| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,061,793 | G→A | 100% | S592N (AGC→AAC) | qedA_2 → | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,061,793 | 0 | G | A | 100.0% | 50.7 / NA | 16 | S592N (AGC→AAC) | qedA_2 | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/3); total (13/3) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGG > KT2440/3061665‑3061909 | ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCCGCGAAGTGGCCAAACGGGTCAAGGACTTAAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCaa > 1:44738/1‑135 (MQ=255) gTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc < 8:166533/136‑1 (MQ=255) gTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCt < 3:5300/135‑1 (MQ=255) cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATc > 5:40008/1‑135 (MQ=255) cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCg > 8:119678/1‑136 (MQ=255) aaGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTTCTGCCGCGGCTCGGTg > 6:122901/1‑134 (MQ=255) aaaCGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGcc > 7:39992/1‑136 (MQ=255) aaaCGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGc > 2:160666/1‑135 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCGTGCTGCCGCGGGTCGGTGGCCAGCGGCCCACCGACCAACTGAcc > 3:142107/1‑132 (MQ=255) gcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGACCCCCGCt > 3:243044/1‑135 (MQ=255) cTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTTATCGGTGGCAACCGGCCCTACTACCAAATCACGAGAGCCCCGCTGCCAACGATGCAtt > 7:154508/1‑136 (MQ=255) cTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCAt > 4:254887/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTTCTTCCGCTGATCGGTGGCAAGCGGCCCGACTACCAAAAGACCACAGCCCCCCAGCCAACCATGCATTAGcc > 5:202725/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcc > 3:181966/1‑135 (MQ=255) cccAAGCAGTTGCACCAACCGGCAAACGTCAACCCAAAACGCCTGCCTACTGCCGCTGGCTGCCGCTGATCGGGGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGc < 6:138019/134‑1 (MQ=255) aGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACGACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCgggg > 6:103844/1‑135 (MQ=255) | GGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGG > KT2440/3061665‑3061909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |