Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,759,723 A→T 7.7% L665* (TTG→TAG)  cntO_2 ← Metal‑pseudopaline receptor CntO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,759,7230AT 7.7% 156.8 / 3.9 52L665* (TTG→TAG) cntO_2Metal‑pseudopaline receptor CntO
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base T (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

AGAAGCTGTAATCCACCGTCGCGAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCTCATCCACATAGCGCGCCCCAATGCCGGCCTCCACCTGCTGGCCGAAACCT  >  KT2440/2759592‑2759851
                                                                                                                                   |                                                                                                                                
aGAAGCTGTAATCCACCGTCGCGAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAaca                                                                                                                               >  4:141321/1‑135 (MQ=255)
   accTGTAATCCACCGTCGCGAACTACCACCCAGCTATACACATCAACCACTGCTGCCCGCAGCACGCCGACTGTACATACTAGCGATCGAACAGGGTGTTCAACGGCAACCCCACCATCACATCAGGCAACACCTg                                                                                                                           >  8:123293/3‑136 (MQ=255)
          aTCCACCGTCGCGAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCg                                                                                                                    >  8:42847/1‑136 (MQ=255)
           tCCACCGTCGCGAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCg                                                                                                                    <  2:181031/135‑1 (MQ=255)
           tCCACCCTCGCGAAGTACGACAGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTGACATCCTGCAACACCTGCCAGGCg                                                                                                                    <  3:180955/135‑1 (MQ=255)
                  tCGCGAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTGCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCTATGTTgg                                                                                                             <  4:71607/135‑1 (MQ=255)
                     cgAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCAt                                                                                                          >  5:246780/1‑135 (MQ=255)
                     cgAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATc                                                                                                         >  8:96471/1‑136 (MQ=255)
                      gAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATc                                                                                                         <  5:72647/135‑1 (MQ=255)
                      gAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATc                                                                                                         <  6:271357/135‑1 (MQ=255)
                          tACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGAcc                                                                                                     <  6:246853/135‑1 (MQ=255)
                          tACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGAcc                                                                                                     <  6:246780/135‑1 (MQ=255)
                          tACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGAcc                                                                                                     <  5:162020/135‑1 (MQ=255)
                                     tATCCCCATCACCCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGCCCACGGTGTACCCg                                                                                         >  8:236908/1‑136 (MQ=255)
                                     tATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTAccc                                                                                          >  2:22859/1‑135 (MQ=255)
                                     tATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCg                                                                                         <  8:176583/136‑1 (MQ=255)
                                               aaCCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGctc                                                                                >  6:14224/1‑135 (MQ=255)
                                                      gctgcCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTAt                                                                         >  3:32209/1‑135 (MQ=255)
                                                       ctgcCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTAtt                                                                        >  3:170064/1‑135 (MQ=255)
                                                         gcCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTgg                                                                      >  3:247419/1‑135 (MQ=255)
                                                             gCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCgg                                                                   <  7:273718/134‑1 (MQ=255)
                                                             gCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCgg                                                                   >  8:273718/1‑134 (MQ=255)
                                                                  aCGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCTACACCTGCCAGGCGATGTTg                                                                                                              >  7:271728/1‑86 (MQ=255)
                                                                  aCGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCTACACCTGCCAGGCGATGTTg                                                                                                              >  7:268387/1‑86 (MQ=255)
                                                                  aCGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCTACACCTGCCAGGCGATGTTg                                                                                                              <  8:268387/86‑1 (MQ=255)
                                                                  aCGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCTACACCTGCCAGGCGATGTTg                                                                                                              <  8:271728/86‑1 (MQ=255)
                                                                  aCGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCg                                                              >  2:170490/1‑134 (MQ=255)
                                                                  aCGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCg                                                              <  2:75433/134‑1 (MQ=255)
                                                                  aCGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCGCTGGGTATTGGCGGGATCgg                                                             >  2:113228/1‑135 (MQ=255)
                                                                    gCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCa                                                           <  4:188187/135‑1 (MQ=255)
                                                                      cGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATa                                                         <  4:105497/135‑1 (MQ=255)
                                                                       gACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAAc                                                       <  7:141966/136‑1 (MQ=255)
                                                                        aCTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAAc                                                       <  4:247419/135‑1 (MQ=255)
                                                                        aCTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAAc                                                       <  1:164007/135‑1 (MQ=255)
                                                                          tGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGc                                                     <  3:112457/135‑1 (MQ=255)
                                                                           gTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGa                                                                                                       <  6:669/84‑1 (MQ=255)
                                                                           gTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGa                                                                                                       >  5:669/1‑84 (MQ=255)
                                                                           gTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCt                                                    >  7:152237/1‑135 (MQ=255)
                                                                                         tCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGt                                                                           <  6:39348/98‑1 (MQ=255)
                                                                                         tCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGt                                                                           >  5:39348/1‑98 (MQ=255)
                                                                                         tCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCgg                                                                   <  6:168562/106‑1 (MQ=255)
                                                                                         tCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCgg                                                                   >  5:168562/1‑106 (MQ=255)
                                                                                         tCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCTCATCCACATAgcgcg                                     >  7:117232/1‑136 (MQ=255)
                                                                                         tCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCTCATCCACATAgcgcg                                     <  7:123293/136‑1 (MQ=255)
                                                                                                          cTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATa                                                         >  1:202359/1‑99 (MQ=255)
                                                                                                          cTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATa                                                         <  2:202359/99‑1 (MQ=255)
                                                                                                              aaCCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCTCATCCACATAGCGCGCCCCAATGCCGGCCTCCAcc                 <  4:267723/135‑1 (MQ=255)
                                                                                                                ccccAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCTCATCCACATAGCGCGCCCCAATGCCGGCCTCCACctgc              >  8:267045/1‑136 (MQ=255)
                                                                                                                     aCTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCTCATCCACATAGCGCGCCCCAATGCCGGCCTCCACCTGCTGGc          <  5:14224/135‑1 (MQ=255)
                                                                                                                        tCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCt                                                    >  6:155178/1‑90 (MQ=255)
                                                                                                                        tCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCt                                                    <  5:155178/90‑1 (MQ=255)
                                                                                                                             tCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCTCATCCACATAGCGCGCCCCAATGCCGGCCTCCACCTGCTGGCCGAAACCt  >  6:255620/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                
AGAAGCTGTAATCCACCGTCGCGAAGTACGACCGCGGTATCCCCATCAACCACTGCTGCCCGCTGAACGCCGACTGTACATACTCGCGATCGAACAGGTTGTTCAACTGCAACCCCAACTTCACATCCGGCAACACCTGCCAGGCGATGTTGGCATCGACCACGGTGTACCCGGGGGCGCTCTGGGTATTGGCGGTATCGGCATAACGCTCATCCACATAGCGCGCCCCAATGCCGGCCTCCACCTGCTGGCCGAAACCT  >  KT2440/2759592‑2759851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑