Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,805,251 C→T 6.9% D75N (GAT→AAT)  dmlR_4 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,805,2510CT 6.9% 186.1 / 3.4 58D75N (GAT→AAT) dmlR_4HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (32/22);  new base T (2/2);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

GTACAGCGCCATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAG  >  KT2440/1805117‑1805384
                                                                                                                                      |                                                                                                                                     
gTACAGCGCCATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATc                                                                                                                                       <  4:247546/135‑1 (MQ=255)
  aCAGCGCCATGAATCGAGTCAACATCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATTGCGCGCAGCGCTTCCTCACTGCCATCCt                                                                                                                                     <  4:233829/135‑1 (MQ=255)
     gcgcCATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTg                                                                                                                                    >  2:93129/1‑133 (MQ=255)
        ccATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGTCCACTGCACACGTCATGAGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGTCGCGCAGAGCTTCCTCACGGCCATCATCCAAGc                                                                                                                               >  4:148428/1‑135 (MQ=255)
        ccATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGc                                                                                                                               >  3:59871/1‑135 (MQ=255)
        ccATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCg                                                                                                                              >  8:237815/1‑136 (MQ=255)
         cATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCg                                                                                                                              >  2:64780/1‑135 (MQ=255)
         cATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGt                                                                                                                             <  7:231933/136‑1 (MQ=255)
            gAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTg                                                                                                                           <  5:146668/135‑1 (MQ=255)
             aaTCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTgg                                                                                                                          <  7:77662/135‑1 (MQ=255)
              aTCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTgg                                                                                                                          <  4:224981/134‑1 (MQ=255)
                   gTCACCAGCGGCACGATGAAGAGCTCTCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAAtgc                                                                                                                    >  2:68119/1‑135 (MQ=255)
                                  aTGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGcc                                                                                                    >  7:199225/1‑136 (MQ=255)
                                    gAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGccc                                                                                                   <  3:101891/135‑1 (MQ=255)
                                      aGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATTCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCg                                                                                                  <  4:46091/134‑1 (MQ=255)
                                        cgcTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATTCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCtt                                                                                              >  8:12755/1‑136 (MQ=255)
                                         gcTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATTCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTc                                                                                             <  7:205335/136‑1 (MQ=255)
                                         gcTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTc                                                                                             <  8:200805/136‑1 (MQ=255)
                                             gCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGc                                                                                           >  2:62930/1‑134 (MQ=255)
                                              ccGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCt                                                                                          >  8:161491/1‑134 (MQ=255)
                                               cGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGGAGCGCTTCCTCACGGCCATCCTCCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTGCGCGCa                                                                                        >  2:183947/1‑135 (MQ=255)
                                                gTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTAGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGCCTGCGCTCAgg                                                                                      >  7:257291/1‑136 (MQ=255)
                                                          gCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGACGCGCTACCTCCCGGCCATCCTGCAAGAGTTGGCAATGCTGCAGTAACGTCTGCCCGGCTTCGCTCAGGGTTACCcgg                                                                             >  5:9695/1‑135 (MQ=255)
                                                            acacGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTc                                                                                             >  6:17846/1‑117 (MQ=255)
                                                            acacGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTc                                                                                             <  5:17846/117‑1 (MQ=255)
                                                            acacGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCcggcg                                                                           >  4:64873/1‑135 (MQ=255)
                                                            acacGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCcggcg                                                                           >  3:123471/1‑135 (MQ=255)
                                                            acacGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCcggc                                                                            <  4:106815/134‑1 (MQ=255)
                                                            acacGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCAGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCcggcg                                                                           >  6:235158/1‑135 (MQ=255)
                                                            acacGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCAGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCcggcg                                                                           >  5:157129/1‑135 (MQ=255)
                                                             cacGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTACTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCcggcgg                                                                          <  1:39197/135‑1 (MQ=255)
                                                                gTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGtg                                                                       <  6:241090/135‑1 (MQ=255)
                                                                gTCATGAGCAACAAGCACTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGTAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGtg                                                                       <  3:148428/135‑1 (MQ=255)
                                                                   aTGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGtgctgc                                                                   >  8:185811/1‑136 (MQ=255)
                                                                   aTGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGtgctg                                                                    >  1:212542/1‑135 (MQ=255)
                                                                   aTGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGtgctg                                                                    >  4:153786/1‑135 (MQ=255)
                                                                    tGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGtgctg                                                                    <  3:112388/134‑1 (MQ=255)
                                                                     gcgcAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCACGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGggcgg                                                                    >  1:115483/1‑131 (MQ=255)
                                                                      cgcAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCg                                                                  >  5:61035/1‑134 (MQ=255)
                                                                        caacaaGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGAt                                                                >  5:70228/1‑134 (MQ=255)
                                                                             aGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATAcagcag                                                         >  8:246554/1‑136 (MQ=255)
                                                                                 cTTGGGTTCGCTGGCCAGGTCGCCCAGGGCGCGCAGCGCTTCCTCACGGCCATCCTGCACGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGgcg                                                      <  1:93129/135‑1 (MQ=255)
                                                                                      gTTCGCTGGCCAGGTCGCCCATGGCGAGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGttt                                                 >  5:275636/1‑135 (MQ=255)
                                                                                          gCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCa                                              <  3:82736/134‑1 (MQ=255)
                                                                                          gCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCa                                              <  4:59871/134‑1 (MQ=255)
                                                                                            tGGCCAGGTCGCCCATGGCGCGCACCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTGTGCCGGc                                           >  1:120625/1‑135 (MQ=255)
                                                                                             ggCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATTCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCATGCGCGTTTGCAGGCg                                          >  6:178889/1‑135 (MQ=255)
                                                                                                      gCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAg                                 >  4:28377/1‑135 (MQ=255)
                                                                                                           tGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACCGCAGGCGCGGTTGCAGGCGGGCCTCccgccg                              >  4:6798/1‑133 (MQ=255)
                                                                                                                   aGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGc                    >  1:153306/1‑135 (MQ=255)
                                                                                                                   aGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGGCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGc                    <  1:68119/135‑1 (MQ=255)
                                                                                                                     cgcTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACCGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCg                  >  3:188638/1‑135 (MQ=255)
                                                                                                                     cgcTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGAGACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGAGCTGCCgg                 >  8:244136/1‑136 (MQ=255)
                                                                                                                       cTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCt               <  7:237815/136‑1 (MQ=255)
                                                                                                                             cACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGAtggg         >  8:136837/1‑134 (MQ=255)
                                                                                                                                   cATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGGCGGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATg    <  2:252845/135‑1 (MQ=255)
                                                                                                                                    aTCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGa   <  1:62930/135‑1 (MQ=255)
                                                                                                                                    aTCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTGCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGa   <  4:122943/135‑1 (MQ=255)
                                                                                                                                     tCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAg  <  2:87828/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GTACAGCGCCATGAATCGCGTCACCAGCGGCACGATGAAGCGCTCGCCGTAGGCCACTGCACACGTCATGCGCAACAAGCCCTTGGGTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAG  >  KT2440/1805117‑1805384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑