Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,471,589	G→A	20.0%	L45L (CTG→CTA)	CJ019_02330 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,471,589	0	G	A	20.0%	32.9 / 4.7	20	L45L (CTG→CTA)	CJ019_02330	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (14/2);  new base A (2/2);  total (16/4)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

GCGGTGGCGGTGGTCATGGCGGTGGAAACGGTGCCGGACATGGCGGTGGCATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTGGCCAAGTCCACG  >  KT2440/2471491‑2471720                                                                                                   |                                                                                                                                    gcggtggcggtgGTCATGGCGGTGGAAACGGTGCCGGACATGGCGGTGGCATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATc                                                                                                 >  3:62165/1‑135 (MQ=255)  cggtggcggtggTCATGGCGGTGGAAACGGTGCCGGACATGGCGGTGGCATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACCCGCGATCATGGGGTAAGCGGCAATCAt                                                                                               >  8:9635/1‑136 (MQ=255)                          aaaCGGTGCCGGACATGGCGGTGGCATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCg                                                                         >  1:272796/1‑134 (MQ=255)                          aaaCGGTGCCGGACATGGCGGTGGCATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGAAACAGCg                                                                         >  5:186180/1‑134 (MQ=255)                                    ggACATGGCGGTGGCATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCAt                                                              >  2:152169/1‑135 (MQ=255)                                             ggTGGCATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGcaccacc                                                     >  4:199024/1‑135 (MQ=255)                                                  cATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTc                                                >  2:131650/1‑135 (MQ=255)                                                  cATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTc                                                >  6:129070/1‑135 (MQ=255)                                                         gggCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTc                                         >  3:37836/1‑135 (MQ=255)                                                               aGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCAc                                   >  1:79177/1‑135 (MQ=255)                                                                    aaGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACACCGGTCATGGCACAACCTCCTCGGGTGTCGCTCACTCCaa                              >  3:143300/1‑135 (MQ=255)                                                                       cGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAgg                            >  3:240613/1‑134 (MQ=255)                                                                                     gAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTg              <  4:62165/134‑1 (MQ=255)                                                                                     gAAGTGATCACGCTAGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTgg             >  3:128209/1‑135 (MQ=255)                                                                                     gAAGTGATCACGCTAGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTgg             >  3:123597/1‑135 (MQ=255)                                                                                            tCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTGGCGAAGTc      >  3:153013/1‑135 (MQ=255)                                                                                              aCGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTGGCCAAGTCCAc   >  8:10142/1‑136 (MQ=255)                                                                                                gCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTGGCCAAGTCCACg  <  2:79177/135‑1 (MQ=255)                                                                                                gCTAGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTGGCCAAGTCCACg  <  4:123597/135‑1 (MQ=255)                                                                                                gCTAGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTGGCCAAGTCCACg  <  4:128209/135‑1 (MQ=255)                                                                                                   |                                                                                                                                    GCGGTGGCGGTGGTCATGGCGGTGGAAACGGTGCCGGACATGGCGGTGGCATGGGAGGGCATAGTGGAAGCGGGAAGGGCCTTGGAAGTGATCACGCTGGAAAGGCGACACGCGATCATGGGGTAAGCGGCAATCATTACGGAAGCTCGCGCAACAGCGACAACGGTCATGGCACCACCACCTCGGGTGTCGCTCACTCCAAGGACACTCGCGGTTTGGCCAAGTCCACG  >  KT2440/2471491‑2471720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑