Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,230,919 A→T 9.8% V241D (GTC→GAC)  emrB_1 ← Colistin resistance protein EmrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,230,9190AT 9.8% 112.3 / 4.7 41V241D (GTC→GAC) emrB_1Colistin resistance protein EmrB
Reads supporting (aligned to +/- strand):  ref base A (18/19);  new base T (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

TGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTCCCAGTGCGCCTCCTT  >  KT2440/2230794‑2231052
                                                                                                                             |                                                                                                                                     
tGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGt                                                                                                                              >  4:163375/1‑135 (MQ=255)
           gACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTccagc                                                                                                                   >  6:10642/1‑135 (MQ=255)
                     aGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGTAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTg                                                                                                          >  1:62382/1‑134 (MQ=255)
                          cGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATg                                                                                                     <  2:47713/134‑1 (MQ=255)
                                 cGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCt                                                                                             <  5:175721/135‑1 (MQ=255)
                                   aGGATACGCAGTTTTATCAGAGGGTGATGCTTGTAAAACTGGAGGATGAGGTAGGTGATCATGCTGACCTGTGATACGCTGCCCAGGACGACTATCTGGTTCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCg                                                                                           <  4:13643/135‑1 (MQ=255)
                                     gATACGCATGTTGATCAGCGGGTTTGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCgag                                                                                         <  2:131237/135‑1 (MQ=255)
                                     gATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCgag                                                                                         <  3:118191/135‑1 (MQ=255)
                                               ttGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTgc                                                                               <  3:13180/135‑1 (MQ=255)
                                                 gATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTgcag                                                                             >  2:34668/1‑135 (MQ=255)
                                                 gATCAGAGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTTCAACGCTGCCCAGGCCGACGATCAGGTGCTACTCCAGCCAGTCCTTGCGATGGACTTCCTCGAGAAACACCTgcag                                                                             >  2:30110/1‑135 (MQ=255)
                                                    cAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTgcaggca                                                                          <  4:20901/135‑1 (MQ=255)
                                                    cAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTgcaggca                                                                          >  4:226444/1‑135 (MQ=255)
                                                    cAGCGGGGGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTgcaggca                                                                          <  2:177673/135‑1 (MQ=255)
                                                                cTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAg                                                              >  4:20240/1‑135 (MQ=255)
                                                                             aGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACg                                                 <  2:164878/135‑1 (MQ=255)
                                                                             aGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACg                                                 >  4:143321/1‑135 (MQ=255)
                                                                               gATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGTCGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGAt                                               >  4:209241/1‑135 (MQ=255)
                                                                               gATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGTCGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGAt                                               >  4:205592/1‑135 (MQ=255)
                                                                               gATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATg                                              >  8:109477/1‑136 (MQ=255)
                                                                                     gAAGGGGATCAGGCTGACCATTGGAACGATGCCCAGGCCGACGATCAGGTGCTACTCCTGCCAGTCCTTTCGATTGCCTTCCTCGAGAAACACCTCCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGc                                         <  6:167955/135‑1 (MQ=255)
                                                                                       aGGTGATCAGGCTGACCAGTGTAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCAt                                       <  2:62382/135‑1 (MQ=255)
                                                                                          tGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGTCGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAAt                                    <  3:205592/135‑1 (MQ=255)
                                                                                          tGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGTCGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAAt                                    <  3:209241/135‑1 (MQ=255)
                                                                                          tGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAAt                                    <  3:163375/135‑1 (MQ=255)
                                                                                          tGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAAt                                    <  4:169563/135‑1 (MQ=255)
                                                                                           gATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGa                                                                                                       <  4:241220/67‑1 (MQ=255)
                                                                                           gATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGa                                                                                                       >  3:241220/1‑67 (MQ=255)
                                                                                               aGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCACCCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTa                               >  3:9857/1‑135 (MQ=255)
                                                                                                    gACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTc                         <  7:10080/136‑1 (MQ=255)
                                                                                                             aaCGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTc                 >  5:53871/1‑135 (MQ=255)
                                                                                                             aaCGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTc                 >  6:244842/1‑135 (MQ=255)
                                                                                                             aaCGCTGCCCAGGCCGACGAGCCGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTc                 <  2:256770/135‑1 (MQ=255)
                                                                                                              aCGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTccc               >  8:109268/1‑136 (MQ=255)
                                                                                                                  tGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTCCCAGt            >  1:260769/1‑135 (MQ=255)
                                                                                                                    cccAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTCCCAGTg           <  7:109268/134‑1 (MQ=255)
                                                                                                                       aGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTgcaggca                                                                          >  1:143457/1‑68 (MQ=255)
                                                                                                                       aGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTgcaggca                                                                          <  2:143457/68‑1 (MQ=255)
                                                                                                                       aGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTCCCAGTGCGcct      >  7:60104/1‑136 (MQ=255)
                                                                                                                       aGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTAAGCAGCTCCCAGTGCGcc       >  1:183669/1‑135 (MQ=255)
                                                                                                                         gCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTCCCAGTGCGcctc     <  3:143321/135‑1 (MQ=255)
                                                                                                                            gacgaTCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTCCCAGTGCGCCTCctt  >  3:55081/1‑135 (MQ=255)
                                                                                                                             |                                                                                                                                     
TGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAGGTGATCAGGCTGACCAGTGCAACGCTGCCCAGGCCGACGATCAGGTGCGACTCCAGCCAGTCCTTGCGATGGCCTTCCTCGAGAAACACCTGCAGGCAGCCCAACCCAAGGCCGAGCGTGACGATGCCGGCATAATCGGTACTTTTCAGCAGCTCCCAGTGCGCCTCCTT  >  KT2440/2230794‑2231052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑