Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,831,738 T→G 30.4% S163R (AGC→CGC)  CJ019_01748 ← NAD(P)H dehydrogenase (quinone)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,831,7380TG30.4% 20.0 / 2.5 28S163R (AGC→CGC) CJ019_01748NAD(P)H dehydrogenase (quinone)
Reads supporting (aligned to +/- strand):  ref base T (4/14);  new base G (2/7);  total (7/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GGCTTTCTAGCCACGCGCCGCCTCCAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGCCGTGGTGCATCAGCGGCAGCATCATCGACAGCAGGGTGGTTTCCTGGCCGCCGTG  >  KT2440/1831613‑1831870
                                                                                                                             |                                                                                                                                    
ggCTTTCTAGCCACGCGCCGCCTCCAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCTGGCGCCAt                                                                                                                              >  2:20922/1‑134 (MQ=255)
   tttCTAGCCACGCGCCGCCTCCAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCTGGCGCCATAACGc                                                                                                                         >  7:96336/1‑136 (MQ=255)
      cTAGCCACGCGCCGCCTCCAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCTGGCGCCATAAGGCGt                                                                                                                       <  8:52091/135‑1 (MQ=255)
        aGCCACGCGCCGCCTCCAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCAGGCGCCATAAGGCGTgc                                                                                                                     >  2:228561/1‑135 (MQ=255)
             cgcgCCGCCTCCAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCGGGGGCCCTAACGCGTgccgccc                                                                                                                >  3:162220/1‑134 (MQ=255)
             cgcgCCGCCTCCAGGGCCTTGGCCGTGGGCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCGTGCGTGGTGGCGGGCGCCATAAGGCGGCCCGccac                                                                                                               <  8:233459/136‑1 (MQ=255)
                 ccgccTCCAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCGGGCGCCATAACGCGTGcccccccccc                                                                                                            >  2:47174/1‑133 (MQ=255)
                        ccgggCCTTGGCCGGGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGGGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGGGTGGTGGCTGGCGCAATAAGGCGTGCCGCCACCACGGGtctc                                                                                                     <  2:202950/133‑1 (MQ=255)
                        cAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGGGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGGGGGGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGtctc                                                                                                     <  5:138976/135‑1 (MQ=255)
                        cAGGGCCTTGGCCGGGGTCGCCCGGCGTGGGCCCCGGGCGCGGCACAGGGCGATTTCGTGGGGGGCGAGTGCGCGCTTGCCATCGGCGCCGGCGTGGTGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGtctc                                                                                                     <  2:21030/135‑1 (MQ=255)
                                  gcggTGGTCGCCAGGCGTGGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGGGGTGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGcc                                                                                           <  8:27894/132‑1 (MQ=255)
                                  gCCGTGGTCGCCTGGCGTTGGCCGAGGGCGCGGCACAGGGCGTTTTCGTGTGGGGCGATTTCGCGCTTGCCATCGGCGCCTGCGGGGGGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTg                                                                                             <  3:211124/133‑1 (MQ=255)
                                  gCCGTGGTCGCCAGGCGGTGGCCCAGGGCGCGGCACTGGGCGATTTCGTGTGGGGCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCg                                                                                          <  8:89652/136‑1 (MQ=255)
                                   ccGGGGGCGCCCGGCGGTGGCCCAGGGGGGGGCACAGGGCGATTTCGTGGGGGGCGTGTTCGCGCTGGCCATCGGCGCCTGCGGGGGGGCGGGCGCCATACGGCGGGCCGCCACCACGGGTCTCGAGCAGTGCCg                                                                                          <  1:13535/135‑1 (MQ=255)
                                              aGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTGGCCATCGGCGCCTGCGGGGGGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAg                                                                               <  4:59943/135‑1 (MQ=255)
                                              aGGCGTGGGCCCAGGGCGCGGCACCGGGCGATTTCGTGTGGGGCGAGTTCGCGCTTGCCAGCGGCGCCGGCGGGGGGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAgg                                                                              <  7:220598/136‑1 (MQ=255)
                                              aGGCGGTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGGGGGGCGAGTGCGCGCTTGCCATCGGCGCCTGCGGGGGGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAg                                                                               <  5:123318/135‑1 (MQ=255)
                                                      gcaccgggTGCGGAACAGCGCCTATTGGTGCGGGGGGAGTCCGGGCTTGCTAGCGGGGCGTGCGGGGTGGGTGGCCCTATACGGGGGCCCGCAACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGcc                                                                       <  2:273593/130‑1 (MQ=255)
                                                      gCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGGGTGGTGGCTGGCGCAATACGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGcc                                                                       <  6:87046/135‑1 (MQ=255)
                                                           gggCGGGGCACTGGGCGATTTCGTGGGGGGCGTGTGCGCGCTTGCCATCGGCGCCTGCGTGGTGGCGGGCGCCATTAGGGGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCa                                                                  <  1:228561/135‑1 (MQ=255)
                                                                 ggCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTGGCCATCGGCGCCTGCGTGGTGGCGGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCa                                                            <  4:99987/135‑1 (MQ=255)
                                                                    acaGGGCGATTTCGGGGGGGGCGAGTTCGCGCTGGCCATCGGCGCCGGCGGGGGGGCTGGCGCCATAAGGCGGGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGc                                                         <  6:246033/135‑1 (MQ=255)
                                                                               tCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCTGGCGCCATAAGGCGTGCCGCCCCCACGGGTCTCGAGCAGTGCCGCCTCGCGGTAGGGCAGGCCCCTCACCAGCATGCCGTGGTGCAt                                               >  7:205816/1‑134 (MQ=255)
                                                                                     gggTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGCCGTGGTGCATCAGCgg                                         >  8:167964/1‑134 (MQ=255)
                                                                                           cgtgcgcgGTTGCCTTGGGCGCGTGCGTGGGGGGGGGCGCCATACGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGCCGTGGTGCATCAGCGGCAGcatc                                  <  1:47174/131‑1 (MQ=255)
                                                                                                       cccgcGGCGCCGGCGGGGGGGCTGGCGCCATAAGCCGGGCGGCCCCCACGGGGCGCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGCCGTGGTGCATCAGCGGCAGCATCATCGACAGCAgg                      <  1:182544/131‑1 (MQ=255)
                                                                                                              cgcCGGCGTGGGGGCGGGCGCCATAAGGCGGGCGGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGCCGTGGTGCATCAGCGGCAGCATCATCGACAGCAGGGTGGttt               <  1:20922/135‑1 (MQ=255)
                                                                                                              cgcCGGCGTGGGGGCGGGCGCCATAAGGCGGGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGCCGTGGTGCATCAGCGGCAGCATCATCGACAGCAGGGTGGttt               <  4:27033/135‑1 (MQ=255)
                                                                                                                 ctggggggtgggtgGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGCCGTGGTGCATCAGCGGCAGCATCATCGACAGCAGGGTGGTTTCCTg           <  7:46165/130‑1 (MQ=255)
                                                                                                                           gctggcGCCATAAGGCGTTCCGCCACCACCGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCCCCAATCCGTGGTGCATCAGCGGCAGCATAATCGACACCCAGGTGGTTTACATGCCCCCGTg  >  4:170663/1‑135 (MQ=255)
                                                                                                                             |                                                                                                                                    
GGCTTTCTAGCCACGCGCCGCCTCCAGGGCCTTGGCCGTGGTCGCCAGGCGTTGGCCCAGGGCGCGGCACAGGGCGATTTCGTGTGGGTCGAGTTCGCGCTTGCCATCGGCGCCTGCGTGGTGGCTGGCGCCATAAGGCGTGCCGCCACCACGGGTCTCGAGCAGTGCCGACTCGCTGTAGGGCAGGCCCATCACCAGCATGCCGTGGTGCATCAGCGGCAGCATCATCGACAGCAGGGTGGTTTCCTGGCCGCCGTG  >  KT2440/1831613‑1831870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑