| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,720,951 | T→G | 56.1% | D90A (GAC→GCC) | plsB ← | Glycerol‑3‑phosphate acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,720,951 | 0 | T | G | 56.1% | 1.8 / 14.4 | 26 | D90A (GAC→GCC) | plsB | Glycerol‑3‑phosphate acyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (8/3); new base G (3/12); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01 | |||||||||||
GGGGTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGG > KT2440/1720819‑1721080 | ggggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg < 7:211219/136‑1 (MQ=255) gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg < 5:203642/135‑1 (MQ=255) ggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGTCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCggg < 3:155874/135‑1 (MQ=255) cccAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGTCAggg > 1:188909/1‑134 (MQ=255) acacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGGCAGGTAgaagaa > 8:190058/1‑136 (MQ=255) ctcaccggATTGATCTGCACATGTTATTCGGCATGCTGGCTGACGGCCGCCACCCGGCGCTCGCGCGTGGGGGGGGCGCCGCTTTTGTCCGGGGGGCCGAGCCAGTCCGGGGCGGGGGGCAGGTATAACAAcgcg < 4:17390/129‑1 (MQ=255) cTGACAGGAATGATCTGCGCATCTTCTTCGGCTTGGTGGCTGACGGCAGCAACCAGGCGCTCGAGCGGGGGGGGGGCGCCGTTTTTGTCCTGGCGGCCGAGCCAGTCGGGGGGGGGGGTCAGTTAGAAGAAcgcg < 4:18784/135‑1 (MQ=255) cTGACAGGAATGAGCTGCGCATCTTCTGCGGCATGCTGGCGGCCGGCAGCCACCTGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGTCAGGTAGAAGAAcgcg < 4:251879/135‑1 (MQ=255) gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCACTCCCGGTCGGGGGTCAGGTAGAAGAACgcggc > 4:258032/1‑135 (MQ=255) aTCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGc > 4:239520/1‑135 (MQ=255) gcgcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCTGTCCGGGGCGGGGGTCAGGTAGAAGAAcgcg < 6:108830/119‑1 (MQ=255) gcgcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGGCAGGTAGAAGAAcgcg > 5:108830/1‑119 (MQ=255) gcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTTTCCTGGCGGCCGAGCCACTCCGGGGAGCGGGGCAGGGATAAGAAACCCGGGGGGTACTGCAGgggg > 2:128366/1‑135 (MQ=255) gcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCCGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGc > 8:65512/1‑136 (MQ=255) tcttcGGCATGCGGGCGGCCGGCAGCCACCAGGCGCTCGAGGGTGGGCGGGGCGCCGCTTTTGTCCGGGCGGCGGAGCCAGGCCGGGGCGGGGGACAGGTACAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACg < 7:65512/135‑1 (MQ=255) gCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTAcc > 1:5850/1‑135 (MQ=255) ggCAGCCACCCGGCGCGCGAGCGGGGGGGGGGCGCCGTTTTTGGCGTGGCGGCGGAGCCGGTCGGGTGCGGGGGGCTGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGgcg < 1:58624/135‑1 (MQ=255) gCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGc > 4:105889/1‑135 (MQ=255) cccccaGGCGCGCGCGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCTGGCCGGGGCGGGGGGCAGGTAGACCACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCa < 4:183134/132‑1 (MQ=255) ccacccGGCGCGGGCGGGGGGGGGGGGCGCCGCTTTTGGCCGGGCGGCCGCGCCAGGCCGGGGCGGGGGGCTGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCa < 3:199321/135‑1 (MQ=255) ccaccaGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCGGACGGCTACCGGCAGCACCGGGCGCGGCa < 4:95473/135‑1 (MQ=255) ggcggggcgCCGCTGTTGGCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGGCAGGTAGACGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGt < 8:13086/136‑1 (MQ=255) gccgcTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCCGCCCCGCCTTGGTGCCCTCGGGGTCGAg > 6:40144/1‑135 (MQ=255) ggcCGAGCCGGGGCGGGGGGGGGGTCCGTTATATCACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCGTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGa < 3:50876/135‑1 (MQ=255) gcCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAg > 3:50687/1‑135 (MQ=255) aGCCAGGCCGGGGCGGGGGGCAGGTAGAAGCCCGCGGCGGGTGCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGcgcg < 6:59936/135‑1 (MQ=255) cgggggCGGGGGGCCGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGGCGGTGAGCGCGGGCGAgg < 3:214711/133‑1 (MQ=255) | GGGGTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGG > KT2440/1720819‑1721080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |