Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,055,416	A→T	8.0%	N425I (AAT→ATT)	CJ019_01952 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,055,416	0	A	T	8.0%	151.0 / 3.8	50	N425I (AAT→ATT)	CJ019_01952	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base T (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

GGCCTGCCGGCCCTGCTCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCGCTGCTGCAGACGCT  >  KT2440/2055292‑2055545                                                                                                                             |                                                                                                                                  ggCCTGCCGGCCCTGCTCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGt                                                                                                                          >  2:82314/1‑134 (MQ=255) ggCCTGCCGGCCCTGCTCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTc                                                                                                                         <  2:200605/135‑1 (MQ=255)   ccTGCCGGCCCTGCTCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAAc                                                                                                                      <  8:61371/136‑1 (MQ=255)                cTCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTcc                                                                                                          <  3:520/135‑1 (MQ=255)                cTCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTcc                                                                                                          <  1:84307/135‑1 (MQ=255)                 tCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTcc                                                                                                          <  2:182588/134‑1 (MQ=255)                 tCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGGGTTCTcc                                                                                                          <  1:165908/134‑1 (MQ=255)                   aGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTccgac                                                                                                       >  5:49946/1‑135 (MQ=255)                   aGCGCCCGTGACTACGAAGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTccgac                                                                                                       >  6:227651/1‑135 (MQ=255)                        ccGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCcgacccgacc                                                                                                 >  7:173229/1‑136 (MQ=255)                        ccGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCcgacccgacc                                                                                                 >  8:268367/1‑136 (MQ=255)                          gTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACATCCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATAGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCt                                                                                                <  1:116619/135‑1 (MQ=255)                           tGACTACGACGATGTGTACGGCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTg                                                                                               <  2:52472/135‑1 (MQ=255)                             aCTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTggg                                                                                             >  6:23874/1‑135 (MQ=255)                                    cgaTGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTcc                                                                                     <  7:161506/136‑1 (MQ=255)                                     gaTGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTcc                                                                                     <  2:153875/135‑1 (MQ=255)                                         tgtACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGCCCTGGGGTGTGTCCGa                                                                                   >  5:241419/1‑133 (MQ=255)                                          gtACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTcc                                                                                                          >  6:7793/1‑109 (MQ=255)                                          gtACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTcc                                                                                                          <  5:7793/109‑1 (MQ=255)                                            aCGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAgg                                                                              >  3:135687/1‑135 (MQ=255)                                                        gCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGGCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGAcc                                                                  <  6:241419/135‑1 (MQ=255)                                                        aagACTTTGCCTGGGACAACCAGTCCTTTTTATTAGGAAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGCCATGTTCGAcc                                                                  >  4:256461/3‑135 (MQ=255)                                                           aCTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTcc                                                                                                          >  4:134464/1‑92 (MQ=255)                                                           aCTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTcc                                                                                                          <  3:134464/92‑1 (MQ=255)                                                           aCTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACcgcg                                                               >  4:222093/1‑135 (MQ=255)                                                            cTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCgg                                                              >  8:69543/1‑135 (MQ=255)                                                            cTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCgg                                                              >  4:183721/1‑135 (MQ=255)                                                                            cAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGcc                                              <  3:109087/135‑1 (MQ=255)                                                                            cAGTCAGGGTTCTTCGGCAAACAGCGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACAAGCACATGATCGACCGCGGCAACGAAGAGCTGGc                                               >  6:88582/1‑134 (MQ=255)                                                                             aGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTGCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCa                                             <  5:166133/135‑1 (MQ=255)                                                                              gTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACAAGGCCATGGTCGCCCGCGGCACCGAAGCGCTGGCCaa                                            >  2:274956/1‑135 (MQ=255)                                                                                          cGGCAACCAGGGGATGACCACCTTCATCGGCCGTATTGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCa                                         <  2:44806/126‑1 (MQ=255)                                                                                          cGGCAACCAGGGGATGACCACCTTCATCGGCCGTATTGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCa                                         >  1:40604/1‑126 (MQ=255)                                                                                          cGGCAACCAGGGGATGACCACCTTCATCGGCCGTATTGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCa                                         <  2:40604/126‑1 (MQ=255)                                                                                          cGGCAACCAGGGGATGACCACCTTCATCGGCCGTATTGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCa                                         >  1:44806/1‑126 (MQ=255)                                                                                            gCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCaa                                >  2:214682/1‑133 (MQ=255)                                                                                                ccAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGcc                          >  8:143914/1‑135 (MQ=255)                                                                                                       aTGACCACCTTCATCGGCCGTCATGACTTCGTCAACCCGTTGCTCTCCGACCCGACCTGGGGTGTGTGCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTAc                   <  5:55907/135‑1 (MQ=255)                                                                                                       aTGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTAc                   >  1:175476/1‑135 (MQ=255)                                                                                                       aTGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGGTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTAc                   <  6:39655/135‑1 (MQ=255)                                                                                                             accTTCATCGGCCGTAATGCCTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCgctg             <  4:98595/135‑1 (MQ=255)                                                                                                             accTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCgctg             >  2:166146/1‑135 (MQ=255)                                                                                                                 tCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCgctgct           >  1:206650/1‑133 (MQ=255)                                                                                                                  cATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCgctgct           >  1:72128/1‑132 (MQ=255)                                                                                                                   aTCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCGCTGCTGCAg       <  7:69543/135‑1 (MQ=255)                                                                                                                   aTCGGCCGTAATGACTGCGTCAACCCGGGGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTACCACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCGCTGCTGCAg       <  1:196351/135‑1 (MQ=255)                                                                                                                    tCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCGCTGCTGCAg       <  1:166146/134‑1 (MQ=255)                                                                                                                    tCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCGCTGCTGCAg       <  3:183721/134‑1 (MQ=255)                                                                                                                       gCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCGCTGCTGCAGACGc   >  5:179871/1‑135 (MQ=255)                                                                                                                        ccGTAATGAGTTCGTCAACCCGGGGTTCTCCGCCCCGCCCGGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCGCTGCTGCAGACGCt  <  5:23874/135‑1 (MQ=255)                                                                                                                             |                                                                                                                                  GGCCTGCCGGCCCTGCTCAGCGCCCGTGACTACGACGATGTGTACGTCTACAACGGCGACTTTGCCTGGGACAACCAGTCCGGGTTCTTCGGCAACCAGGGGATGACCACCTTCATCGGCCGTAATGACTTCGTCAACCCGGTGTTCTCCGACCCGACCTGGGGTGTGTCCGACCAGGACATGTTCGACCGCGGCAACGAAGAGCTGGCCAAGCATGATGGCAAGAAGCCGATCTACGCGCTGCTGCAGACGCT  >  KT2440/2055292‑2055545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑