Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,595,295 T→C 9.7% M1T (ATG→ACG) † CJ019_02454 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,595,2950TC 9.7% 113.5 / 4.5 41M1T (ATG→ACG) †CJ019_02454hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/17);  new base C (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

GAAGAGGGCGCAGTAACAGAGCCCCTTATCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACATCACGACCCCTGCTGAAACCGTAGAG  >  KT2440/2595163‑2595428
                                                                                                                                    |                                                                                                                                     
gAAGAGGGCGCAGTAACAGAGCCCCTTATCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTGTAATg                                                                                                                                      >  2:103617/1‑134 (MQ=255)
  agagGGCGCAGTAACAGAGCCCCTTATCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGCAGCCCTTTAATgg                                                                                                                                     >  2:78896/1‑133 (MQ=255)
  agagGGCGCAGTAACAGAGCCCCTTATCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGa                                                                                                                                    >  7:152394/1‑134 (MQ=255)
   gagGGCGCAGTAACAGAGCCCCTTATCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGaaa                                                                                                                                  <  3:259705/135‑1 (MQ=255)
         gcAGTAACAGAGCCCCTTATCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATc                                                                                                                             >  2:80878/1‑134 (MQ=255)
                       ccTTATCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCg                                                                                                              <  1:80878/135‑1 (MQ=255)
                           aTCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGAACTTTAATGGAAAGCAGCGAAATCGTATCACCGAAccc                                                                                                         >  7:231881/1‑136 (MQ=255)
                               aaTACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGt                                                                                                       <  1:201240/134‑1 (MQ=255)
                                  aCTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGc                                                                                                   >  5:102214/1‑135 (MQ=255)
                                    ttCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGccc                                                                                                 >  4:269514/1‑135 (MQ=255)
                                         tGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCACAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCACCGAg                                                                                            >  3:167907/1‑135 (MQ=255)
                                               cgcAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCat                                                                                      <  6:91078/135‑1 (MQ=255)
                                                    agCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAg                                                                                >  7:24864/1‑136 (MQ=255)
                                                    agCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAg                                                                                >  7:29237/1‑136 (MQ=255)
                                                            caattcaaCATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGa                                                                         >  4:110259/1‑135 (MQ=255)
                                                                 caaCATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCTGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTgcc                                                                    >  1:176284/1‑135 (MQ=255)
                                                                 caaCATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTgccg                                                                   >  8:112370/1‑136 (MQ=255)
                                                                 caaCATCAACCCCCAGGCCAAGCGATACCGAGTGTTAGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCCTTCTGAGGCAATGATTgcc                                                                    >  3:198288/1‑135 (MQ=255)
                                                                  aaCATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTgccg                                                                   <  8:92183/135‑1 (MQ=255)
                                                                       cAACCCCCAGGCCCAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATc                                                              <  5:189329/135‑1 (MQ=255)
                                                                       cAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCAAGATGAGGCAATGATTGCCGCCATc                                                              >  1:174973/1‑135 (MQ=255)
                                                                        aaCCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGGTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATc                                                              <  8:256570/134‑1 (MQ=255)
                                                                           ccccAGGCCAAGCGATACCGAGTGGTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACaa                                                         <  8:152394/136‑1 (MQ=255)
                                                                            cccAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACaa                                                         <  5:130884/135‑1 (MQ=255)
                                                                                       cGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTACTGGAAAGCAGCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTg                                              <  5:39506/135‑1 (MQ=255)
                                                                                                      gCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGCGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGaca                               >  6:168796/1‑135 (MQ=255)
                                                                                                      gCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGac                                <  1:169517/134‑1 (MQ=255)
                                                                                                       cTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGaca                               >  6:211050/1‑134 (MQ=255)
                                                                                                           tGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCAGCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACAt                           <  2:202720/134‑1 (MQ=255)
                                                                                                              ccGCGTTCTAGAATAACTTTAATGGAAAGCATCGAACTCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACATCACg                       <  4:167907/135‑1 (MQ=255)
                                                                                                                  gTTCTCGAAGACCTTTAATGGAAAGAATCGAAATCGTATCCCCGACCCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACATCACGAcccc                  <  7:253525/136‑1 (MQ=255)
                                                                                                                    tctcGAAGACCTTTACCGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATc                                                              <  5:6347/90‑1 (MQ=255)
                                                                                                                    tctcGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACATCACGCCCCct                 >  2:189702/1‑135 (MQ=255)
                                                                                                                    tctcGAAGACCTTTAATGGAAAGCATCGAAATAGAATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCAACGACAAGGCCAATGCTGGCCCGGTAGGCGACAACCTCACGACCCct                 >  4:17535/1‑135 (MQ=255)
                                                                                                                    tctcGAAGACCTTTAACGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATc                                                              >  6:6347/1‑90 (MQ=255)
                                                                                                                    tctcGAAGACCTTTAACGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATc                                                              >  6:2668/1‑90 (MQ=255)
                                                                                                                    tctcGAAGACCTTTAACGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATc                                                              <  5:2668/90‑1 (MQ=255)
                                                                                                                         aaGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACATCACGACCCctgc               <  1:4498/132‑1 (MQ=255)
                                                                                                                             ccTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGCGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACATCACGACCCCTGCTGAAAcc        <  1:78896/135‑1 (MQ=255)
                                                                                                                                  aaTGGAAAGCATCGAAAACGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATTATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACATCACGACCCCTGCTGAAACCGTa     <  2:114133/133‑1 (MQ=255)
                                                                                                                                   aTGGAAAGCATCGAAATAGTATCCCCGAACCCGTCTGACCCCGAGGGGCATGATGAGGCAATTATTGCCGCCATCGACAAGGCAAATGCTTGCCCGGTAGGCGACAAAATCACGACCCCTGCTGAACCCGTagag  >  1:125447/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GAAGAGGGCGCAGTAACAGAGCCCCTTATCGAATACTTCGGTGAGGGCGCAGAGCAAGTTCAATTCAACATCAACCCCCAGGCCAAGCGATACCGAGTGTTCGCTAATGGCCGCGTTCTCGAAGACCTTTAATGGAAAGCATCGAAATCGTATCCCCGAACCCGTCTGCCCCCGAGGGGCATGATGAGGCAATGATTGCCGCCATCGACAAGGCCAATGCTGGCCCGGTAGGCGACAACATCACGACCCCTGCTGAAACCGTAGAG  >  KT2440/2595163‑2595428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑