Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,523,327 T→A 6.9% intergenic (+97/‑16) ftsZ → / → lpxC Cell division protein FtsZ/UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,523,3270TA 6.9% 179.3 / 3.5 58intergenic (+97/‑16)ftsZ/lpxCCell division protein FtsZ/UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base T (28/26);  new base A (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGG  >  KT2440/1523199‑1523449
                                                                                                                                |                                                                                                                          
tCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGTGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCg                                                                                                                      >  1:129758/1‑135 (MQ=255)
     gCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                                                 <  6:240776/135‑1 (MQ=255)
     gCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                                                 <  4:104697/135‑1 (MQ=255)
           cTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCa                                                                                                            <  1:245372/134‑1 (MQ=255)
               gtcgtcAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGAt                                                                                                        <  2:76412/134‑1 (MQ=255)
                   tcAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTaaa                                                                                                    >  1:275479/1‑134 (MQ=255)
                              aaTGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTTCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTg                                                                                        >  7:111564/1‑135 (MQ=255)
                              aaTGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATAAAAGGTTGGGGCTGTTATCATCCCCAGCCTTTGTTGCTACCAGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGAATAAACAACGCACCCt                                                                                         >  6:103859/1‑134 (MQ=255)
                                    aTTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATa                                                                                 >  7:161249/1‑136 (MQ=255)
                                        aTCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCa                                                                              >  1:134450/1‑135 (MQ=255)
                                             gggTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGAGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGATGAATACCATCCGt                                                                         >  5:103512/1‑135 (MQ=255)
                                              ggTATAAAGGTGATTGGTATTCATCAAAGGTTGTGTCTGTTATCATCCCCAGCCTTTGTTGATAACTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATTATTAAACAACGCTCCCTGAAGAATACCATCCGTGc                                                                       <  8:259179/136‑1 (MQ=255)
                                               gTATAAAGGTGATTTGTATTCATCAAAGGTTGGGTCTGTTATGATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGc                                                                       <  8:276076/135‑1 (MQ=255)
                                               gTATAAAGGTGATTTGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGc                                                                       <  3:209882/135‑1 (MQ=255)
                                               gTATAAAGGTGATTGGTATTCATCAAAGGGTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGc                                                                       <  2:233248/135‑1 (MQ=255)
                                                    aaGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAg                                                                  <  5:230556/135‑1 (MQ=255)
                                                     aGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                                                 >  5:30523/1‑87 (MQ=255)
                                                     aGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                                                 <  6:30523/87‑1 (MQ=255)
                                                     aGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAgg                                                                 >  5:101886/1‑135 (MQ=255)
                                                      ggTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGgtg                                                               <  7:272964/136‑1 (MQ=255)
                                                           ttGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGt                                                          <  8:136180/136‑1 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATCCCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTcc                                                        >  7:276076/1‑135 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                                  <  6:186466/94‑1 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                                  >  5:186466/1‑94 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACACCGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTc                                                         >  5:195733/1‑135 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGt                                                          <  7:147760/134‑1 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGt                                                          >  6:22391/1‑134 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGt                                                          <  5:22391/134‑1 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGt                                                          >  8:147760/1‑134 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTc                                                         >  5:65975/1‑135 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTc                                                         >  6:267389/1‑135 (MQ=255)
                                                             ggTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGAGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTctgact                                                        >  8:229203/1‑131 (MQ=255)
                                                                 ttCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCa                                                     <  7:16603/135‑1 (MQ=255)
                                                                    atcaAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTcc                                                 >  7:90937/1‑136 (MQ=255)
                                                                       aaaGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                                              >  2:130034/1‑88 (MQ=255)
                                                                       aaaGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                                              <  1:130034/88‑1 (MQ=255)
                                                                        aaGGTTGGGTCTGTTATCATCGCCAGCCTTTGTTGATAGCTGTTCGCAATTTGCGGTGAAGCGGCCAATGACATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCggg                                              <  4:223961/135‑1 (MQ=255)
                                                                        aaGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCgg                                               <  5:267389/134‑1 (MQ=255)
                                                                            ttGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAACGGCCAATGCCATGATTAAACACCGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGga                                            >  4:206834/1‑133 (MQ=255)
                                                                                 tCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCgggg                                             <  6:193235/127‑1 (MQ=255)
                                                                                 tCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCgggg                                             >  5:193235/1‑127 (MQ=255)
                                                                                 tCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGt                                      >  1:213829/1‑134 (MQ=255)
                                                                                 tCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGt                                      >  3:212489/1‑134 (MQ=255)
                                                                                 tCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCAGTGCCACAGGTGACGGTCTGCACTCCCGGGAGAAgga                                      >  6:275153/1‑133 (MQ=255)
                                                                                         catcCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGAc                             <  5:194051/135‑1 (MQ=255)
                                                                                             cccAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCt                          >  4:112712/1‑134 (MQ=255)
                                                                                             cccAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTc                         >  8:91944/1‑135 (MQ=255)
                                                                                                aGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGc                     <  8:20271/136‑1 (MQ=255)
                                                                                                 gCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCTCCCTGAAGAATAGCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTATCTGACCCTCAAGc                     <  5:275153/135‑1 (MQ=255)
                                                                                                 gCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATGCCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGc                     <  1:104990/135‑1 (MQ=255)
                                                                                                                tGTTCGCAATTTGCGCAGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAcc                                                                               <  4:166383/62‑1 (MQ=255)
                                                                                                                tGTTCGCAATTTGCGCAGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAcc                                                                               <  4:163051/62‑1 (MQ=255)
                                                                                                                tGTTCGCAATTTGCGCAGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAcc                                                                               >  3:166383/1‑62 (MQ=255)
                                                                                                                tGTTCGCAATTTGCGCAGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAcc                                                                               >  3:163051/1‑62 (MQ=255)
                                                                                                                   tCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCg   <  4:212489/135‑1 (MQ=255)
                                                                                                                     gCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCg   <  7:153855/133‑1 (MQ=255)
                                                                                                                     gCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCg   >  8:153855/1‑133 (MQ=255)
                                                                                                                     gCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGATGCCAACgc  >  7:239467/1‑133 (MQ=255)
                                                                                                                                |                                                                                                                          
TCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGG  >  KT2440/1523199‑1523449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑