| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,692,266 | G→T | 100% | E320* (GAA→TAA) | cheB_1 → | Protein‑glutamatemethylesterase/protein‑glutamine glutaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,692,266 | 0 | G | T | 77.0% | 55.7 / 18.7 | 35 | E320* (GAA→TAA) | cheB_1 | Protein‑glutamatemethylesterase/protein‑glutamine glutaminase |
| Reads supporting (aligned to +/- strand): ref base G (7/1); new base T (13/14); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGAACAATGATGAATGATTTACCGATCGAAGG > KT2440/1692139‑1692396 | gggCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGCCCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCACGGCTGCTGCGGCTATTGATGCCGCCGGGTAAATccgc > 8:87744/1‑136 (MQ=255) cgACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCt < 5:136517/135‑1 (MQ=255) cGGTGCCCAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTggc > 1:6578/1‑134 (MQ=255) cGGTGCCCAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGa > 1:11311/1‑135 (MQ=255) cAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCAtt < 5:101639/135‑1 (MQ=255) gCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCtt > 3:67698/1‑135 (MQ=255) gcagGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCACGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAAttt > 6:114838/1‑133 (MQ=255) agGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGCCTGAAtttttg > 1:77785/1‑133 (MQ=255) cTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCaaa < 5:255393/135‑1 (MQ=255) cTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATg < 1:251710/134‑1 (MQ=255) aCCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGAc < 7:44576/133‑1 (MQ=255) ccATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGAc < 3:260834/132‑1 (MQ=255) ccATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGAc < 3:49617/132‑1 (MQ=255) aTTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAAt < 1:5064/135‑1 (MQ=255) gCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTa > 1:206673/1‑135 (MQ=255) ggCCCGCCGCGCGGTGTACGGCTTGCCCATGGCTGCTGCGGCTATTGTGGCCGCGGGTTAAAGCCCCCCGCTGGCTCGCATTGCCGGGCGCTTGAGTGAATTTTTCGCAAAATGAGGATTTGTATTCAAGCAcgc < 6:154173/135‑1 (MQ=255) cagcagCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATTAAGAAATGTATTGAATCAcgccgc > 6:249199/1‑135 (MQ=255) cagcagCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCGTGCCTGAATTTTTCGCAAAATGACGAAATGTATTGAATAAcgccg > 4:256188/1‑134 (MQ=255) gcagcGCGGTGTACGGCATGCCCAAGGCTGATGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCTTTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCa < 4:111348/135‑1 (MQ=255) gcgcGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGcc > 8:262958/1‑136 (MQ=255) gtagtgCATGCAAAAGGGTGCTGGGGCTATTGATGCGGCCGTGTAAATCCGCCGGCTGTAACGCTTTGCCGGGCGCTTGACTGAATTTTTCTAAAATTGACGAATTTTATTGAATCACGCCGCCAGGCCGGTAGt < 4:115348/130‑1 (MQ=255) gtACGGCATGCCCAAGGCTGCTGCGGCTATTGAGGCCGCCGTGTAAATCCGCCCGCTGGACCGCATTGCCGGGCGCTTGCCTGAATTTTTCGCAAAATCCCGAAATGTATTGAATCACGCCGCCAGGCCGGTAGt < 3:173188/135‑1 (MQ=255) cAAGGCTGCTGTGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGAAc > 7:21094/1‑135 (MQ=255) cAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATTACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGaa > 2:10015/1‑134 (MQ=255) cAAGGCTGCTGCGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACGGAATTTTTGGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGaa > 5:122898/1‑134 (MQ=255) cAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAAGTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTgca > 5:78159/1‑132 (MQ=255) cAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCAATGCCGGGCGCGTGACTGAATTTTTCGCAAAATGACGAAAAGTATTGAATCACGCCGCCAGGCCGGGTGTGATCGGGTGaa > 3:115348/1‑134 (MQ=255) ctgctgCGGCTATTGATGCCGCCGTGTAAATACGCCCGCTGGAACGCATTGCCGGGCGCTTGCCTGAATTTTTCGCAAAATTCCGAAATGTATTGAATCACGCCGCCAGGGCGGTAGTGATCGGGTGTTCAatgat > 7:58570/1‑136 (MQ=255) cGGCTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATAACGCCGCCAGGCCGGTAGTGATCGGGTGTAAAATGatgaatga > 7:122739/1‑136 (MQ=255) cGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTAGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGAACAATGatgaatg > 3:63833/1‑135 (MQ=255) cTATTGATGCCGCCGTGTAACACCGCCCGCGGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGAACAATGATGAATGAtt < 2:249514/135‑1 (MQ=255) cTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGAACAATGATGAATGAtt < 2:206673/135‑1 (MQ=255) cTATTGATGCCGCCGTGTAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGAACAATGATGAATGAtt < 1:10015/135‑1 (MQ=255) gccgTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGAACAATGATGAATGATTTACCGATCGaa > 2:32218/1‑135 (MQ=255) cgTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTTACTGAATTTTTAGCAAAATGACGAAATGTATTGAATCACCCCCCCCGGCCGGTAGTGATCGGGTGCACAATGATGAATGAATTACCGATCGAAgg > 6:194969/1‑135 (MQ=255) | GGGCCGCGACGGTGCCCAGGGCCTGAAGCTGATGCGTCAGCAGGGCTTTCTGACCATTGCCCAGGACCAGGCCAGCAGCGCGGTGTACGGCATGCCCAAGGCTGCTGCGGCTATTGATGCCGCCGTGGAAATCCGCCCGCTGGAACGCATTGCCGGGCGCTTGACTGAATTTTTCGCAAAATGACGAAATGTATTGAATCACGCCGCCAGGCCGGTAGTGATCGGGTGAACAATGATGAATGATTTACCGATCGAAGG > KT2440/1692139‑1692396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |