820/1‑135 (MQ=255)                                aTGATTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATg                                                                                                    <  2:207637/135‑1 (MQ=255)                                   aTTGAACTGGGCGAACAGCTTCCACAGCATCAGGCCCAGCTGGTTCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCTGGAGAGTCGCGGGTGCTTGTTGGGCGGAACGCAATGACTACGGATGAGTa                                                                                                <  7:222205/136‑1 (MQ=255)                                          tGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGGATGTGGGGCGGCACGCAATGACCCCGGAAGAGTAAGGTTg                                                                                          >  2:49110/1‑135 (MQ=255)                                          tGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGGGGGTGTGGGGCGGGCCGCAATGACGCCGGATGAGTAAGGTTgg                                                                                         >  7:110994/1‑136 (MQ=255)                                            ggCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGGTTGTGGGGCGGACCGCCATGACGCCGGATGCGTAAGGTTgg                                                                                         >  4:158778/1‑134 (MQ=255)                                            ggCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTgg                                                                                         <  7:263376/134‑1 (MQ=255)                                                 aCAGCTGCCACAGCAGCAGGCCCAGCAAGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGc                                                                                   <  1:141761/135‑1 (MQ=255)                                                        ccACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGGGGGTGTGGGGCGGCCCGCCATGGCGCCGGATGAGTAAGGTTGGGGAAGCGTTGCTc                                                                            >  3:171864/1‑135 (MQ=255)                                                         cacaGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGGGGTTGTGGGGGGGACCGCAATGCCGCCGGATGAGTAAGGTTGGTCAAGCGGTGATcc                                                                           >  1:205897/1‑135 (MQ=255)                                                                gcagGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGGGGGTGGGGGGCGGCACGGCATGACGCCGGTTGAGTTAGGTTGGTCAAGCGTTGATCCTGCGATTg                                                                   >  8:165813/1‑136 (MQ=255)                                                                           agGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGGGTTGGGGGGGGCACGCCAAGACGACGGATGAGTAAGGTTGGTGAAGCGTTGATGCTGCGATTGGGGTGGCgct                                                         >  1:104864/1‑133 (MQ=255)                                                                            ggATCAGGGCCAGCAGCTCCAGCGCACCCTTGATCGACGCGCGCATGAGAGTCGCGGGTGCTTGTGCGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATg                                                        <  4:9979/135‑1 (MQ=255)                                                                            ggATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATg                                                        <  3:161104/135‑1 (MQ=255)                                                                                cAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAgg                                                   <  8:186822/136‑1 (MQ=255)                                                                                 aGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGGTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAgg                                                   >  8:165965/1‑135 (MQ=255)                                                                                 aGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGGGCATGTGGGGCGGAACCCAATGACGCAGGATGAGTAACGGTGGTGAAGCGTTGCTCCTGGGAATGGGCGGGCGGTGGGAgg                                                   >  2:86823/1‑135 (MQ=255)                                                                                  gggCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAgg                                                   <  3:220318/134‑1 (MQ=255)                                                                                  gggCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAgg                                                   <  6:91157/134‑1 (MQ=255)                                                                                  gggCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCGTGTGGGGCGGCACGCCCAGCCGCCGGCTGAGTATGGGTGGGGCAGCGTTGCTGCGGCGGTTGGGGGGGGGAGGGCAggg                                                  >  5:72736/1‑135 (MQ=255)                                                                                   ggCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGGCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGa                                                 <  6:264458/135‑1 (MQ=255)                                                                                           gcgccagcgcCCCCTTGATCGACCCGCGCAGGAGAGTCGAGTGTGCATTTGGGGCGGTCCGCCATGAAGCCGGTTTAGTAAGGTTGGTACAGCCTTGGTACTGCGATTTGGCTGGCGATGGCCGGGAATgctagt                                         >  6:268320/1‑130 (MQ=255)                                                                                           gcgccagcgcACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGGTTTTGGGGCGGAACGCAAAGACCACGGATGAGTTAGGTTGGTCTAGCGTTGATACTGCGATTGGGCTGGCGCTGGCCTGGAAATATCGt                                         >  6:14644/1‑135 (MQ=255)                                                                                           gcgccagcgcACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGt                                         <  6:124651/135‑1 (MQ=255)                                                                                            cgccagggcAGCGTTGATCGACGCGCGCAGGAGAGTGGGGGGTGCTTGTGTGCCGGAACGCTATGAGGACGGATGAGTTAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGTCAGGGAATGATCGTGc                                       <  8:228292/136‑1 (MQ=255)                                                                                            cgccagcgcACCCTTGATCGAGCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGCCGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGc                                       <  7:10249/136‑1 (MQ=255)                                                                                             gccagcgcACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGc                                       <  6:57260/135‑1 (MQ=255)                                                                                               cagcggACCCTTGATCGACCCGCGCAGTAGAGTCGCGGGTGGTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGGTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCAc                                     <  5:78996/135‑1 (MQ=255)                                                                                               cagcgcACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCAc                                     <  4:163971/135‑1 (MQ=255)                                                                                                                  ccGCGCAGGCGAGTCGCGGGGGCGTGGGGGGCGGAACGCAATGACGACGGCTGAGTCAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCGGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCgg                  <  6:182958/135‑1 (MQ=255)                                                                                                                    gcgcAGGAGAGTCGCGGGTGGGTGTGGGGGGGGACGCAATGACGACGGGTGGTTAATGTTGGTGCAGCGTTGATGCTGCGATTGGGCTGGGGTTGGCAGGGATTGATCGTGCCCCGTGGGTGCCGGCGCCCGGCt                >  2:268401/1‑135 (MQ=255)                                                                                                                    gcgcAGGAGAGTCGCGGGTGCTTGTGGGGCGGGACGCAATGTCGCCGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGCATGATCGTGCACGGTGGGTGCCGGCGCCCGGCt                >  3:54898/1‑135 (MQ=255)                                                                                                                     cgcAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCtt               <  6:138820/135‑1 (MQ=255)                                                                                                                            gcggcgcgGGGGCGTGGGGGGCGGAACGCAATGACGACGGCTGAGTAAGGTTGGGCACGCGTTGAGCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCAcc        <  5:152247/131‑1 (MQ=255)                                                                                                                                 gggggTGCTTGTGGGGCTGAACGCAATGACGACGGATGAGTAAGGCTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTGGCGCACCGCGGTg  <  8:114984/134‑1 (MQ=255)                                                                                                                                 gcgGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  7:269738/136‑1 (MQ=255)                                                                                                                                 gcgGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  8:110994/136‑1 (MQ=255)                                                                                                                                 gcgGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGGAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  7:265850/136‑1 (MQ=255)                                                                                                                                 gcgGGTGCTTGGGGGGCGGACCGCAAGGACGCCGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  7:231830/136‑1 (MQ=255)                                                                                                                                 gcgGGTGCTTGGGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGGCAAGCGCTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  8:270032/136‑1 (MQ=255)                                                                                                                                  ggggTGGTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTGGGGGAAGCTTGGATCCTTGGATTGGGCTGGCGATGGCAGGGAATGATCGTGCAAGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  6:53394/134‑1 (MQ=255)                                                                                                                                  cgGGTGCTTGTGGGGCGGACCGCAATGACGACGGATGTGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  5:192404/135‑1 (MQ=255)                                                                                                                                  cgGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  3:179866/135‑1 (MQ=255)                                                                                                                                  cgGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGGGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  2:104864/135‑1 (MQ=255)                                                                                                                                  cgGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGGTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTg  <  3:131525/135‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 CCAGGCTCTGTTTGCGCAATTCGGCCTGGGTATGATTGAACTGGGCGAACAGCTGCCACAGCAGCAGGCCCAGCAGGATCAGGGCCAGCAGCGCCAGCGCACCCTTGATCGACCCGCGCAGGAGAGTCGCGGGTGCTTGTGGGGCGGAACGCAATGACGACGGATGAGTAAGGTTGGTCAAGCGTTGATCCTGCGATTGGGCTGGCGATGGCAGGGAATGATCGTGCACGGTGGGTGCCGGCGCCCGGCTTGCGCACCGCGGTG  >  KT2440/778795‑779058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑