Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,417,619	A→C	24.7%	D44A (GAC→GCC)	yegS →	Lipid kinase YegS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,417,619	0	A	C	24.7%	56.1 / 4.3	47	D44A (GAC→GCC)	yegS	Lipid kinase YegS
Reads supporting (aligned to +/- strand):  ref base A (11/23);  new base C (5/8);  total (16/31)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCAAGGGCGCAAGGCAATGCTGGTGCTGCACGGCAAGCAGGCGATGAACGAAGACGTGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAGGCCATGGGCCTGGCCGCTACCCAGGCCAGCCTGGCC  >  KT2440/2417488‑2417746                                                                                                                                    |                                                                                                                                caTGCACGGGCGCAAGGCAAGGCGGGGGCTGCACGGCAAGCAGGCGATGAACGAACCCGTGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCGGGGTGCTGGATGTCCGGGTTCCCTGGGAGGCGGGCGACGcc                                                                                                                             <  8:59286/136‑1 (MQ=255)  aTGCACGGGCGCAAGGAAAGGCTGGGGCTGCACGGCAAGCTGGCGATGAACGAAGACGGGCGCAGCGCGGTGGGCGACCTGCGCTATAGGGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGcc                                                                                                                             <  2:160451/135‑1 (MQ=255)  aTGCAAGGGCGCAAGGCAATGCTGGTGCTGCACGGCAAGCAGGCGATGAACGAAGACGTGCGCAGCGCCGTGGGCGACCTGCGCGCTAGCGGCGGGGGGCTGGATGTACGGGTTACCTGGGAGGCGGGCgccgcc                                                                                                                             >  5:243855/1‑135 (MQ=255)     cAAGGGCGCAAGGCAATGCTGGTGCTGCACGGCAAGCAGGCGATGAACGAAGACGTGCGCCGCGCCGTGGGCGACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGGTACCTGGGAGGCGGGCGCCGCCCAAc                                                                                                                         >  8:240614/1‑136 (MQ=255)       aGGGCGCAAGGCAATGCTGGTGCTGCACGGCAAGCAGGCGATGAACGAAGACGTGCGCAGCGCCGTGGGCGACCGGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACg                                                                                                                        >  3:235706/1‑135 (MQ=255)        ggccgcAAGGAAAGGCGGGGGCTGCCCGGAAAGCAGGCGAGGAACGACGACGGGCGCCGCGCGGTGGGCGCCCGGGGCGATAGGGGCTGGGGGCGGGATGTCCGGGTACCCTGGGAGGCGGGCGACGCCCAACgg                                                                                                                       <  4:42285/132‑1 (MQ=255)                ggaaaTGCTGTTGCTGCACGGAAAGCAGGCTATGAACTAAGACGTGCGCAGCGCCGTGGGCGACCTGCGCTAAAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGc                                                                                                               <  4:195440/132‑1 (MQ=255)                    aTGCTGGTGCGGCACGGCAAGCAGGCGATGAACGACGACGTGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGCCGCCCAACGGCTGGTCGCCGAgg                                                                                                          <  8:170549/136‑1 (MQ=255)                             ctgcACGGCAAGCAGGCGATGAACGAAGACGTGCGCCGCGCCGTGGGCGACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGCCGCCCAACGGCTGGGCGCCGCGGCACTGGcc                                                                                                  >  2:220536/1‑134 (MQ=255)                             ctgcACGGCAAGCAGGCGATGAACGAAGACGTGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCa                                                                                                  >  4:41636/1‑135 (MQ=255)                               gcACGGCAAGCAGGGTATCAACGAAGACGTGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCTGGGGGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCg                                                                                               <  8:153565/136‑1 (MQ=255)                               gcACGGCAAGCAGGCGATGAACGAAGACGTGCGCAGCGCCGTGGGCGCCCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGCCGCCCAACGGCTGGTCGCCGAGGCACTGGCAGc                                                                                                >  4:272405/1‑135 (MQ=255)                                  gggCAAGTCGGCGATCAACTCAGCCGGGGGCAGCGCGGGGGGGGACCTGCGCGATCGGGGGGGGGTGCTGGATGTACGGGTAACCGGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCggg                                                                                             <  3:206482/134‑1 (MQ=255)                                  cGGCAAGCTGGCGATGAACGAAGCCGGGCGCAGCGCGGTGGGCGACCTGCGCGATCGCGGCTGGGTGCTGGATGTACGGGTTCCCTGGGCGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCggg                                                                                             <  4:67291/135‑1 (MQ=255)                                  cGGCAAGCAGGCGATGAACGAAGCCGTGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCGGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCggg                                                                                             <  1:50130/135‑1 (MQ=255)                                  cGGCAAGCAGGCGATGAACGAAGACGGGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCGGGGGGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCggg                                                                                             <  6:114430/135‑1 (MQ=255)                                  cGGCAAGCAGGCGAGGAACGAAGACGGGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCGGGGTGCTGGAGGTACGGGTTACCGGGGCGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCggg                                                                                             <  3:272405/135‑1 (MQ=255)                                   ggCAAGCAGGCGATGAACGAAGACGTGCGCAGCGCCGTGGGCGCCCTGCGCGCTAGCGGCGGGGTGGTGGCTGGACGGGTTACCGGGGCGGCGGGCGCCGCCCCACCGCGGGTCGCCGGCGCACTGGCCGCggg                                                                                             >  2:126039/1‑134 (MQ=255)                                   ggCAAGCAGGCGATGAACGAAGACGTGCGCAGCGCCGTGGGCGACCAGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTTCCTGGGAGGCGGGCGCCGCCCAACGGCTGGTCTCCCTCGCACAGGCAGCggg                                                                                             >  2:67173/1‑134 (MQ=255)                                                tGAACGAAGACGTGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATAtcg                                                                               >  1:22448/1‑135 (MQ=255)                                                   accacgACGGGCGCTGCGCGGGGGGGGACCTGCGCTATAGGGGCGGGGTGCTGGATGTACGGGTAACCGGGGAGGCGGGCGCCGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATAtcgt                                                                              <  4:235706/128‑1 (MQ=255)                                                               gccgcgcCGTGGGCGACCGGCGCGATAGCGGCGGGGTGCTGGATGTACGGGTTACCTGGCAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGa                                                               <  7:264990/133‑1 (MQ=255)                                                               gcAGCGCCGGGGGCGACCGGCGCGATAGCGGGTGGGGGCGGGATGTACGGGTTCCCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCGACAGCCATATCGTCGCCGGTGGCGGGGa                                                               <  8:130300/136‑1 (MQ=255)                                                                 aGCGCCGGGGGCGACCTGCGCGATAGCGGCTGGGGGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGAc                                                              <  2:224205/135‑1 (MQ=255)                                                                    gcCGGGGGCCACCGGCGCTATCGCGGCTGGGGGCTGGATTTCCGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGc                                                           <  6:238749/135‑1 (MQ=255)                                                                    gaggggggCGAGGTGCCCGATTGGGGCTGGGGGCTGTTTGTCCGGGTTCCCTGGGCGGCGGGCGACCCCAAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGc                                                           <  1:67173/130‑1 (MQ=255)                                                                         gggCGACCGGCGCGATAGCGGGGGGGGGCGGGAGGTCCGGGTCCCCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGtt                                                      <  5:50020/135‑1 (MQ=255)                                                                           gCGACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCg                                                   >  7:33706/1‑136 (MQ=255)                                                                             gACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCg                                                   >  1:234911/1‑134 (MQ=255)                                                                                cTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCg                                                                                               <  8:133929/87‑1 (MQ=255)                                                                                cTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCg                                                                                               >  7:133929/1‑87 (MQ=255)                                                                                  gcgcGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGc                                                                                      >  4:118787/1‑94 (MQ=255)                                                                                  gcgcGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGc                                                                                      <  3:118787/94‑1 (MQ=255)                                                                                    ggtttcgcggcgGGGGGCGGGATGTCGGGGTTCCGTGGGAGGCGGCCGCCGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTgg                                           <  1:118191/129‑1 (MQ=255)                                                                                    gcgATAGCGGGTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTgg                                           <  2:22448/135‑1 (MQ=255)                                                                                    gcgATAGCGGCGGGGGGCTGGATGTACGGGTTCCCGGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTgg                                           <  1:190997/135‑1 (MQ=255)                                                                                          gCGGCTGGGGGCTGTATGTCCGGGGCCCGTGGGAGGCGGGCGCCGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAgg                                     <  5:260590/135‑1 (MQ=255)                                                                                           cggggggggGCTGGATGTCCGGGTTACCGGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAgg                                     <  6:243855/129‑1 (MQ=255)                                                                                               tGGGTGCGGGAGGTCCGGGTTACCGGGGAGGCGGGCGCCGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGGCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAGGCCATg                                <  1:209995/135‑1 (MQ=255)                                                                                                   tGCTGGATGTACGGTTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGTCACGTTGCGTGATGTGGCCGAGGCCATGGGCCt                           >  7:125765/1‑136 (MQ=255)                                                                                                     cTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAGGCCATGGGCCTgg                         >  7:253051/1‑136 (MQ=255)                                                                                                          ggtccGGGTTCCCTGGGCGGCGGGCGACGCCCAACGGCTGGGCGCCGAGGCACGGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAGGCCATGGGCCTGGCCGc                     <  1:168024/131‑1 (MQ=255)                                                                                                               gggTTACGGGGCTGGCGGGGGCGGCGCAAGGGCTGGTCGCGGTGGCACTGGCAGCGGGCTACTGCCTTATCGTCGCGGGTGGCGGGGACGGAACGTTGCGTTATGTGGCCGAGGCCATGGGCCTGGCCGCTAccc                <  6:231096/135‑1 (MQ=255)                                                                                                                     cgggggCGGCGGGCGCCGCCCAAGGGGTGGGCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTGGCGTGATGGGGCCGAGGCCAGGGGCCTGGCCGCTACCCAGGCCa          <  4:54813/132‑1 (MQ=255)                                                                                                                     ccTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAGGCCATGGGCCTGGCCGCTACCCAGGCCa          >  3:24799/1‑135 (MQ=255)                                                                                                                       tGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAGGCCATGGGCCTGGCCGCTACCCAGGCCAGc        >  2:759/1‑135 (MQ=255)                                                                                                                             gcgggcgACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGGGGCCGAGGCCATGGGCCTGGCCGCTACCCAGGCCAGCCTGGcc  <  6:77229/135‑1 (MQ=255)                                                                                                                             gcgggccCCCCCCAACGGCGGGGCGCCGCGGCACTGGCCGCGGGCTACAGCCATAGCGGCGCCGGGGGCGGGGACGGCAGGGGGCGTGATGTGGCCGAGGCCAGGGGCCGGGCCGCTACCCAGGCCAGCCTGGcc  <  5:213653/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                CATGCAAGGGCGCAAGGCAATGCTGGTGCTGCACGGCAAGCAGGCGATGAACGAAGACGTGCGCAGCGCCGTGGGCGACCTGCGCGATAGCGGCTGGGTGCTGGATGTACGGGTTACCTGGGAGGCGGGCGACGCCCAACGGCTGGTCGCCGAGGCACTGGCAGCGGGCTACAGCCATATCGTCGCCGGTGGCGGGGACGGCACGTTGCGTGATGTGGCCGAGGCCATGGGCCTGGCCGCTACCCAGGCCAGCCTGGCC  >  KT2440/2417488‑2417746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑